LOCUS       sequence-224105-       12046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU35.1-2.2
FEATURES             Location/Qualifiers
     source          1..12046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..28
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5971..5991)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5971..5989)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6328..6916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6408..6427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7087..7878,7879..7947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7710..7729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7948..8052)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8079..9421
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9042..9089)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9462..9480
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9775..10182
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10183..11277
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11524..11540)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11524..11540)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aatggattat aaggatgatg atgataagac tagtatgtcc gtacataccc caaccacgga
       61 ttcggaatcc tacgagggtt ttcgcaagat ggagattgtc tattttagca acgacttccc
      121 taaagatgac ttggttgacg tttttcgccg cctacacaac catagcaagg acaagcattt
      181 ccctgtcttg gcccaattca tacgtgaggc cacctgggct atcaaggacg aagttcgcca
      241 actaccaact gagctcaaac aactgattcc gccattcaat actctattgg cttgggcgga
      301 aaacagccag cttcgggaag gcctaatctg tggggctgtg gatggagtgt tgctcgtcgt
      361 cgctcagcta gcaacttaca ttggatatgt cgagagtcgc tctggagatt tgtttgactt
      421 ttcaattaca tctcttgctg gcctaggcac tggcctacta tcgtcgagtg ccgtctcatt
      481 gtcgcaaaca ttggccgact tgccactggc aggcgcagat gcagcccgtc tcgccttccg
      541 cctgggcatt catgtcttag acatgtcgga gaacctggaa gctcgtgatt tatcagaaag
      601 cccagattcg tgggcgtacg tggttcacaa tgttgacccg actacggcct acaaggagct
      661 agatgacatg cattctcgaa tggagatacc caatactggc aaaatattta tcagcgctgt
      721 tagccggaca tcagtcacag ttagcggacc accatcgagg ttaaaagcgt tgtttaataa
      781 gtcagagtta ttccgcgagt cgaaggtcgt tgagctgcca gtgtacggcg gcctctgcca
      841 cgcgccgcat atcttcggtc tggaccatgt acatagtatc gttcacggct gttcattaaa
      901 cgccgctatc acaaattctc cgccagttat tccagtttta tcgaccagca gcggtttgcc
      961 atatctggcc aagagttcca tggagatgtt tgaatgcgtc gtctcagagc tgctcacgca
     1021 aacaatctat tgggataatg ttgtcagtgg catagtcgaa cgagccaagt ctaacgttac
     1081 cgcggaagca gtgcttcact gcttcggcaa ctccactccc cttaacgacc tcaatatgac
     1141 cctgaagagc aatgttccag atctcgacgt ctcgatcaac agtcttatgt catggatctc
     1201 tcaggtttca ccaagagata ctacacccca cggcactgca cagtctaagc tggccattgt
     1261 cggtatggcc tgcagatttc caggaggtgc cacaaatact gagaagtttt gggagatcct
     1321 agagcaaggc cttgatgtct cacgtcgagt tccagctgac cgctttgaca tcgacacaca
     1381 ctacgaccca accggcaagc agcagaataa gaccatgaca cagtttggct gctttattga
     1441 tgagcctggt ttgtttgatg cgcctttctt caacatgtct cctcgtgaat ctcaggccgt
     1501 cgatccgcag atgcgtttgg ctcttgtaac ggcatacgag gcactggagc aagctgggta
     1561 cgtcggtaat cgcacagctg ctacacagtt gcaacgcatt ggaacatatt atggacaagc
     1621 cgccgacgac taccgtgagg ttaaccaagg ccaggatgta ggcacatact atattccagg
     1681 cggttgccgt gcgttcggcc ccggtcgtat caattacttt tttaaatttg cgggtccaag
     1741 ctacagcatt gatactgctt gctcttccgg acttgcagcg atcgaggtcg cttgtcaggc
     1801 tctttggaac ggcgatgtag atatggctgt cacgggcggt gttaatgtgc taactaaccc
     1861 ggatgttttc gctggccttt gcaagagcca tttcctaact aaaggtcaca acgcatgcaa
     1921 gacctgggat tctaccgctg acgggtactg tcgtgcagac ggcgttgggt ctgttgttat
     1981 taaaaaactc gaagatgctg aagcagacaa cgacaacatc ttgggcgtca ttctcggtgc
     2041 gggaaccaac cattctgccg aggccgtctc tattactcac cctcatgccg gccatcaggc
     2101 gtatctgtct cgtcaggtgc ttcgccaggc cggcgtggat ccattggatg tttcctatat
     2161 tgagctccat ggcactggta ctcaagctgg tgaccacgag gagatgcaag gcataatgga
     2221 agtgtatgct ccgctcatca agcgacgtag gaaggaccag cccctacaca ttggtgctgt
     2281 aaaagctaac gttggccatg gtgaatctgt tgctggcaca acggcattga tcaaggtgct
     2341 tatcatgctc caaaaaaaat ctatcccgcc acatatcgga atcaaaacgg agatgaaccc
     2401 cagattcccc cgagacttcg acaaacgcaa cctccacatc gacttggaaa tgacaccctg
     2461 gccgcaaaca tcgtctaaga agcgacttgc agttgttaat aactttggtg ccgctggcgg
     2521 gaacacgact atggttttag aggaggcacc aactcgagac atatccgagg ccgatccacg
     2581 caatactcat atcatcgcca tatccgccaa gactaaaggg tcattaacag gaaatattga
     2641 gcgcctgatc gcccaccttg atgcgaatcc agatatcaac cttgccgacc tttcctacac
     2701 aacgactgca cgtcgctccc atcacagcca tcgtgtagca attgccactt ctgatatagg
     2761 ccacctaaag aagcagctta cttcccgcct agaaaatatc gattcaatga agcctattgg
     2821 aaattctggt agtccaccag tagctttcgc ctttactggc cagggctctt cttataaatc
     2881 gatgaacctt gagttgtacc acgatgtacc aacattcaga gagcatattc agcatcttga
     2941 ttctctcgct cgacgccaag gctttccatc tattattccc gctctagatg gctctcatcc
     3001 taaggaccac ttgcactctc cggtagtcac ccagcttgcc ctggtatgca ccgagattgc
     3061 tttggcaaaa tactgggttt ctcttggaat caagccagac gtggtcatgg gtcacagtct
     3121 gggcgaatat gctgcgatgc acatagctgg tgtggttact gcaagcgaca ccatattcat
     3181 ggttgggcgt cgtgctcaaa tgctagaaga aaagtgcacg attagcagtc atagtatgat
     3241 ggcggtccgg gctactcttc agcaaataga ggaatgctta ggaacaaagc cgtatacgat
     3301 tgcttgtgtc aatggacctt ccgatactgt cctcagcggt actaaggaga gtacagatga
     3361 tgtcgctgtg gtgctcgagg cggccggtta ccgttgcttc aagttggacg tcgcgtttgc
     3421 tttccactca gagcagaccg atcctatttt ggatgacttc gaggagatat caaagactgg
     3481 agttgttttt caggaaccta aacttccttt catctcgcca ttgctcggaa aggttatttt
     3541 tgacagtaag acgctgaacg caaactacgt tcgccgagcc accagggagc ctgttgactt
     3601 cttgtctgct ttgagaaatg ctcaaaagat ttctaccatt agcgatgaaa ctgtctggat
     3661 cgaaattgga ccacatcctg tctgcactaa ctttgtcaag actaccatcc cgtccacgaa
     3721 gcttaccatc ccatcgatcc gtcgtggaga taacaactgg aaaaccatgg ctgaaagcat
     3781 ggctgctctg catcttgctg ggtttggggt cagttggaac gaattccacc gcccatttga
     3841 gcaaaggctg cgccttttag acttgccaac atacgcctgg aatgacaaga actactggtt
     3901 gcagtataat ggtgactggt gtctgactaa gggcaatact ttctacgatg ccgagaagga
     3961 ggctgccaga gcaaaatctg tactaagatc gctgcctgcg agcgggtttc tgacaacgac
     4021 cgtgcagcag atcatcgacg agtcttttag cgattttgct ggcacggtgg tgatgcagtc
     4081 tgatcttatg caagcggata tgctcgccgc tgctcatggg cacagcatga acaattgcgg
     4141 tgttgttaca tcttccatcc acgctgacct cgcctacacc ctcggcgact atatataccg
     4201 caagttctat cccaaagcca aggaagtcaa tgttaacttt gccaacttgg ttgtcgcaaa
     4261 aggccttgtt gcccaaaaca gcaacaagac tccccaattg ttccgtgtaa ctgctgtcac
     4321 tgccgacgtc cgctcaggcg tggtcgactt aacgtggcag aatgttgaca atgatggcta
     4381 tgcccacgag ccatttgcaa ccgccaacat aatctatggg gattcatctg aatggatttc
     4441 atcttgggcc ccattaggtc acctcatcca cggccgcatc gaagcgcttg agcgcctagc
     4501 tacagaggga aaagccaacc gtttctctcg caaaatggca tacacgatct ttgccagtaa
     4561 cctagtcaac tacgctgaga aataccgtgg catgcaatct gtggtgatgc atgaactaga
     4621 agggtttgct gacgtgcaac tgaccacaaa ggagagtgga gtctggacag ttccaccata
     4681 ctttatcgac agtgtggtgc acttggctgg ctttatcatg aactgctcgg atgccattga
     4741 tacaaagaat aactactgtg ttactcctgg ttggaactcg atgcgattcg cgaagcctct
     4801 gcttccagga gccaagtatc ggtcgtatgt caagatgatt cctacagtcg aggatccgac
     4861 tatttacaat ggcgacgtgt acatcatgca agatgatacc atcatgggtg tggttggtag
     4921 catacagttc cgcagatatc cgcgcatcct tcttagccgc tttttctcgc cgccagacaa
     4981 gatggccacc ttcgatgtca agccaaaggg gcaggtggct gcccccaaaa ctgcggtccc
     5041 agcacaagat gcaacaaaat ttgatgcaaa agtgtcactc ccgatacttg ctcgcggtgc
     5101 atccgaacct gacagtgacg acaagcaggg caaccctcct gcccctgcac tgcctgcccc
     5161 cgatgccgcc gccccatcta tcgttgaatc cgcggccgct tccggcacta caactgcaaa
     5221 ggcaatcatg ctcattgcca acgaggcagg cctagaactt tctgagatgt cagacgacgc
     5281 ccttttcgtt aatttgggaa ttgacagctt gatgtctcta gttatcgcag aaaagctcag
     5341 atcagagcta gaagtcaagg tcagcggcag cctcttcttg gactacgaga ctattggtga
     5401 tttacggcag tggttggatg agtattatag ctagggagat tgataagact tttctagttg
     5461 catatctttt atatttaaat cttatctatt agttaatttt ttgtaattta tccttatata
     5521 tagtctggtt attctaaaat atcatttcag tatctaaaaa ttcccctctt ttttcagtta
     5581 tatcttaaca ggcgacagtc caaatgttga tttatcccag tccgattcat cagggttgtg
     5641 aagcattttg tcaatggtcg aaatcacatc agtaatagtg cctcttactt gcctcataga
     5701 atttctttct cttaacgtca ccgtttggtc ttttatagtt tcgaaatcta tggtgatacc
     5761 aaatggtgtt cccaattcat cgttacgggc gtatttttta ccaattgaag tattggaatc
     5821 gtcaatttta aagtatatct ctcttttacg taaagcctgc gagatcctct taagtatagc
     5881 ggggaagcca tcgttattcg atattgtcgt aacaaatact ttgatcggcg ctatgcggcc
     5941 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     6001 gagactcaaa caagatatat tttgcccgcc ttacagacga aactagttgg aggttatgga
     6061 gcatactatc acgtgggcgg ccactggtga gttactacac cccaggggca acgttgatgc
     6121 tcctaaaaaa ctctggctgg acgcaagccg taacacccgt gtcacttcat aatcgtttgc
     6181 aattcagggc ttgatctaca cattaccctg ttatccctat taattaatac ggttatccac
     6241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8041 aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt
     8101 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     8161 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     8221 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     8281 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     8341 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     8401 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     8461 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     8521 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     8581 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     8641 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     8701 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     8761 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     8821 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     8881 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     8941 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     9001 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     9061 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
     9121 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
     9181 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
     9241 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
     9301 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
     9361 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
     9421 catactaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9481 cctttcgtct cgcgcgtttc ggtgatgacg gtgagcgcgt ttcggtgatg acggtgaaaa
     9541 cctcggcgcg ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg
     9601 cgagggaatc ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag
     9661 cgtacagggt gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac
     9721 cctttaagcg cgggtattga accaggctta tgcccaagat cgtagcaagc atttaactgt
     9781 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
     9841 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
     9901 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
     9961 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
    10021 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
    10081 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
    10141 tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag
    10201 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt
    10261 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt
    10321 ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc
    10381 aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt
    10441 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta
    10501 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt
    10561 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag
    10621 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa
    10681 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct
    10741 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg
    10801 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct
    10861 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac
    10921 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg
    10981 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca
    11041 tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg
    11101 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa
    11161 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac
    11221 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
    11281 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac
    11341 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta
    11401 catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga
    11461 tactaatggc tcaacgaaat gacggtatcg ataagcttga tatcgaattc ctgcagcccg
    11521 ggggatccac tagttctaga ttaattaata tctaaaaatt gccttatgat ccgtctctcc
    11581 ggttacagcc tgtgtaactg attaatcctg cctttctaat caccattcta atgttttaat
    11641 taagggattt tgtcttcatt aacggctttc gctcataaaa atgttatgac gttttgcccg
    11701 caggcgggaa accatccact tcacgagact gatctcctct gccggaacac cgggcatctc
    11761 caacttataa gttggagaaa taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa
    11821 aaaaaggcag aggagagcat agaaatgggg ttcacttttt ggtaaagcta tagcatgcct
    11881 atcacatata aatagagtgc cagtagcgac ttttttcaca ctcgaaatac tcttactact
    11941 gctctcttgt tgtttttatc acttcttgtt tcttcttggt aaatagaata tcaagctaca
    12001 aaaagcatac aatcaactat caactattaa ctatatcgta atacac
//
