LOCUS       sequence-224106-       13410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU34.2-2.2
FEATURES             Location/Qualifiers
     source          1..13410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1258..1557
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4106..4326
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4327..5130
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9296..9316)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9296..9314)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9653..10241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9733..9752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10412..11203,11204..11272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11035..11054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11273..11377)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11404..12746
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12367..12414)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12787..12805
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13147..13163)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13147..13163)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atttcagatt gagagaatga aaaaaaaaaa aaaaaaaaag gcagaggaga gcatagaaat
       61 ggggttcact ttttggtaaa gctatagcat gcctatcaca tataaataga gtgccagtag
      121 cgactttttt cacactcgaa atactcttac tactgctctc ttgttgtttt tatcacttct
      181 tgtttcttct tggtaaatag aatatcaagc tacaaaaagc atacaatcaa ctatcaacta
      241 ttaactatat cgtaatacac aatgacggac gataaagggg gctacaagca gatcaacaag
      301 tcgctcaaca tctgcgcttt cgaggactat ttgaaaggcc agaccgcgaa cctcgcccct
      361 atacctgatg tggagcaagt gacagaaagg gttttacgag tgcggggtca aaatccgggg
      421 aagttcacct tccaaggcac caataccttc ttagttggaa caggagcctc tcgcatattg
      481 atcgatacat ccggaggaga gcctgagtat gcgaagctgc ttgcatcaac ccttgagtct
      541 cggaacatta gcatcaagta tgttcttgta acacattggc atggagacca ctccgggggt
      601 gtaccggacc ttatcagaat gtatccgcac ttgaaagatc acatctacaa gaacgatccc
      661 gatccgggcc aacaagatat ccacgaaggg caagagtttt gtgtcgaggg ggctactgtt
      721 gtagcactgc actgcccggg gcattcagaa gatcacatgt gtttcatgct caaggaagag
      781 aactccatgt tcactggaga caacattctc ggtaccggga cgagcgcggt cgaagacttg
      841 ggcatattca tgtccagtct gcacaagatg atggatcaga aatgtcgagc tggccacccc
      901 gcccatggcg ttactattga tgatctaaac acaaaaatca gccgagagct atcgtcaaaa
      961 tacaggagag agaagcaagt catcagggcc ttgcgcaacc tccgcgatta cgggcaaaac
     1021 cgaccggtcg ttcgtgacat tgtcgaaaac atatacggaa actcggtgga tgagactacg
     1081 cgtacgttgg cgctggagcc gttcatcgac gaagttcttc gaaaacttgc tggtgatggt
     1141 aaagtgggtt tccaggttcg cgctggccag aaaaaatggt ttctaattgg taatgagcaa
     1201 tcaacggtgg gtgggatgcg gcaggtccag taccctgtca cacgcgccag cgcttaagcg
     1261 gatctcttat gtctttacga tttatagttt tcattatcaa gtatgcctat attagtatat
     1321 agcatcttta gatgacagtg ttcgaagttt cacgaataaa agataatatt ctactttttg
     1381 ctcccaccgc gtttgctagc acgagtgaac accatccctc gcctgtgagt tgtacccatt
     1441 cctctaaact gtagacatgg tagcttcagc agtgttcgtt atgtacggca tcctccaaca
     1501 aacagtcggt tatagtttgt cctgctcctc tgaatcgtct ccctcgatat ttctcatttt
     1561 ccttcgcatg ccagcattga aatgatcgaa gttcaatgat gaaacggtaa ttcttctgtc
     1621 atttactcat ctcatctcat caagttatat aattctatac ggatgtaatt tttcactttt
     1681 cgtcttgacg tccaccctat aatttcaatt attgaaccct cacgatccag ttctccagtg
     1741 acacagcctt tatctggtca aacctttctt tctaatcacc tatgctgatg cttaattaag
     1801 ggatttttgt ctccatcaac ggcatgcgcc caaaaatgac gtttttttta acccatagac
     1861 acgaaactac ccattttcca ccggcctgac ctaccaccgg aacaacggcc atctccaact
     1921 tgcaagttgg ggaaattaag agcatcgcag gtttaatgga agaaaaaaaa aaggtacagc
     1981 acagcgcaaa tggagttagt tcccttatgt cacacactca cacacagtcg gtcagatcaa
     2041 gcatactggg tgcgtataaa tagagtggcc attgccaccc tgtttatctc aaaatctgtc
     2101 ttgttagtgg tcttctccct ttttcaggtt acaattctct tgtttctact tagtatataa
     2161 gtatatcaag ctatattaag catactatca actgtcaact ctatcctcaa aatacaatac
     2221 aaaatggatt ccgctgaaca aagagagtgg ttctttcttg agaatttagg cctacgagtc
     2281 accctcatac ttttatctgg aatcaccgcc ttgattgttg attacagccg catgctctat
     2341 ttaagaaaaa agatgccacc tggcccatta ccctggccaa tagccggaaa cactctcagt
     2401 ctcccgaaag agaggccttg gtacatggtt gagcagctct caaaagacta caagtcccca
     2461 gtaattacgt tctggattgg acggttagtc ctcaatgaga tccttgtcaa acgctccaac
     2521 atctacaact ctagacgaca catgctcatg tttggtgagc tgatgaatga acaagataac
     2581 ctcatacaca aatacacata caccaacgag cagcgggaga gatttcgtgt cctgcgcaaa
     2641 atcacacatc atggtgttgg aacacaacaa gttcaacaat accgagaatt tcaagacgac
     2701 aagaacatgg tgctggcata cgatatccta aaaacacctg agctatttgc ttcacgtttt
     2761 gaacgctacg cggcgggcgt tgtctctatt gttggattgg gacgtcgtat ttccaacagt
     2821 caagatacgt tgattacgga agtcatcgct cagatgcaac atgccgcaca actcgctgtt
     2881 gtggcaaaag actggctacg gatcattgag acatttcctt ggttggtaaa gcttccccgt
     2941 agtatcgcac cctggttacg caatattaaa caatttagca aaacaaagta cggtcacgaa
     3001 aactacgcaa aattcctctt tcgcgaagcc ccacaataca accttagccg cggcgaagtc
     3061 tccagtcttg tctccaatct ttttggcgca ggcgcagata catccagtag cactctcatc
     3121 acagctgtaa ttgctatgcg cgccttccct gagacaatga aacctgcctg ggacgaactt
     3181 gatcgcgtag caggccactc aagaagccct acgcttgacg atgaattgcc ttatgtccgc
     3241 gcctttgcca aagttttccg ctggcgcgca gtagccaacc tcggaggaaa aactcatgca
     3301 ccgactcaag acgactactg gaactgctac ttcataccca agggaacttg gatacaaggg
     3361 aacgtatggg ctattcatca caacgagcgc gagtttccgg atcccgatcg atttaatcca
     3421 agacggcgcc tcgacacgga agatctgagg ccgtttccgg gagagaaggg atacatgacg
     3481 tttggatggg gaggaggagt tgtgctggtc aggcgcttgc tgagcagggc acgcatctca
     3541 gtgttgcgcg cttgctttgg gcctatcacg ttgagcctgc tatcgacgag aaaacaggaa
     3601 gtgagatacc agttgatatc ttcagctata ctaacggatc aaatcgacgt cctcagcctt
     3661 tctaggggag gaagcactga tgcgtctatc aaaatacgag aggccggcaa agtatatgct
     3721 tgaacaaatc gctcttaaat atatacctaa agaacattaa agctatatta taagcaaaga
     3781 tacgtaaatt ttgcttatat tattatacac atatcatatt tctatatttt taagatttgg
     3841 ttatataatg tacgtaatgc aaaggaaata aattttatac attattgaac agcgtccaag
     3901 taactacatt atgtgcacta atagtttagc gtcgtgaaga ctttattgtg tcgcgaaaag
     3961 taaaaatttt aaaaattaga gcaccttgaa cttgcgaaaa aggttctcat caactgttta
     4021 aaaggaggat atcaggtcct atttctgaca aacaatatac aaatttagtt tcaaagatga
     4081 atcagtgcgc gaaggacata actcattcaa ttcaattcat catttttttt ttattctttt
     4141 ttttgatttc ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
     4201 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
     4261 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
     4321 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
     4381 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
     4441 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
     4501 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
     4561 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
     4621 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
     4681 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
     4741 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
     4801 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
     4861 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
     4921 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
     4981 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt
     5041 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca
     5101 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata
     5161 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga
     5221 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg ataggaaaaa accgagcttc
     5281 ctttcatccg gcgcggctgt gttctacata tcactgaagc tccgggtatt ttaagttata
     5341 caagggaaag atgccggcta gactagcaag ttttaggctg cttaacatta tggataggcg
     5401 gataaagggc ccaaacagga ttgtaaagct tagacgcttc tggttggaca atggtacgtt
     5461 tgtgtattaa gtaaggcttg gctggggata gcaacattgg gcagagtata gaagaccaca
     5521 aaaaaaaggt atataagggc agagaagtct ttgtaatgtg tgtaacttct cttccatgtg
     5581 taatcagtat ttctacttac ttcttaaata tacagaagta agacagataa ccaacagcct
     5641 ttcccagata tacatatata tctttatttc agcttaaaca ataattatat ttgtttaact
     5701 caaaaataaa aaaaaaaaac caaactcacg caactaatta ttccataata aaataacaac
     5761 atgagtgcct tggagatatc tagcgaagta tgtgacgttc caaatggcac ccatgggacg
     5821 aagcgccgcc ggagttcgac tgtgctcaat ccaaggagtt atgtcaaaga gaagctttgg
     5881 acacaagctc cagtatttac gggtagcacc aactttgagc cacttgcaga tgcgaagaac
     5941 atcttgatca ctgggggcgc cggcttcata gcctgctggt tcgtacgcca tctagtcctt
     6001 acgtaccccc actataatgt ggtttccttg gacaaattgg actactgcgc aacactcaac
     6061 aacacgcgca ttctcgatag ccgaccgaac ttcacattcg agcagggcga catcacctcg
     6121 cctgctactg tgaagcgtgt gctccgtaga cacaaaaccg acaccattgt ccattttgcg
     6181 gcgcaatcgc atgttgacct cagctttggc aactcgtacg agttcacgca tacaaatgtt
     6241 tatggaacac atgtcttgct cgagagagcg agggaacatg gtgtcaataa attcatacac
     6301 atctcgacag atgaggtgta tggtgatgtg cctgttggcg cagcagacct gggcgaaacg
     6361 agcatactag cacccacaaa tccatactcg gccagcaaag cagcggcgga gatgatggtc
     6421 agcgcatacc ggagcagctt caagctaccc ctaattacgg tccgggcgaa caatgtatac
     6481 gggccacatc aatttcccga gaagattata cccaaattca tcatgctctt acagcgaaag
     6541 cgaaagctcc tcctccacgg cgacggctca ccaacgcggc gttacctcta tgcaggagat
     6601 attgtggatg ctctggacac cattttgcac aagggtgaca ttggacaaat ttacaacatt
     6661 gcctccaagg atgagatatc gaatacggag atctgctacc gtctccttga taatttcggc
     6721 atctcgcgag atacgacgag cgaattaaaa caatggattc agtataccga ggatcgtccc
     6781 ttcaacgacc agcggtacgc aacggatgga tcgaaacttg ctgcgctggg atggcagccc
     6841 aagacaagct tcgaagaagg ccttcgcata actgtggatt ggtatcgtag atttggcgaa
     6901 gtgtggtggg gcgatatcag ccgggttctg acgtcttttc ccgtggtaac aggaaatgaa
     6961 atcaggacga aggaggagca cgaagattta ccatcgtccg acggtgaggg gatacacgaa
     7021 gaagatagtg cagtatggac agtctagagt gcttttaact aagaattatt agtcttttct
     7081 gcttattttt tcatcatagt ttagaacact ttatattaac gaatagttta tgaatctatt
     7141 taggtttaaa aattgataca gttttataag ttactttttc aaagactcgt gctgtctatt
     7201 gcataatgca ctggaaggag aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc
     7261 agtttgaaaa ataactacat ggatgataag aaaacatgga gtacagtcac tttgagaacc
     7321 ttcaatcagc tggtaacgtc ttcgttaatt ggatactcaa aaaagatgga tagcatgaat
     7381 cacaagatgg aaggaaatgc gggccacgac cacagtgata tgcatatggg agatggagat
     7441 gatacctcca ttgggccgat gaagttagtc gacggataga agcggttgtc ccctttcccg
     7501 gcgagccggc agtcgggccg aggttcggat aaattttgta ttgtgttttg attctgtcat
     7561 gagtattact tatgttctct ttaggtaacc ccaggttaat caatcacagt ttcataccgg
     7621 ctagtattca aattatgact tttcttctgc agtgtcagcc ttacgacgat tatctatgag
     7681 ctttgaatat agtttgccgt gattcgtatc tttaattgga taataaaatg cgaaggatcg
     7741 atgaccctta ttattatttt tctacactgg ctaccgattt aactcatctt cttgaaagta
     7801 tataagtaac agtaaaatat accgtacttc tgctaatgtt atttgtccct tatttttctt
     7861 ttcttgtctt atgctatagt acctaagaat aacgactatt gttttgaact aaacaaagta
     7921 gtaaaagcac ataaaagaat taagaaaatg ccacacgtag aaagcacacc ctccacctac
     7981 atccccggcc gcctcgacgg caaagtcgcc ctcgtcactg gctccggccg cggcatcggc
     8041 gcagccgtag ccacgcatct gggccgtcta ggcgccaaag tggtcgtcaa ctacgccaac
     8101 tcgaccaaag atgctgaaaa agtcgtctca gagatcaaag cgctgggcag cgacgccatc
     8161 gccatcaaag ccgacatccg ccaagtctca gacattgtcc gcctcttcga cgaagccgtc
     8221 gcgcactttg gccacctcga catcgccgtc agcaactctg gcgtcgtaag cttcggccac
     8281 ttgaaagacg tgacagaaga agaattcgac cgcgtcttca gcctcaacac gcgcggccag
     8341 ttcttcgtcg cccgcgaggc ataccgccat ctgaccgagg gcggccgcat cattctcact
     8401 tcctcaaaca cggcccgcga cttcagcgtg cccgagcact cgctctactc cgggtccaag
     8461 ggcgcaatcg actcttttgt acgcatcttc tcaaaggact gtggcgataa gaagattacg
     8521 gttaatgcgg ttgctcccgg gggcacggtg acggacatgt ttcatgaggt ttcgcatcat
     8581 tatattccga atggccttga gtatacggct gagcagcgtc agcagatggc tgcgcatgcg
     8641 tcgccgttgc ataggaatgg gttcccgcaa gacgtggcca atgtggtggg atttttggtt
     8701 agtaaggagg gtgagtgggt gaacggcaag gtccttacgc tcgatggagg tgctgcataa
     8761 ggagattgat aagacttttc tagttgcata tcttttatat taaatcttat ctattagtta
     8821 attttttgta atttatcctt atatatagtc tggttattct aaaatatcat ttcagtatct
     8881 aaaaattccc ctcttttttc agttatatct taacaggcga cagtccaaat gttgatttat
     8941 cccagtccga ttcatcaggg ttgtgaagca ttttgtcaat ggtcgaaatc acatcagtaa
     9001 tagtgcctct tacttgcctc atagaatttc tttctcttaa cgtcaccgtt tggtctttta
     9061 tagtttcgaa atctatggtg ataccaaatg gtgttcccaa ttcatcgtta cgggcgtatt
     9121 ttttaccaat tgaagtattg gaatcgtcaa ttttaaagta tatctctctt ttacgtaaag
     9181 cctgcgagat cctcttaagt atagcgggga agccatcgtt attcgatatt gtcgtaacaa
     9241 atactttgat cggcgctatg cggccgccac cgcggtggag ctccagcttt tgttcccttt
     9301 agtgagggtt aattgcgcgc ttggcgagac tcaaacaaga tatattttgc ccgccttaca
     9361 gacgaaacta gttggaggtt atggagcata ctatcacgtg ggcggccact ggtgagttac
     9421 tacaccccag gggcaacgtt gatgctccta aaaaactctg gctggacgca agccgtaaca
     9481 cccgtgtcac ttcataatcg tttgcaattc agggcttgat ctacacatta ccctgttatc
     9541 cctattaatt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9841 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9961 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10021 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10081 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10141 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10201 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10261 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10321 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10381 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10441 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10501 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10561 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10621 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10681 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10741 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10801 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10861 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10921 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10981 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11041 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11101 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11161 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11221 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11281 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11341 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11401 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
    11461 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
    11521 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
    11581 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
    11641 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
    11701 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
    11761 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
    11821 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
    11881 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
    11941 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
    12001 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
    12061 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
    12121 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
    12181 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
    12241 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
    12301 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
    12361 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
    12421 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
    12481 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
    12541 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
    12601 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
    12661 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
    12721 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
    12781 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgag
    12841 cgcgtttcgg tgatgacggt gaaaacctcg gcgcgccatt accctgttat ccctaatata
    12901 caacgcaaag aagctggaaa attggcgagg gaatcctgtt tctgtctatc caagaatggg
    12961 catgaggtgg caaccgtcgt gctagcgtac agggtgcact ttgtaaccat ttgggacacc
    13021 ggacactcgc tgttttcgaa attacccttt aagcgcgggt attgaaccag gcttatgccc
    13081 aagatcgtag caagcattta aatgacggta tcgataagct tgatatcgaa ttcctgcagc
    13141 ccgggggatc cactagttct agattaatta atatctaaaa attgccttat gatccgtctc
    13201 tccggttaca gcctgtgtaa ctgattaatc ctgcctttct aatcaccatt ctaatgtttt
    13261 aattaaggga ttttgtcttc attaacggct ttcgctcata aaaatgttat gacgttttgc
    13321 ccgcaggcgg gaaaccatcc acttcacgag actgatctcc tctgccggaa caccgggcat
    13381 ctccaactta taagttggag aaataagaga
//
