LOCUS       sequence-224104-       14563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU30.1-2.2
FEATURES             Location/Qualifiers
     source          1..14563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             528..551
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4145..4154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9011..9031)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9011..9029)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9368..9956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9448..9467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10127..10918,10919..10987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10750..10769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10988..11092)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11119..12461
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12082..12129)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12502..12520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12815..13222
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13223..14317
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
       61 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
      121 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
      181 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
      241 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
      301 aaggcagagg agagcataga aatggggttc actttttggt aaagctatag catgcctatc
      361 acatataaat agagtgccag tagcgacttt tttcacactc gaaatactct tactactgct
      421 ctcttgttgt ttttatcact tcttgtttct tcttggtaaa tagaatatca agctacaaaa
      481 agcatacaat caactatcaa ctattaacta tatcgtaata cacaatggat tataaggatg
      541 atgatgataa gactagtatg tcgaatcatg gacctgacga ggccatcgcc atcgttggca
      601 ttgcttgcaa gcttccacaa gaagcctcga ccgtcgaacg cttttggcaa ttcctcctgc
      661 aaggacgaag cgcacattca ccatggccag atgatcgctt cggagccggc cactatcatc
      721 ccgacaatga gcgagtcgga acacactcgg tcaaaggggc gcatttcttg gccgagccgc
      781 ccgaatattt cgatgcaccc tttttcggca tcaccaaagg cgaagccatt tgcatggacc
      841 ctcagcaacg tctggtgttg gagaacgtct accatgctct agagaatgca ggaatcccac
      901 tctccgaagc caatggatcc aacacgtccg tgtatgtgtc gggcttcaat cacgactatc
      961 tctccatcat aaatgcggat gcggagagcc ctttgcgata tcgtgcaacc ggattgacca
     1021 actccgtcct cagcaaccga gtctcttggt ttttcgattt caaaggtcca agcatgacca
     1081 tcgatactgc ctgtagcagc tccgtagtga cacttcatca ggcgtgccag agtctacgta
     1141 gtggcgagag cgacatggcc atatccagcg gggtcacggt cttggcttac cccaacgatg
     1201 tggcctccat gtcttatcaa ggattcctat cgcaagacgg ccggtgcttt agcttcgatc
     1261 accgtgccaa tggctatgct cgtggcgagg gagtcgggac gatcatttta aagcgtttca
     1321 gtgatgccat tcgagatggc aactctataa gagccgtcat tcggggcact ggtgtgaatc
     1381 aggacggccg aactcctgga ttgaccaacc cggatagtct ggcgcaggaa aggctgatca
     1441 ggacggtcta tgcaagggca ggcttgagtg tcaacaatac ggccatggtc gaggctcacg
     1501 ggactggtac cgcggcgggt gacccgatcg aagccaatgg aattgcgaga gccttctcag
     1561 ctcggccaag gaatctccct ccgctgtaca ttggggcttg taagtccggc attggtcatt
     1621 tggagggcgc ggcaggcgct gcagccatca tcaagtctgt tcttatcctt gagaatggga
     1681 tcatcccccc aaacgtcaat ttcgaaaaag tcaatccgaa gatcccggca acaaaatggc
     1741 ggcttcaatt ccctcttgaa ccaacgcctt ggcctacgat cggcttgcgc aggatctcta
     1801 tcaattcctt tggagtcggc ggtacaaacg gccatgttgt tctcgatgat gcttttcact
     1861 atctccagga ccgagggttg accgcgactc attatacacg acccttgcct ccgaccaacg
     1921 cagatctcga tctcgtaaac ggcgatagat tcaacatcac caatggacat gtgaatgggc
     1981 tcaccaacaa ccatcccaac agtgctggag atagcaatgc ccttgccgga gactggaggg
     2041 ctttcgatcc aatcgagtgt ccaccaaagc tcgttccctt ttctgctttc gatgaagaag
     2101 gctgcagtcg cgcagcaaaa acacatgcag aattcctcca tgccaggccc gaatcgatat
     2161 ccgactccga aagtttgacc tatctgaacg atcttgccca tacaatgagt cggcgctcga
     2221 agctggcctg gcggagtcac gtattagcaa ggtcgctggt cgagctcgag acacgtctgg
     2281 cttcgccctt gcccaaaccg ctgcgggctc gcaccactgt caacgtggcc tttgtgttca
     2341 cgggtcaggg agcacagtgg tatggcatgg gacgtgagct actctgctac ccggtgttcc
     2401 gtgagagtct ggctgcgtgc agagtttatt tgcagatcga atgtggctgc ccctgggatc
     2461 tcctcgacga gctcgccaaa gacaaagaac atagtcgtct tgatcaagca gccttggctc
     2521 agccggcgtg cactgccctt caagttgcga ttgtggattt gttgcaatcg tggaatatcc
     2581 agcccagtcg tgtcgtgggc cattcctccg gcgagattgc tgctgcttac tgcgctggcc
     2641 ttctgactcg cgagtcggcc tggcgagcgt cttatttccg tggactagtc accgggaggc
     2701 agacgaccga gacgaaaggc gctatgcttg ctgtcgggtt gggtcaagaa gcgctcgccc
     2761 cttatcttga ccgaattcat gcccaactct ctggggaatt agttgtggca tgtctcaaca
     2821 gtcctcgcag cagcaccgtc tcgggtgatg tgcataaagt cgaagctctg caacaagtct
     2881 tggaggctga agggctcttc gcgaggaagc tcaaggtccc aaatgcctat cactctgcgc
     2941 atatgcagga attcgcagac gaatacgtcc gtctggtggg tacgctccac gcgccgagcg
     3001 agccacctca gtcacctgtg gtcatgatct cgagcgtcac gggggaagca gtcgaaggcc
     3061 gttgtctctc catcgactat tgggccaaga atctcctaaa tccagtgcgt ttctccgatg
     3121 ccctggcagg cctttgcttt agccgcacaa cccaaacaca ggccaccctc cgtgcagatg
     3181 ctgctggagc caacgtcttt gcggatgttt tgattgagct tggccctcat ggagcattgc
     3241 aaggcgccat aagagatgtg ctcgcagctc atgccgaggg cgttgcggta gcattcctcc
     3301 cggcactgaa acgcaatgcc cctggggcag acgtattcct gaacgccata ggctaccttc
     3361 atgcccgcgg gtatccgatc aacgttgatg acgtgaataa cagcgctgat acgactggtg
     3421 gaagagggtc ccgcccattg cgtatacttc ctgaacttcc tggataccag ttcaaccatt
     3481 ccaacaagtt ttggtatgag agtcgcttaa gccggaacta tcgcctccgc aaagagatca
     3541 gacatgacct gttcggtgct ccagtacccg actgggacgc gatggctcct cgattccgca
     3601 actttctgag aatctctgag cagccatggc tacgagatca catcgttact gataacatca
     3661 tcttgcctgg cgtaggctac gtgattgcgg taatcgaagc agccagacag ctggctgatc
     3721 caaatgtaca attgagcggc ttccggcttc gagatgtcaa catgaaacgt gcattgatcg
     3781 tgcccgacac gaaagaaggg gtcgaagtaa tgttgaattt caagccagtt gatgagtcca
     3841 gtgtcggcac ttcggcttat tggcgtcggt ttcaagtagt gtcctataac ccaaccgcga
     3901 acgactggat cgaacactgc actggatacg ttggcgtgga tcatgagaca tctgccccca
     3961 atccggtcga ccagggacga gaagcccgac acgacgcaga acgctggcac gccgctttca
     4021 ctgctgcaga agagcagtgt caatctccct gggacttttc caaggcctat gagcgattgg
     4081 caagaatcgg gctgaacttt ggaccgctgt tccagaactg ctcggctgtc cggggtacct
     4141 ttgaccatgg tggccagact atgggcacag tcaccgtccc cgacgttgca agtgttatgc
     4201 ccaagggtat cgtatctccg cacttgatgc atccagcttg catggacagc ttgatgcact
     4261 ttgcgctggc cgcgatcatg gacgccacag gaaagagcaa cctcgaagtg gccatggtgc
     4321 cgcggttcat caaggacatt tggatttctg cggcaatgaa ccctgagcct ggtcatgtct
     4381 atcgtgctca cagccagacg cgcaagattg cgtttgagaa gttctcgaca gatgttgtcg
     4441 tctgggatgg acccactcaa gaaccccgta tctcctggag tggaattgaa gcaagccccc
     4501 tggattccgc cgcacagagc tcgctcggcg cagaaaagat gtgtcacgag attgaatgga
     4561 ttcctcacgt ggatatgtat ctcgaaggag gcgacgtcgc ttcgttcgct gagatgccca
     4621 agcctgattt ggactcagag caagctgcga tcgagatcga ttctcgtatg cagctggcct
     4681 cggtgcttct tgtcctggac gcactggagg aactagatgg caaggtgccg gccgctgctc
     4741 aaggtcatct ctatcggtat ttcgagtgga tgaagcacgt cgagagtgat ctccagctcg
     4801 gcaaggtgat cggtatccaa gcctctgagg ttgagcgact caaacatgac cacgctgcaa
     4861 aggtggagct atacaaacag gttgcttcgg acactgcgtt cggtgagttg gcagtacgaa
     4921 tgggcggcaa catcgtgccc gtcttaagag gtgagacaga cccgctgcag ctcatgttcg
     4981 ctgaggatgc catactcgat cgagtctaca gacgaactgc gaccttgaac gacctgccag
     5041 cgcaacagaa ggcctatttg aacgtattag ccaagaataa ggccaattta cgcgtgctcg
     5101 aggtgggtgc cggcactggc tcgtccactg cgcaagtact tgatgccctt gctcccatcg
     5161 cagatgatgg ggtgtccatt gctagtagcg tgtcacatta catgtacact gatatttcgg
     5221 ccgccttttt cgaacaagca aggcagaaat tccagccata cacgaacatc atggagtatc
     5281 gagttctgga cgcagaacaa gacatcgcca agcaggagag cttcgaagtc aggagctatg
     5341 atttgattgt agctcagaat gtcatccata ccaccaccga cctcaccaaa acactgagta
     5401 atcttcgcca gttgctgaag cccggaggcc gcctgcttct acaagaaggt ataaggcagg
     5461 attactactg gtccgggcta tcatttggcc agctgtcagg gtggtggagt ggcaccgaat
     5521 ccatgcgtca atggatgcca tgggtatcat ccaagcagtg gaatgaagtc ctcgaggctg
     5581 ctggttttac aggagcgcgc ttcgaacttc ccgatagcca caatgaacag ctccatagcc
     5641 agagtctttt cgtggcccag aacgcttctg tagacgccaa cgagcccggt cccatgtgga
     5701 agaacgttgt gctcgtcaca ctccctagct tcgagcgaag caacactcaa agcttgttgc
     5761 aagacatcaa agacaagttg aacgagaatc tccggttcca gaacatcagg atcgtatccc
     5821 tgctagaatt ggaagggatc gagtatagcc aaagtctctg cattgttcta gcagaactcg
     5881 aacgtcctgt gattgcagat ctgtcccagg ccgagtacga gggtgtccgc aaaatgctga
     5941 tcgagactaa aagcatcttg tgggtaactg gagatgagct tttctgcccc cagtacggag
     6001 ccataaccgg cctgatgcgt acaaacagat gggaacgtga cttggacgat tccaacctgg
     6061 tcacactgaa aatctctgag cctcgacctg aaaatacctc gctgagtgcg tcgattcgaa
     6121 ggctgtgtga ggcgcagttc tcgctcactc tcccagttga gaacatgaat ggcgagttca
     6181 tgcttcaaga tggccggatc tggacgagtc ggctccgcga ggccattcgt gcagatgaat
     6241 acttgcaaac agctctatac cgctcagaag cagtcccaac gctggccagg gatgctggca
     6301 ggcccatcaa gctcacgaca actgttccag gcctgctaga aaaacttgag tgggtgaccg
     6361 acgagacgta caatgagcca ctctccgata cagaagtaga aatcgagata cgagctgttg
     6421 gcctcaactt tcgcgatctc atgattgcca tgggggagca tacggcgtac tcgatgggca
     6481 gtgaagcagc tggcatcatc tctcgcgtgg gctccaaagt agtcgacttc gctcctggcg
     6541 acagggttgt ctacatctgt ggcctagacc atgtcgggtg catgcacacc tatggtcgtt
     6601 tggatcaatc gacagtcgcc aaggtccccg atgggctcag tttggagatg gctgccggct
     6661 tgcctgttgt ctatgcgaca gtcatttaca gcctacggga gacggctcgg ttgatgcccg
     6721 acgaaaccat tcttatccat gctgctgccg gtggggtggg ccaggctgcg attcgttacg
     6781 ctcaacatat tggcgccgag gtgtatgtaa ccgtatcgac tacgttgaag cgagacttga
     6841 taattaggga gttcgggatt ccagacgatc acatcttttc aagccgcgac ctcacattcg
     6901 tcccgggcgt gttgcgtatg acaaaaggca gaggtgttga cgtagtcttg aattcgctct
     6961 ctggtgaggc gctgcgccgc tcatgggatc tggtggcacc cttcggtcgt ttcattgaga
     7021 ttgggaagaa agacgccatg atgaatggca aagtggatct tcggccctac ttgcgcaacg
     7081 tgaccatggc gagcgtcgac ctcgtttcta tgatgaagaa caaaccgttt ctcatcaaga
     7141 tgctgacaga ggaaacgatg cggctgtgga aagagggtgt ggcactgcct gcatcaccaa
     7201 ctacgataat gcccatgtcg caggtagtgg atgcacttcg cattctccag gccggcacgg
     7261 gaatgggcaa gattgttctg gtgcctcggg aggatgacgt tctgccactc cttccaccgt
     7321 caccggcgcc gcttggcttt tgtcgagatg catcctacgt gctcagtggt ggccttggag
     7381 gtattggacg gagcgttgct gcatggatgg ctttgcgagg cgctcgaaat ttgatcttcc
     7441 tgtccagcag cggccgcatc actccagccg tcacaacgat gcgcgagact ctcgaagcag
     7501 acggatgtcg tgtgcatatc ttcacatgcg atgtcagcga caaggaacag ctacgtctcg
     7561 ttctcgaaca atgcaagacc ctcccaccta taagaggagt cgtccaagca gcaatgaagc
     7621 taaaggacat catgctcgag aacatgtcgc acgaggaatt ccagctggct atcagaccca
     7681 agattcaagg ttcgtggaac ctacacgaac ttctgcccca ggatctggag catttcatca
     7741 tgctgtcttc agccacgggc gtcctcggca accgggctca ggccaactac gccgcaggaa
     7801 acaccttcca ggacgcgctg gcgtacttcc gtcggcagca aggtctagcg gccacgacga
     7861 tcgacgttgg cgcagtgcta gacgtcggat acgtggccga tcacgcggac cgcctcgcca
     7921 tgacaaaata cctaggcagt atgatgaaag tgctgcgcga agaagagttg cttacgctta
     7981 tcgagtacgg catgaacgct gctctgcagt cgcctgcaca gcttgttacg ggcttgactc
     8041 cgttagacaa gcatcgtgct cgaggcgtgc cgatgctcag ttacatgaac ttccctctct
     8101 tcacgcagct gcggcggctc aatacgcagc aagacggcgc agggacgacc gggggtgatg
     8161 gacctgacgt tgaagcgcgg cttcgagcag ctcggactct ggatgaggcg gctcagattg
     8221 tgacggaatc ggtcattgat aagttgtctt ccttgctctc aattgcggtc gaggatgtgg
     8281 atccggcgcg gaccatcagt gccaatggcg ttgactcgct tgttgcgcta gaactgagga
     8341 cgttcatggc gagaaaggtc aaggcggatg tgcctgtgtt ggaaatcatg ggctcgttga
     8401 gtcttgctca gctgtgtagg aaggtggcat cgacgagcaa agcggtcgag ttgctaatag
     8461 caggaggcaa ctaaggagat tgataagact tttctagttg catatctttt atatttaaat
     8521 cttatctatt agttaatttt ttgtaattta tccttatata tagtctggtt attctaaaat
     8581 atcatttcag tatctaaaaa ttcccctctt ttttcagtta tatcttaaca ggcgacagtc
     8641 caaatgttga tttatcccag tccgattcat cagggttgtg aagcattttg tcaatggtcg
     8701 aaatcacatc agtaatagtg cctcttactt gcctcataga atttctttct cttaacgtca
     8761 ccgtttggtc ttttatagtt tcgaaatcta tggtgatacc aaatggtgtt cccaattcat
     8821 cgttacgggc gtatttttta ccaattgaag tattggaatc gtcaatttta aagtatatct
     8881 ctcttttacg taaagcctgc gagatcctct taagtatagc ggggaagcca tcgttattcg
     8941 atattgtcgt aacaaatact ttgatcggcg ctatgcggcc gccaccgcgg tggagctcca
     9001 gcttttgttc cctttagtga gggttaattg cgcgcttggc gagactcaaa caagatatat
     9061 tttgcccgcc ttacagacga aactagttgg aggttatgga gcatactatc acgtgggcgg
     9121 ccactggtga gttactacac cccaggggca acgttgatgc tcctaaaaaa ctctggctgg
     9181 acgcaagccg taacacccgt gtcacttcat aatcgtttgc aattcagggc ttgatctaca
     9241 cattaccctg ttatccctat taattaatac ggttatccac agaatcaggg gataacgcag
     9301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11101 cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac
    11161 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
    11221 aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat
    11281 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
    11341 aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca
    11401 aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc
    11461 tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt
    11521 agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc
    11581 cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg
    11641 attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga
    11701 tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac
    11761 gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact
    11821 acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt
    11881 ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag
    11941 agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt
    12001 tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct
    12061 tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat
    12121 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
    12181 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
    12241 gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg
    12301 taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt
    12361 atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta
    12421 gtctcatcct tcaatgctat catttccttt gatattggat catactaaga aaccattatt
    12481 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    12541 ggtgatgacg gtgagcgcgt ttcggtgatg acggtgaaaa cctcggcgcg ccattaccct
    12601 gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc ctgtttctgt
    12661 ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt gcactttgta
    12721 accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg cgggtattga
    12781 accaggctta tgcccaagat cgtagcaagc atttaactgt gggaatactc aggtatcgta
    12841 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca
    12901 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
    12961 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag
    13021 agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc
    13081 acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta
    13141 gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat
    13201 atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga
    13261 ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
    13321 tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc
    13381 tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt
    13441 gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt
    13501 actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc
    13561 cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt
    13621 tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga
    13681 tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
    13741 ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct tggataaagc
    13801 taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga
    13861 attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa
    13921 gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc
    13981 cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc
    14041 tttgccagac aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga
    14101 tttgccaaag aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa
    14161 attgtcattg aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt
    14221 ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga
    14281 tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata
    14341 tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg
    14401 aatatgttca tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga
    14461 gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgaaat
    14521 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggg
//
