LOCUS       sequence-224111-       12022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU34.1-2.2
FEATURES             Location/Qualifiers
     source          1..12022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(212..223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5944..5964)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5944..5962)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6301..6889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6381..6400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7060..7851,7852..7920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7683..7702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7921..8025)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8052..9394
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9015..9062)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9435..9453
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9748..10155
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10156..11250
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11497..11513)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11497..11513)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggattataag gatgatgatg ataagactag tatggaggtt gtatatttca gtaatgagtt
       61 ccctaaagat gatctctcgg agatcttcag acacttgcac aaccaaagca agaccaccga
      121 gcacgaaatt ctagcccgct tcctcagaga cgcaacaaga gcagtcaaga aagagattga
      181 aagactccca tcaactttga aaaaccagat ccctaccttc gatactctca tctcttgggt
      241 agagaagaag gagctccgcg agggtgaact atgtggcgcg gtcgatggag ttctgctgat
      301 actcgttcaa ctcgcggttt acatatttaa tgctgaaagc agtaatgatt caacattctc
      361 gcacgatgaa aagcacctca cgggtctggg cgtcggcttg ctttccgcta ccgcggttgc
      421 tctagctcga gacatttctg aactagcttt ctacggtgtg caggcgatca cagtcgcctt
      481 ccgcatggga gtccatgtac aaggcgtttc acattatcta gaagctcgcg acccggctga
      541 acccccaaag acatgggctt atgtggtcca taacgttgat ccccaagaga cgaaaaagga
      601 gctagaagca taccacgaag cagtgcccag ttcggatacc agtcgtatct tcatcagcgc
      661 catcagccga acatctgtca caatcagtgg cccaccttca aaacttgaaa atctcttcaa
      721 gaaggccaca gcattcagag acgccaagtc cattgcactg cctgtttatg gaggtctttg
      781 tcatgctccc cacatatacg actctcacga catcgaggct attgttcaag acgttgtcag
      841 ttcatcggat cgacttgttt cgcaaaacga accaagattt ccacttttct cgacaagctc
      901 cggtactcca tacatatcat ccagccccgc agaactgatc aagagcgttg tagcagagct
      961 cttgacaaag gctattgagt gggacaaagt tattgcacag gaggttgaat acgctaagca
     1021 gaacaaccga actcaaatca ggctgtcttg ctttggcaac tccattccat tgaatgatct
     1081 gcgttctgag ttgcaaaaga catccaagga tacttctatt tccttgaaca cctacatgtc
     1141 ggcgcttctc aaggatgcta ctgagaatgt aaccccccga ggtccagcac aatccaaact
     1201 tgccattgtg ggaatgtctt gcaggcttcc aggcggatca acggataccg agaaattctg
     1261 ggaagtgctt gaagcagggc ttgatgtgtc tcgccagata cctgcagacc gattcgacat
     1321 caatacgcac tatgaccctg aaggcaagga tctcaacaag acaatgacgc aatacggatg
     1381 cttcatcgaa gaaccgggaa tgttcgaccc aagtttcttc aacatgtcac ctcgagaggc
     1441 gatggttgtt gatccccaga tgcgactctc gcttgtcact gcgtatgagg cattggaacg
     1501 ggcaggctac gttgccaaca ggacccagtc gaccaggctc cagcgcatcg gaacatacta
     1561 cggccaagca gctgacgact atcgcgaggt aaaccaagga caggaagtcg gaacctatta
     1621 cattccagga ggatgccgtg cttttggacc tggtcgaatt aattacttct tcaaatttgc
     1681 cggtcccagc tacagcattg acaccgcctg ctcctcagga ctcgcggcta tcgaagtcgc
     1741 ttgtcaagct ctttggagtg gtgaagttga cactgctgta gcaggaggag tcaacatcct
     1801 tacgaacccc gatggtttta ctggtctaag taagggtcac ttcctaacca agggccacaa
     1861 cgcctgcaaa acttgggacg ctagtgctga tggctactgt cgcgctgatg gcgtgggatc
     1921 gattgtcatc aagaggctcg aagatgcaca agctgataag gataacattc ttggtgttat
     1981 ccttgctgca ggcaccaacc attcggcaga ggctgtctcc atcacccatc cccatgcagg
     2041 acatcaatca tatttgtcgc gccagatcat taggcaggca ggtattgatc cgctggacgt
     2101 ttcatatgta gagctccacg gtaccggcac tcaagctggt gattttgagg agatgaaggg
     2161 tgtcctagac gtgtatgctc caaacacctt tcccaaccgc aggtctgatc agaagctaca
     2221 cattggaagc gccaagtcaa atgtcggcca cggagaatca gtggctggga ctacagcact
     2281 tatcaaggtc ttgttgatgc tgcagaagaa tgccattcct ccacacatcg gcatcaaaac
     2341 agaggttaac ccacgcttac ctaaggatct ggatcagcga aatgtcgcaa tcccgttctc
     2401 catgaccgaa tggaagagtg atggcaagaa gaagcgtatc gcggctgtta acaacttcgg
     2461 agctgctggc ggcaacacta tgatgctttt ggaggaagca cctccagtcg tggtggcacg
     2521 cgagaaggaa accaggccaa cacatgtcat cgccgtgtct gcgaagacca agacctcttt
     2581 gcgcaacaac atcaaccgtc tccttgtgta tctcgataga aaccctgatg tctccatttc
     2641 ggatctcgcc tataccacga ctgcgcgcaa gtaccaccac tcgtacaggg tcgcagttag
     2701 tgctacggat cttgcttcag tgaagaagca actgtcaaca cagcttaaca aggttgattc
     2761 cttgaaaccc ttgaacaaaa ccaacccgcc gtcaatggca tttgttttca ctggccaggg
     2821 cgcctcgtac cagtcgatgg atctcgaact gtaccacgaa gcaccagtct tccgagagca
     2881 cattcaatat ctcgacagac ttgctcaagc ccaaggcttc ccttcattca tcccagcctt
     2941 ggatggctcc cacccacgcg accacgagca ccctcctgtc atcacccaac tagccctggt
     3001 ttgtctcgag attgcgttag cccactactg ggcttctttg ggcgtcaaac ctgatgttgt
     3061 catgggacac tccctcggcg agtatgcagc tatgcatgtc gctggagtca tcagtgcgaa
     3121 cgatgccatc tacatggttg gttgtcgggc actgatgctc cagaagtcat gcactccggg
     3181 cagccactgc atgatggctg ttcgcgcgtc tctcgagtct atcatcggcg catcgaatgg
     3241 caagccacac actgtggctt gtatcaatgg tcccactgac acggttctaa gcggcaccaa
     3301 ggcacagatg gatgacatcg ctgtagatct cgaggcagct gggttccgtt gcatcaagct
     3361 taacgtggca tttgcattcc actccgaaca ggtcgacccg atcctcgacg agttcgagac
     3421 gattagcaag agtggtgtta ttttccacga gcccaagctt cctgttgtct caccccttct
     3481 cggcaaggtc gtctttgacg gacggtcgct taacgccagc tacgtgcgaa atgctacacg
     3541 agcaacggtt gacttcctgt ccgctgcgaa gaacgcttgc gatgtgtcaa tcatcaataa
     3601 agagactgtt ttcatggaga tgggtcctca ccctgtctgt gtcggattca tcaagtccat
     3661 tttcccatcg acaaagaccg ccgtcccgtc cattcgccgt ggagacaaca attggaagac
     3721 catctccgag agcgcagcag ctattcatct cgatggcgtt gatgtggact ggaatgagct
     3781 ccaccgtcct tttgcggatg cagtgcgtct tcttgatctt cctacatatg cttgtgatga
     3841 gaagaactat tggatacaat acaatggcga ttggtgcttg accaagggca ataccttcta
     3901 cgacaagaag gtcgaggcgg cagagcctgc agttggcaat accaagaaat atgagtcttt
     3961 gagctctttg gtacagaaca tcatttcagt agatgtgaat ggagaccagg gcactgtgat
     4021 catgcagtcg gacttaatgc agaaggactt tttggctgct gcccatggac actcgatgaa
     4081 cagctgtggt gttgtcacat cgtccatcca tgccgaaatc gcatacactc tgggagagta
     4141 tctctaccgc aagttctacc caaagacaaa gcgagtggac atgaacatga gcaacttgca
     4201 cgtaaccagt gccctgatcg ccaagaaaca ctccatggac actccccagt ccatccaggt
     4261 cactgccagt accaacgaca tcaagagtgg tactcttgac ttgacatggc agaatgtcga
     4321 agaagatggc agcctgtcgg agccattcgc aacagccaat ctcgagtatg gcaacgcaga
     4381 cgattggctg tcgagttgga gtcccatggc acatctagtc cagcaccgta tcgagtcttt
     4441 ggagcagctc gctgccgagg gcaaggccaa ccgcttctct cgcaacatgg cctacaccct
     4501 cttcgcaaag aacctcgtcg actacgcgga caagtaccgc ggcatgcaat ccgtcgtcat
     4561 gaacgatctc gaagccttcg ccgaagtgaa gctcagcgac aaagtcgaca acggaaccta
     4621 caccgtaccc ccctacttca tcgacagcgt cgcccatctc gccggcttca tcatgaattg
     4681 ctccgacacc atcgacacca caaataacta ctgcgtgacc tcgggctgga aaaccatgcg
     4741 cttcgccgag ccccttaccc ccggcgctcg ataccgctcc tacgtcaaaa tgaccccaac
     4801 caaggacgac ccctccgtct acctcggcga cgtcttcgtc ctcagcgcag cagacaactc
     4861 cattgtcggc atggtcggcg gcatccactt ccgccgcttc ccacgcgtcc tcctcaaccg
     4921 cttcttcacc gcgccccagc ccaccaaggg cagcgctcaa cccgccaaat ccgccgcacc
     4981 acccgctccc agcaagccct ccgcaccacc taaagtgcaa gcggttcagg cagcaccggt
     5041 cgtacccgtc gctcctagag aagacgacac ccagtcctcc gccagcgaca cgtccgaccg
     5101 cgataccccc tccgaagcct cagtcaacac atccgctacc agcgtcgatg tgtctgcttc
     5161 tgcgccccgc gctgctaaca ccactgttga caaagctatc gatcttatcg ccaaggaaac
     5221 gggtatcgac gctgctgagc tgaccgacga tgcagagttt gccaacttgg gtattgatag
     5281 tctcatgtcg ctggtgttgt ccgagaagtt ccagaacgag ctgagtgtca aggtgaatgg
     5341 aagtttgttt ttggattatc cgacgattgg ggatttgagg gagtggttgg agaagtatta
     5401 tgcatgagga gattgataag acttttctag ttgcatatct tttatattta aatcttatct
     5461 attagttaat tttttgtaat ttatccttat atatagtctg gttattctaa aatatcattt
     5521 cagtatctaa aaattcccct cttttttcag ttatatctta acaggcgaca gtccaaatgt
     5581 tgatttatcc cagtccgatt catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac
     5641 atcagtaata gtgcctctta cttgcctcat agaatttctt tctcttaacg tcaccgtttg
     5701 gtcttttata gtttcgaaat ctatggtgat accaaatggt gttcccaatt catcgttacg
     5761 ggcgtatttt ttaccaattg aagtattgga atcgtcaatt ttaaagtata tctctctttt
     5821 acgtaaagcc tgcgagatcc tcttaagtat agcggggaag ccatcgttat tcgatattgt
     5881 cgtaacaaat actttgatcg gcgctatgcg gccgccaccg cggtggagct ccagcttttg
     5941 ttccctttag tgagggttaa ttgcgcgctt ggcgagactc aaacaagata tattttgccc
     6001 gccttacaga cgaaactagt tggaggttat ggagcatact atcacgtggg cggccactgg
     6061 tgagttacta caccccaggg gcaacgttga tgctcctaaa aaactctggc tggacgcaag
     6121 ccgtaacacc cgtgtcactt cataatcgtt tgcaattcag ggcttgatct acacattacc
     6181 ctgttatccc tattaattaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8041 agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     8101 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     8161 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     8221 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     8281 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     8341 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     8401 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     8461 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     8521 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     8581 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     8641 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     8701 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     8761 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     8821 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     8881 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     8941 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt
     9001 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     9061 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     9121 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     9181 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     9241 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     9301 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     9361 ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga
     9421 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9481 acggtgagcg cgtttcggtg atgacggtga aaacctcggc gcgccattac cctgttatcc
     9541 ctaatataca acgcaaagaa gctggaaaat tggcgaggga atcctgtttc tgtctatcca
     9601 agaatgggca tgaggtggca accgtcgtgc tagcgtacag ggtgcacttt gtaaccattt
     9661 gggacaccgg acactcgctg ttttcgaaat taccctttaa gcgcgggtat tgaaccaggc
     9721 ttatgcccaa gatcgtagca agcatttaac tgtgggaata ctcaggtatc gtaagatgca
     9781 agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg
     9841 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc
     9901 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg
     9961 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac
    10021 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg
    10081 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatatttcaa
    10141 ggatatacca ttctaatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt
    10201 ggtcaagaaa tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc
    10261 aatgtcaagt tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt
    10321 gttccacttc cagatgaggc gctggaagcc tccaagaagg ctgatgccgt tttgttaggt
    10381 gctgtgggtg gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa
    10441 atccgtaaag aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct
    10501 cttttagact tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc
    10561 agagaattag tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc
    10621 gcttgggata gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct
    10681 ttcatggccc tacaacatga gccaccattg cctatttggt ccttggataa agctaatgtt
    10741 ttggcctctt caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgaattccct
    10801 acattgaagg ttcaacatca attgattgat tctgccgcca tgatcctagt taagaaccca
    10861 acccacctaa atggtattat aatcaccagc aacatgtttg gtgatatcat ctccgatgaa
    10921 gcctccgtta tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca
    10981 gacaagaaca ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca
    11041 aagaataagg tcaaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca
    11101 ttgaacttgc ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca
    11161 ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc
    11221 gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac
    11281 ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt
    11341 tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag
    11401 gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacga aatgacggta
    11461 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct agattaatta
    11521 atatctaaaa attgccttat gatccgtctc tccggttaca gcctgtgtaa ctgattaatc
    11581 ctgcctttct aatcaccatt ctaatgtttt aattaaggga ttttgtcttc attaacggct
    11641 ttcgctcata aaaatgttat gacgttttgc ccgcaggcgg gaaaccatcc acttcacgag
    11701 actgatctcc tctgccggaa caccgggcat ctccaactta taagttggag aaataagaga
    11761 atttcagatt gagagaatga aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
    11821 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
    11881 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
    11941 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
    12001 taactatatc gtaatacaca at
//
