LOCUS       sequence-224114-       15765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Prieto H, Miccono M, Aguirre C, Sanchez E, Castro A
  TITLE     Grape Biotechnology: Past, Present, and Future
  JOURNAL   In: Cantu D., Walker M. (eds) The Grape Genome. Compendium of Plant 
            Genomes. Springer, Cham.
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "In: Cantu 
            D., Walker M. (eds) The Grape Genome. Compendium of Plant Genomes. 
            Springer, Cham."
COMMENT     Sequence Label: pGMV-gSWEET4
FEATURES             Location/Qualifiers
     source          1..15765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    107..131
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1072..1417
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1360..1380
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1451..5554
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1451..5554
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5567..5587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5598..5846
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6318..6393
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6891..6966
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8816..8840
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10141..10770
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11199..12272
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12338..12532
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12768..12790)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12876..13016
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12931..12950
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13031..13048)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13202..13790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13282..13301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  13879..14646
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             complement(14654..15448)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 cgccttacaa cggctctccc ctgacgccgt cccggactga tgggctgcct gtatcgagtg
       61 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
      121 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
      181 agagctcaaa gtttaacgcg ttagcagaag gcatgttgtt gtgactccga ggggttgcct
      241 caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg gtcattttaa
      301 tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga caaggggggg
      361 cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc tttagcacga
      421 gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg ttcacactat
      481 aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtt
      541 ggatacgaat tattcgtacg accctcggta ccgatcggcg cgccagattt gccttttcaa
      601 tttcagaaag aatgctaacc cacagatggt tagagaggct tacgcagcag gtatcatcaa
      661 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga
      721 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa
      781 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt
      841 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa
      901 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag
      961 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
     1021 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     1081 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     1141 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     1201 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     1261 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     1321 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     1381 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcctg caggtagatc
     1441 gctcgtcgac atggataaga agtactctat cggactcgat atcggaacta actctgtggg
     1501 atgggctgtg atcaccgatg agtacaaggt gccatctaag aagttcaagg ttctcggaaa
     1561 caccgatagg cactctatca agaaaaacct tatcggtgct ctcctcttcg attctggtga
     1621 aactgctgag gctaccagac tcaagagaac cgctagaaga aggtacacca gaagaaagaa
     1681 caggatctgc tacctccaag agatcttctc taacgagatg gctaaagtgg atgattcatt
     1741 cttccacagg ctcgaagagt cattcctcgt ggaagaagat aagaagcacg agaggcaccc
     1801 tatcttcgga aacatcgttg atgaggtggc ataccacgag aagtacccta ctatctacca
     1861 cctcagaaag aagctcgttg attctactga taaggctgat ctcaggctca tctacctcgc
     1921 tctcgctcac atgatcaagt tcagaggaca cttcctcatc gagggtgatc tcaaccctga
     1981 taactctgat gtggataagt tgttcatcca gctcgtgcag acctacaacc agcttttcga
     2041 agagaaccct atcaacgctt caggtgtgga tgctaaggct atcctctctg ctaggctctc
     2101 taagtcaaga aggcttgaga acctcattgc tcagctccct ggtgagaaga agaacggact
     2161 tttcggaaac ttgatcgctc tctctctcgg actcacccct aacttcaagt ctaacttcga
     2221 tctcgctgag gatgcaaagc tccagctctc aaaggatacc tacgatgatg atctcgataa
     2281 cctcctcgct cagatcggag atcagtacgc tgatttgttc ctcgctgcta agaacctctc
     2341 tgatgctatc ctcctcagtg atatcctcag agtgaacacc gagatcacca aggctccact
     2401 ctcagcttct atgatcaaga gatacgatga gcaccaccag gatctcacac ttctcaaggc
     2461 tcttgttaga cagcagctcc cagagaagta caaagagatt ttcttcgatc agtctaagaa
     2521 cggatacgct ggttacatcg atggtggtgc atctcaagaa gagttctaca agttcatcaa
     2581 gcctatcctc gagaagatgg atggaaccga ggaactcctc gtgaagctca atagagagga
     2641 tcttctcaga aagcagagga ccttcgataa cggatctatc cctcatcaga tccacctcgg
     2701 agagttgcac gctatcctta gaaggcaaga ggatttctac ccattcctca aggataacag
     2761 ggaaaagatt gagaagattc tcaccttcag aatcccttac tacgtgggac ctctcgctag
     2821 aggaaactca agattcgctt ggatgaccag aaagtctgag gaaaccatca ccccttggaa
     2881 cttcgaagag gtggtggata agggtgctag tgctcagtct ttcatcgaga ggatgaccaa
     2941 cttcgataag aaccttccaa acgagaaggt gctccctaag cactctttgc tctacgagta
     3001 cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg accgagggaa tgaggaagcc
     3061 tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat ctcttgttca agaccaacag
     3121 aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag aaaatcgagt gcttcgattc
     3181 agttgagatt tctggtgttg aggataggtt caacgcatct ctcggaacct accacgatct
     3241 cctcaagatc attaaggata aggatttctt ggataacgag gaaaacgagg atatcttgga
     3301 ggatatcgtt cttaccctca ccctctttga agatagagag atgattgaag aaaggctcaa
     3361 gacctacgct catctcttcg atgataaggt gatgaagcag ttgaagagaa gaagatacac
     3421 tggttgggga aggctctcaa gaaagctcat taacggaatc agggataagc agtctggaaa
     3481 gacaatcctt gatttcctca agtctgatgg attcgctaac agaaacttca tgcagctcat
     3541 ccacgatgat tctctcacct ttaaagagga tatccagaag gctcaggttt caggacaggg
     3601 tgatagtctc catgagcata tcgctaacct cgctggatct cctgcaatca agaagggaat
     3661 cctccagact gtgaaggttg tggatgagtt ggtgaaggtg atgggaaggc ataagcctga
     3721 gaacatcgtg atcgaaatgg ctagagagaa ccagaccact cagaagggac agaagaactc
     3781 tagggaaagg atgaagagga tcgaggaagg tatcaaagag cttggatctc agatcctcaa
     3841 agagcaccct gttgagaaca ctcagctcca gaatgagaag ctctacctct actacctcca
     3901 gaacggaagg gatatgtatg tggatcaaga gttggatatc aacaggctct ctgattacga
     3961 tgttgatcat atcgtgccac agtcattctt gaaggatgat tctatcgata acaaggtgct
     4021 caccaggtct gataagaaca ggggtaagag tgataacgtg ccaagtgaag aggttgtgaa
     4081 gaaaatgaag aactattgga ggcagctcct caacgctaag ctcatcactc agagaaagtt
     4141 cgataacttg actaaggctg agaggggagg actctctgaa ttggataagg caggattcat
     4201 caagaggcag cttgtggaaa ccaggcagat cactaagcac gttgcacaga tcctcgattc
     4261 taggatgaac accaagtacg atgagaacga taagttgatc agggaagtga aggttatcac
     4321 cctcaagtca aagctcgtgt ctgatttcag aaaggatttc caattctaca aggtgaggga
     4381 aatcaacaac taccaccacg ctcacgatgc ttaccttaac gctgttgttg gaaccgctct
     4441 catcaagaag tatcctaagc tcgagtcaga gttcgtgtac ggtgattaca aggtgtacga
     4501 tgtgaggaag atgatcgcta agtctgagca agagatcgga aaggctaccg ctaagtattt
     4561 cttctactct aacatcatga atttcttcaa gaccgagatt accctcgcta acggtgagat
     4621 cagaaagagg ccactcatcg agacaaacgg tgaaacaggt gagatcgtgt gggataaggg
     4681 aagggatttc gctaccgtta gaaaggtgct ctctatgcca caggtgaaca tcgttaagaa
     4741 aaccgaggtg cagaccggtg gattctctaa agagtctatc ctccctaaga ggaactctga
     4801 taagctcatt gctaggaaga aggattggga ccctaagaaa tacggtggtt tcgattctcc
     4861 taccgtggct tactctgttc tcgttgtggc taaggttgag aagggaaaga gtaagaagct
     4921 caagtctgtt aaggaacttc tcggaatcac tatcatggaa aggtcatctt tcgagaagaa
     4981 cccaatcgat ttcctcgagg ctaagggata caaagaggtt aagaaggatc tcatcatcaa
     5041 gctcccaaag tactcactct tcgaactcga gaacggtaga aagaggatgc tcgcttctgc
     5101 tggtgagctt caaaagggaa acgagcttgc tctcccatct aagtacgtta actttcttta
     5161 cctcgcttct cactacgaga agttgaaggg atctccagaa gataacgagc agaagcaact
     5221 tttcgttgag cagcacaagc actacttgga tgagatcatc gagcagatct ctgagttctc
     5281 taaaagggtg atcctcgctg atgcaaacct cgataaggtg ttgtctgctt acaacaagca
     5341 cagagataag cctatcaggg aacaggcaga gaacatcatc catctcttca cccttaccaa
     5401 cctcggtgct cctgctgctt tcaagtactt cgatacaacc atcgatagga agagatacac
     5461 ctctaccaaa gaagtgctcg atgctaccct catccatcag tctatcactg gactctacga
     5521 gactaggatc gatctctcac agctcggtgg tgattcaagg gctgatccta agaagaagag
     5581 gaaggtttga ctcgagatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc
     5641 ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt
     5701 ctaatattaa atgaatgtaa gatcacatta taatgaataa acaaatgttt ctataatcca
     5761 ttgtgaatgt tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg
     5821 gactatggaa tatgattaaa gataaggagc tccggtgacg gacccatggg cttcgacttg
     5881 ccttccgcac aatacatcat ttcttcttag ctttttttct tcttcttcgt tcatacagtt
     5941 tttttttgtt tatcagctta cattttcttg aaccgtagct ttcgttttct tctttttaac
     6001 tttccattcg gagtttttgt atcttgtttc atagtttgtc ccaggattag aatgattagg
     6061 catcgaacct tcaagaattt gattgaataa aacatcttca ttcttaagat atgaagataa
     6121 tcttcaaaag gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga
     6181 acaatagtat ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat
     6241 cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt agtgattgga
     6301 ctggagcaga cacggctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     6361 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt tgcaaaattt tccagatcga
     6421 tttcttcttc ctctgttctt cggcgttcaa tttctggggt tttctcttcg ttttctgtaa
     6481 ctgaaaccta aaatttgacc taaaaaaaat ctcaaataat atgattcagt ggttttgtac
     6541 ttttcagtta gttgagtttt gcagttccga tgagataaac caatattaat ccaaactact
     6601 gcagcctgac agacaaatga ggatgcaaac aattttaaag tttatctaac gctagctgtt
     6661 ttgtttcttc tctctggtgc accaacgacg gcgttttctc aatcataaag aggcttgttt
     6721 tacttaaggc caataatgtt gatggatcga aagaagaggg cttttaataa acgagcccgt
     6781 ttaagctgta aacgatgtca aaaacatccc acatcgttca gttgaaaata gaagctctgt
     6841 ttatatattg gtagagtcga ctaagagatt gtatcagata gagtccagga gttttagagc
     6901 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     6961 cggtgctttt ttttgcaaaa ttttccagat cgatttcttc ttcctctgtt cttcggcgtt
     7021 caatttctgg ggttttctct tcgttttctg taactgaaac ctaaaatttg acctaaaaaa
     7081 aatctcaaat aatatgattc agtggttttg tacttttcag ttagttgagt tttgcagttc
     7141 cgatgagata aaccaataag cttgaccgag tgtacttcaa gtcagtggga aatcaataaa
     7201 atgattattt tatgaatata tttcattgtg caagtagata gaaattacat atgttacata
     7261 acacacgaaa taaacaaaaa aagacaatcc aaaaacaaac accccaaaaa aaataatcac
     7321 tttagataaa ctcgtatgag gagaggcacg ttcagtgact cgacgattcc cgagcaaaaa
     7381 aagtctcccc gtcacacatg tagtgggtga cgcaattatc tttaaagtaa tccttctgtt
     7441 gacttgtcat tgataacatc cagtcttcgt caggattgca aagaattata gaagggatcc
     7501 caccttttat tttcttcttt tttccatatt tagggttgac agtgaaatca gactggcaac
     7561 ctattaattg cttccacaat gggacgaact tgaaggggat gtcgtcgatg atattatagg
     7621 tggcgtgttc atcgtagttg gtgaaatcga tggtaccgtt ccaatagttg tgtcgtccga
     7681 gacttctagc ccaggtggtc tttccggtac gagttggtcc gcagatgtag aggctggggt
     7741 gtcggattcc attccttcca ttgtccttgt taaatcggcc atccattcaa ggtcagattg
     7801 agcttgttgg tatgagacag gatgtatgta agtataagcg tctatgctta catggtatag
     7861 atgggtttcc ctccaggagt gtagatcttc gtggcagcga agatctgatt ctgtgaaggg
     7921 cgacacatac ggttcaggtt gtggagggaa taatttgttg gctgaatatt ccagccattg
     7981 aagctttgtt gcccattcat gagggaattc ttccttgatc atgtcaagat attcctcctt
     8041 agacgttgca gtctggataa tagttctcca tcgtgcgtca gatttgcgag gagaaacctt
     8101 atgatctcgg aaatctcctc tggttttaat atctccgtcc tttgatatgt aatcaaggac
     8161 ttgtttagag tttctagctg gctggatatt agggtgattt ccttcaaaat cgaaaaaaga
     8221 aggatcccta atacaaggtt ttttatcaag ctggagaaga gcatgatagt gggtagtgcc
     8281 atcttgatga agctcagaag caacaccaag gaagaaaata agaaaaggtg tgagtttctc
     8341 ccagagaaac tggaataaat catctctttg agatgagcac ttgggatagg taaggaaaac
     8401 atatttagat tggagtctga agttcttact agcagaaggc atgttgttgt gactccgagg
     8461 ggttgcctca aactctatct tataaccggc gtggaggcat ggaggcaggg gtattttggt
     8521 cattttaata gatagtggaa aatgacgtgg aatttactta aagacgaagt ctttgcgaca
     8581 agggggggcc cacgccgaat ttaatattac cggcgtggcc cccccttatc gcgagtgctt
     8641 tagcacgagc ggtccagatt taaagtagaa aatttcccgc ccactagggt taaaggtgtt
     8701 cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt gtatcaggta
     8761 tttccgttgg atacgaatta ttcgtacgac cctcatagtt taaactatca gtgtttgaca
     8821 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     8881 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     8941 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     9001 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     9061 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     9121 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     9181 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     9241 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     9301 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     9361 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     9421 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     9481 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     9541 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     9601 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     9661 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     9721 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     9781 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     9841 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     9901 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg tgcggctgca
     9961 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
    10021 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
    10081 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
    10141 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
    10201 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
    10261 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
    10321 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
    10381 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
    10441 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
    10501 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
    10561 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
    10621 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
    10681 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
    10741 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
    10801 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
    10861 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
    10921 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
    10981 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
    11041 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
    11101 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
    11161 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
    11221 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
    11281 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
    11341 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
    11401 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
    11461 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
    11521 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
    11581 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
    11641 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
    11701 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
    11761 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
    11821 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
    11881 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
    11941 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
    12001 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
    12061 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
    12121 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
    12181 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
    12241 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
    12301 ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt ggatagcacg tacattggga
    12361 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
    12421 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
    12481 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
    12541 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
    12601 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
    12661 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
    12721 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    12781 cagctcccgg aaacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    12841 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
    12901 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    12961 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    13021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    13081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    13141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    13201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    13261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    13321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    13381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    13441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    13501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    13561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    13621 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    13681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    13741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    13801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    13861 tcatgcattc taggtactgg taatgactcc aacttattga tagtgtttta tgttcagata
    13921 atgcccgatg actttgtcat gcagctccac cgattttgag aacgacagcg acttccgtcc
    13981 cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct gcgtatatcg
    14041 cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt
    14101 catccatatc accacgtcaa agggtgacag caggctcata agacgcccca gcgtcgccat
    14161 agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa
    14221 cagccagcgc tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca
    14281 gacgatgacg tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt
    14341 aagtgacgta aaatcgtgtt gaggccaacg cccataatgc gggctgttgc ccggcatcca
    14401 acgccattca tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa
    14461 gtgaactgca gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg
    14521 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
    14581 acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
    14641 atcaccctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
    14701 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
    14761 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
    14821 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
    14881 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
    14941 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
    15001 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
    15061 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
    15121 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
    15181 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
    15241 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
    15301 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    15361 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    15421 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    15481 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    15541 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    15601 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
    15661 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
    15721 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgc
//
