LOCUS       sequence-22409-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Wassmann K, Liberal V, Benezra R
  TITLE     Mad2 phosphorylation regulates its association with Mad1 and the 
            APC/C.
  JOURNAL   EMBO J. 2003 Feb 17. 22(4):797-806.
  PUBMED    12574116
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2003 Feb 17. 22(4):797-806."
COMMENT     Sequence Label: VL3 (Flag-Mad2)
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgga ccgtcagaat tgatctacca tggactacaa agacgatgac
       61 gacaagctta ccatggcgct gcagctctcc cgggagcagg gaatcaccct gcgcgggagc
      121 gccgaaatcg tggccgagtt cttctcattc ggcatcaaca gcattttata tcagcgtggc
      181 atatatccat ctgaaacctt tactcgagtg cagaaatacg gactcacctt gcttgtaact
      241 actgatcttg agctcataaa atacctaaat aatgtggtgg aacaactgaa agattggtta
      301 tacaagtgtt cagttcagaa actggttgta gttatctcaa atattgaaag tggtgaggtc
      361 ctggaaagat ggcagtttga tattgagtgt gacaagactg caaaagatga cagtgcaccc
      421 agagaaaagt ctcagaaagc tatccaggat gaaatccgtt cagtgatcag acagatcaca
      481 gctacggtga catttctgcc actgttggaa gtttcttgtt catttgatct gctgatttat
      541 acagacaaag atttggttgt acctgaaaaa tgggaagagt cgggaccaca gtttattacc
      601 aattctgagg aagtccgcct tcgttcattt actactacaa tccacaaagt aaatagcatg
      661 gtggcctaca aaattcctgt caatgactga ggtaccagga ttcccgggtg gcatccctgt
      721 g
//
