LOCUS       sequence-224103-       15028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU64.1-2.2
FEATURES             Location/Qualifiers
     source          1..15028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             272..295
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9220..9240)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9220..9238)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9577..10165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9657..9676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10336..11127,11128..11196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10959..10978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11197..11301)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11328..12670
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12291..12338)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12711..12729
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13024..13431
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13432..14526
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14773..14789)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14773..14789)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ataagagaat ttcagattga gagaatgaaa aaaaaaaaaa aaaaaaggca gaggagagca
       61 tagaaatggg gttcactttt tggtaaagct atagcatgcc tatcacatat aaatagagtg
      121 ccagtagcga cttttttcac actcgaaata ctcttactac tgctctcttg ttgtttttat
      181 cacttcttgt ttcttcttgg taaatagaat atcaagctac aaaaagcata caatcaacta
      241 tcaactatta actatatcgt aatacacaat ggattataag gatgatgatg ataagactag
      301 tctgggtagc tatccagaga ccaacctcct ctccatccca gagcccatcg ccattgtagg
      361 aacctcatgt cggtttccag gtggtgcaga ctctccatct aaactatgga gtctcctcaa
      421 gaaccctcaa gacattgtgc aagagattcc tccgagtcgc ttcgacacga aagggttcta
      481 caacaaagac agccaacacc acggtagctt gaacgcacga cacgcatatc ttttgcaaga
      541 tgatccccga gcattcgacc gcgacttctt caacatcagc cccaaggaag ccgaggccat
      601 ggatccccaa caacgctgtc tcctcgagac agtctatgaa ggcgttgaat cggccggata
      661 ctcgataccc cagctgcaag gctccgcgac aggtgtcttt gtaggcgcca tgagcttcga
      721 ctaccaattt gttgctatgc gaggcttcga tagcttgcca cagtaccacg caaccggtac
      781 ttccatgtcg atcctggcga atcgcctctc ctacttcttt gactggagag ctgttcttgc
      841 gctgcggaac ggtgatgtta gtatggctgt tgctgccggc gctaatctta ttctcgggcc
      901 tgagccgttt atcgccctgt caaacctgac gaaggaattg aagttaaaca tgctgtcacc
      961 caacggccgt tcatacatgt gggattcatc cggcgacggc tacacacgag gcgaaggctt
     1021 cgctgccgtt attctcaaga ctcttagtca agccctagcc gacggtgacc acattgaatg
     1081 tatcatccgt gagactggcg taaactcgga tgggcggact ccgggcatca ccatgccgag
     1141 cgcaacagcg caggctcggc ttatccgaga gacataccaa aggtgtgggc tagacataaa
     1201 cagtccactt gatcgcccac agtactttga ggcccatggt accggcacac cggcaggaga
     1261 tcctatcgag gcccgagcta tacatgaagc tttcttttct catagtccgg aaccccatga
     1321 cgaccaggca ccactctacg tcggctccat caagactgtt cttggccaca ctgaaggcac
     1381 tgccggcctc gctggagtcc ttaaggcatc tctggcagtt cagaacggtt caattccagg
     1441 aaatctccat tttaaaaacc tgaatcccaa gatctggcct ttctacgaca aactgcgcgt
     1501 ccccaccaca ttaacgtcat ggcctagcct tccagacggc cagcctcacc gcgtcagcgt
     1561 caacagcttt ggctttggag gaaccaacgc gcacgccatt atcgaaagct gggatgagaa
     1621 gcccgagcgg tgtcctttga gaactgcaga acatggcgga cttgttgtgt tatctgccaa
     1681 ttccgcacag gcattggcag caaaggctac gtcgctggct gcataccttc gaaagcaccc
     1741 tgatacagat atcgaccggc tctcacgcac catgttccag cgcgctgatt tcctataccg
     1801 agccgccttt gcagccacgt cgactgccca acttgcagag aaacttgaag cacgaactga
     1861 gatgttgaaa aagacttccc gcattgcggc aattcctaac cacttgccgc cccgcatcct
     1921 tggtgtcttt accgggcaag gcgcgcagtg ggctactatg ggcagggaac tgtacgatgc
     1981 atcgcctgtg ttccgcaaat ccatggaccg gctgcagcga agccttgaca cgcttcctca
     2041 tggcgaacgc ccagactgga ctcttatcga cgagctttcg ctgtccaaag agacgtctcg
     2101 tattggtcaa gccgccatct cgcagccact gtgtactgct ttgcagatcg ctctcatcga
     2161 tgtcttgcac gtcatcggca tctcctttgc tgccgttgtt ggccactctt ctggagagat
     2221 tgcctgtgcc tatgcagcag ggtacctaga tgcacaggac gccatccgcg ttgcttatta
     2281 ccggggattc cactcacacc tctccaaggg ccccgacgag aaacgcggca aaatgatggc
     2341 tgtgggactg agtctcgagc aagcgtcagc cttctgtagt gagttcgggt ctgcgctcaa
     2401 agtggctgct agcaactctc ccaccagttg cacactggcc ggcgatgcgg acgccattga
     2461 tgaagccaaa gagcggctag agcaggaaaa gacttttgct cgtgtgctag ctgtagacac
     2521 cgcataccat tctcatcaca tgcttccctg cgccgaaccc tatctacagt cgctacgcaa
     2581 ctgtggagtc cgagctctca ggggtccaaa gcgatgtgct tggtactcca gtgtctgggg
     2641 ccccaatgga cgcagccgct ctttcgatag caaagatagc atggagttgc tcgaaggcca
     2701 gtactgggtc gataacctaa ccaacacggt ccagttctcg caagcagtat accgagctat
     2761 ttccgaagag cccttcatca tggacctcgc actggaagtt ggaccccacc ctgctctcaa
     2821 gggacccagc tctgaagtca tgaagtcgct gactggggtt tccgttccat atagcggtat
     2881 tttgaaacgc ggagagaatg ccgtcgaagc gtttgccgat gctctaggac tcatctggac
     2941 gtctttccca tctcagaacc ctatcgtcac gttcgatggg atgcatcgcg ccttgccaca
     3001 agctggacca aagaagccta gcatcctgaa gaacttgcca ggttacccat gggaccatga
     3061 tagcctcatc tggcgtgaat cgagggcttc tcgcgtgttc cgctctcaga accagccaag
     3121 acacgagctc ctcggtcacc ctgtaacgtt gggcgaacat agcaggcgag aggtacactg
     3181 gcgccagatc ttcaagctgg gcgagcttcc ttgggtgact ggtcataata tccaaggcga
     3241 agtacttttc ccagccacgg ggtacctcac aatggcgtac gaagccgcta tacgccttgt
     3301 agacaatgag acagcgctgc gcctcgtcga gttgcacgat gtggagattg ttcgcgctat
     3361 gggtttgcca gaagattccc ccggcctcga ggttctcttc actgtgtccg ttacaggcca
     3421 gtctgacgac tgcgtcacgg caaaagtggc ttgctacagc ggcaacgtca acgtagccaa
     3481 gctggatagt cctcttcatg gactcacagc tcatttctca gctggcgtta gactttggct
     3541 cggtgaaccc agcaatagtt ccctacctgg ccgcaacaga cccctgctcc ccatgaatac
     3601 cctcgatatg gagcagttct actcctacct gtcgaaggta ggatacaact acgcgctgcc
     3661 tttcagagca accagtgtgg accgccatct caaccacgcg gtcgtgacgc ttccatcgcc
     3721 tccagagacg tcttcgtcta ttcgtgatgt tacgcatccc gtagtcctcg acactgcgat
     3781 tcaaggagtc ttggccgcat tttctttccc tgaagacggc cgcctggaca ctgtgtatct
     3841 ccccacacgc atcgactgcg tccgcatcaa cacatcgtca acatataata gccccttgat
     3901 ggcagacagc gtcttgacat cgaccgaagc aaaggcactc aagggcgaca tcgaggtgtt
     3961 cagcgagagc gacgcagccg tccacgtgca aatgcgtggc gtccactaca ctgcgctgaa
     4021 ccaaaacaaa gaccgatggc tgtacgcaag cgagaactgg gtgcgcgatg caatccacgg
     4081 tattgagccg agccagatga ccaagctttc tcctgaagcc gaggcgtttg agaagctcct
     4141 gacacgcata gcgtatttct atctgcgcaa gctccgcgat cagatcaagc cgtcggagct
     4201 attgctcatg agcaagcacc gacgccacat gatgaagtgg gtcagagagc atctgtttcc
     4261 ccagatcgag gcaggccagc gtccagatgt cgaagtggaa tgggtaaacg acaccctgga
     4321 ggatgtcgag cggtggagcg ctagttacct tgcagctggc aacaacgaca tgcaactgct
     4381 tcatgccgtc gggaagaatc tgcctgctat tgcccgcgga acgaagcctg cgctacaggt
     4441 gttgctgacc gacgacatgc tggaccgtct gtacgtcgaa ggcactggaa tcgcggatgc
     4501 taacctcgac tttgccctgt tggtgcagca gattgcacac cgctacccgc gcatgaatgt
     4561 catcgaggtg ggtgccggaa caggcggtac cacgcgagcg gtgctgtcct ctttgggcga
     4621 tcagtatgcg tcgtacactt ataccgacat ctcagccgga tttttcgagc ctgcaaaggc
     4681 aaaattcagc cagcacggcg gaaagctcaa gttcaaaaca ctcaacattg aaaaggaccc
     4741 catgggccaa ggctttgatg aaggctcgtt tgacatggtt attgcttcaa attgtctcca
     4801 cgctactcgc agtctccagg aaacactgcg ccactgccgc cgcttgctcc ggcccggagg
     4861 ctacttagtc ctgctggaaa tcactcgcga ccatctccct attgaggagt gggacgcgtt
     4921 gctcaaggca aacaggttct ctggagtaac tgcaagctcg acgccttctt attgctctgt
     4981 gataatgtcg caggcagttg atgatacctt ccagctactc caggggccgc tctctgtcac
     5041 ttctcagcag cagccgttgg catccagagg accggtattg gttgttggtg gtaacaagtc
     5101 aggacttggt gccaggtctc aggaaatact tgcttctcac ggtgcatcat acctgcatga
     5161 gcttgagggc atcgaggtgc cgagtggagc agtcgttctc tgtctatctg acttggacaa
     5221 ccccatcttt agtgtcatga cagaggctgc gttccagggt atacaaacaa tcttccggaa
     5281 cgcaagcatt gttctgtggg tgacgtctgg agcggcttcg ggcaaaaatc caatggcgaa
     5341 cgtaacggtc ggattaggac gaacactgct tgccgaatgc agtgacttga gactccagtt
     5401 ccttgacgcg gaagagcctg attcactcac tccggaattg ctggcgactc ttctccttcg
     5461 acttggcgca acggaccagt ccaactccga tgatatcctc tggaatcatg agcctcatct
     5521 tgcactgaga gacggcgcgt tctacgtacc cagagtgctg cgcctagaca gcttgaaccg
     5581 ccgcgctagt gcgaagaacc gaaagctcac ccagagtacc agtcttgagc tctctgatac
     5641 tggcgtcgag atgtctcaac aagacggagc cttggagctg ttcgattacc ctgtcggcga
     5701 ttgtaggcca agcgagattc gtgtacaggt catggcatca tccatccatg cttttacact
     5761 cgatcgcccg gcgcccgttt acttctggat tggtcacgat gtccattcta aagacagagt
     5821 catgggcttt tcggaattca accgctccat aatcacggct acggaagatc agatctttta
     5881 ccgtggagac aatgtcagca cacaagacaa gcgggattca gatgctgagc agctgcaccg
     5941 attcatcgta caagcgatgg ctgcatctct tctcggtaat tttgaggcac ccatctgggt
     6001 acacggcgct ccaaaccact tcaagccggc cattactcgt gctgcccacg aagccaatgt
     6061 ctcagtgtcc ctgacgacat cagatattgc aagcgctcaa gacgagggtt tcatccaccc
     6121 gtacatcagc aagcgagatc ttcagtcact tgtcccaaga tgcgtcaaaa cactgatcaa
     6181 cctggaggcc gcgcaaaacg aggatctctc gaagctaatg cgagattccc tgccttcttc
     6241 taccaaggta atccagggtg atgatctgac gattccgatc aggtgcgaga agctgcgcga
     6301 gctagcccag gttattggtc atgacgaaat caagggcgca agtcacgcag accatgtcac
     6361 atccattgga gagctgacag aagacaaagt caaagcattg agccaagggg ctgtcataga
     6421 ctggcgcact accgatagcg tcaccacgac tgttcgacct ctggatcaaa gcgggctctt
     6481 cgatgccaac aagacctaca tccttttcgg catgacaggg gacgtcggca tatctgttgc
     6541 tgcatggatg gttgaccacg gcgctcgaaa cgtagtgctg ggtagccgaa accctagggt
     6601 accagagggc gtcattgaat acatggctcg aaaaggagcc aacctgtgcg cagtcaaggt
     6661 cgacgtgacc aagaaggaag agctgcgaga agcgtgtgcc aacatcaagg caaccatgcc
     6721 gccgattgcg ggtgtcatca atggcgccat ggttctccga gacagacttt tcgcccacat
     6781 gtcctgggac gactttgaag ctgtcctggc gccaaaggtc gctggaactc agaacctgga
     6841 cgagattttc agcaacgaac aagatgcgct ggatttcttc attgttcttt cgtcggcaac
     6901 atcgctggta ggcatcatag gccagtctgc ctacagcgca gcgaaccatt ttatgtcgag
     6961 tctggtgcgc cagcgacatg ctcgtggtct agctggttcc gttgtggcaa tcgggttcct
     7021 gacgggcttg ggctacattt tccgctcgga aaaggagcat ctcgatgcta ttgagagaca
     7081 agccgagaca gacctgcacg acatgctcgc ggaggcggtt gtgtgcggtc gacccaactc
     7141 tggtcagccg tctgagctca tcaccgccat caagacaaca ttcccggatg cgtggcacga
     7201 agacccacgc ctctcatgct accttgtaca ggatagcgtg gaagaaggca ctgatgccaa
     7261 agaagtcgag ggcaatgcca aggtcgaagc tcaattggca tcggccgagg atccaaagca
     7321 gtgcttgacg attctgataa agtgcttcag cgtggcgctg ggcaacatgc tccagctcga
     7381 cccggctcag atagcagtcg acgtccctgt tgtcgatcac ggcgttgact ctctggtttc
     7441 ggtccgtatc cgcgaatggt tcctcaaaga gctcggcgtc gacgtgccgg tactaaagct
     7501 catgtcgacc aaccactccc tgtcgcgcgt atgcgaggat gcactagcag gatggcggaa
     7561 gcttcgtgca gggcccccaa cagcgtccca gacagaggcc aaggacaacg agcctcgccg
     7621 cgacccagaa aaggactgga aaaaggaggt ggccgagctc attagcggaa tccgaggcct
     7681 catcccgcga ggcactgatg caatggcgag caccgagccg cctcggacaa acgctcgccg
     7741 ggtcgttgtt acaggatgta cagggttcct gggcactcac ctcctgcgcc atctggtcgc
     7801 cgatgcgagt gtcgctgagg tacattgtct gtgtatccgc tcacgccatg tacgcgtcca
     7861 ggacgccaaa atccgtgaat acgccggcga cctagccaag ccgctgttgg gactctctgc
     7921 taccgacttc aagcgcctca gtcagacagc agacgtgatc atccacctgg gtgccgatgt
     7981 caaccatctc aagtcgtacg aggcgatgcg tgctgccaat gtggtgtcga cacagacact
     8041 gcttgccatg gcgatgccgc gcagcgtccc cgtgcactac atctcatcct cgtcggtggc
     8101 catgctgcag aagggaactt cggaactcgc agaagtggcg ccctcgagca tctcaccgcc
     8161 acgtgacgtc gattcgttga tgaagagcgc cgtgggttac gccgccagca aatgggtggg
     8221 tgggatgctg cttgaaaacg tcgagggccc tccaacggtg gtgcatcgct ttcccaacat
     8281 catgggccat gatgcccctg aagagattcc cttggtagct ctcgatcgct actgcaccaa
     8341 gatgcgggcc gtaccagcgc tggatccccg cttgtggatc ggccagctcg atgtcatcga
     8401 tgttggccag gttgtcccgg actttgtgga aatggcgtgg agcctggatt cccgtgaatc
     8461 attcgccgta cacaactact gcagcaataa cagttacttg ctttcggatc ttgcgggaat
     8521 gtacagagag agactcgggg ccaatgttga ggttctgccg acgcctgagt ggatgcgcag
     8581 ggccatcgcg atgggtatgc ccaagggtgt tgaagccaca tttgtgggcc atgacgaagt
     8641 gtttgtgtcg cctgttttgc gcaagggtcc ggccaagaaa tagggagatt gataagactt
     8701 ttctagttgc atatctttta tatttaaatc ttatctatta gttaattttt tgtaatttat
     8761 ccttatatat agtctggtta ttctaaaata tcatttcagt atctaaaaat tcccctcttt
     8821 tttcagttat atcttaacag gcgacagtcc aaatgttgat ttatcccagt ccgattcatc
     8881 agggttgtga agcattttgt caatggtcga aatcacatca gtaatagtgc ctcttacttg
     8941 cctcatagaa tttctttctc ttaacgtcac cgtttggtct tttatagttt cgaaatctat
     9001 ggtgatacca aatggtgttc ccaattcatc gttacgggcg tattttttac caattgaagt
     9061 attggaatcg tcaattttaa agtatatctc tcttttacgt aaagcctgcg agatcctctt
     9121 aagtatagcg gggaagccat cgttattcga tattgtcgta acaaatactt tgatcggcgc
     9181 tatgcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     9241 gcgcttggcg agactcaaac aagatatatt ttgcccgcct tacagacgaa actagttgga
     9301 ggttatggag catactatca cgtgggcggc cactggtgag ttactacacc ccaggggcaa
     9361 cgttgatgct cctaaaaaac tctggctgga cgcaagccgt aacacccgtg tcacttcata
     9421 atcgtttgca attcagggct tgatctacac attaccctgt tatccctatt aattaatacg
     9481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10621 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg
    11341 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
    11401 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
    11461 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
    11521 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
    11581 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
    11641 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
    11701 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
    11761 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
    11821 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
    11881 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
    11941 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
    12001 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
    12061 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
    12121 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
    12181 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
    12241 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
    12301 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
    12361 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
    12421 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
    12481 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
    12541 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
    12601 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
    12661 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta
    12721 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgagcgcgtt tcggtgatga
    12781 cggtgaaaac ctcggcgcgc cattaccctg ttatccctaa tatacaacgc aaagaagctg
    12841 gaaaattggc gagggaatcc tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg
    12901 tcgtgctagc gtacagggtg cactttgtaa ccatttggga caccggacac tcgctgtttt
    12961 cgaaattacc ctttaagcgc gggtattgaa ccaggcttat gcccaagatc gtagcaagca
    13021 tttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca
    13081 ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc
    13141 gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact
    13201 gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag
    13261 agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat attatttaag
    13321 gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc
    13381 ttttacattt cagcaatata tatatatata tttcaaggat ataccattct aatgtctgcc
    13441 cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc
    13501 attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat
    13561 catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga tgaggcgctg
    13621 gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt
    13681 accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac
    13741 gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag
    13801 ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac
    13861 tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc
    13921 gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca
    13981 ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga
    14041 aaaactgtgg aggaaaccat caagaacgaa ttccctacat tgaaggttca acatcaattg
    14101 attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc
    14161 accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg
    14221 ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg
    14281 tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc
    14341 actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaag
    14401 gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt
    14461 ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt
    14521 gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat
    14581 aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata
    14641 cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa
    14701 gcaaattgat actaatggct caacgaaatg acggtatcga taagcttgat atcgaattcc
    14761 tgcagcccgg gggatccact agttctagat taattaatat ctaaaaattg ccttatgatc
    14821 cgtctctccg gttacagcct gtgtaactga ttaatcctgc ctttctaatc accattctaa
    14881 tgttttaatt aagggatttt gtcttcatta acggctttcg ctcataaaaa tgttatgacg
    14941 ttttgcccgc aggcgggaaa ccatccactt cacgagactg atctcctctg ccggaacacc
    15001 gggcatctcc aacttataag ttggagaa
//
