LOCUS       sequence-224115-       11035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 11035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU17.1-2.2
FEATURES             Location/Qualifiers
     source          1..11035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      794..1093
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3624..3844
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3845..4648
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(6607..6627)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6607..6625)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6964..7552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7044..7063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7723..8514,8515..8583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8346..8365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8584..8688)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8715..10057
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9678..9725)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10098..10116
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10458..10474)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10458..10474)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgacatgaag accatcttgc ttccaataac tgtctttgcc tcggtagcag cccctgcgca
       61 gtcgccacaa ggcctcctac tcggagtctt ctggacatgg ttgcaccagc tccacgccaa
      121 cctctcaaac cagtacaagg cgctggagga agacaagatg aacaagccct ggcgaccctt
      181 gcccgcgcac cgcgtcactc cccggcaggc cgagctcctg cgctggaccc tgatcccact
      241 gacgatcggc ttctccacta tcggcggccc acacgccatc gccgccagta tctggctcgc
      301 aacagccttg gtcatctacg agcagccctc cgtcagcggc cactggctcg gcaagaacat
      361 cctcaactgc gccggctaca tcggtttcga atacggcgca acagcaatgc tgggcgaggg
      421 caggcctctg agcgcgtcgg cggtgctctt gctgctatgc agcggcctcg tcatcctcac
      481 caccatccac gcgcaggact tcgccgacat cgccggcgac tccgccctcg gccgcgtcac
      541 cttccccatc cgcgcccccg ccctctcccg cgcgttcatc tacctcggca tcccgctcct
      601 ctccctcgcg ctctccgtcg cctgggatat gttctgggcg gcccgcgtac tgttcctcgc
      661 gctcggctcc gcagtcgcgt ggcggttcta cgccttccgg acgatctcgc aggacgcgcg
      721 gtcgtatgtg ttgtacaatg tatggctatt cagtgtacac cttttgcctg ccacgcagcg
      781 gcactggatg tgagcggatc tcttatgtct ttacgattta tagttttcat tatcaagtat
      841 gcctatatta gtatatagca tctttagatg acagtgttcg aagtttcacg aataaaagat
      901 aatattctac tttttgctcc caccgcgttt gctagcacga gtgaacacca tccctcgcct
      961 gtgagttgta cccattcctc taaactgtag acatggtagc ttcagcagtg ttcgttatgt
     1021 acggcatcct ccaacaaaca gtcggttata gtttgtcctg ctcctctgaa tcgtctccct
     1081 cgatatttct cattttcctt cgcatgccag cattgaaatg atcgaagttc aatgatgaaa
     1141 cggtaattct tctgtcattt actcatctca tctcatcaag ttatataatt ctatacggat
     1201 gtaatttttc acttttcgtc ttgacgtcca ccctataatt tcaattattg aaccctcacg
     1261 atccagttct ccagtgacac agcctttatc tggtcaaacc tttctttcta atcacctatg
     1321 ctgatgctta attaagggat ttttgtctcc atcaacggca tgcgcccaaa aatgacgttt
     1381 tttttaaccc atagacacga aactacccat tttccaccgg cctgacctac caccggaaca
     1441 acggccatct ccaacttgca agttggggaa attaagagca tcgcaggttt aatggaagaa
     1501 aaaaaaaagg tacagcacag cgcaaatgga gttagttccc ttatgtcaca cactcacaca
     1561 cagtcggtca gatcaagcat actgggtgcg tataaataga gtggccattg ccaccctgtt
     1621 tatctcaaaa tctgtcttgt tagtggtctt ctcccttttt caggttacaa ttctcttgtt
     1681 tctacttagt atataagtat atcaagctat attaagcata ctatcaactg tcaactctat
     1741 cctcaaaata caatacaaaa tgtcgtatgc tcttttggca gcaggcgcgg cttgcatctt
     1801 gctcgtcggg aatggcctct acaccctgct gtggcatccc ctcgcctcat accctggccc
     1861 acgacttgcg gcgttgacga agtggtaccg cgcgtacttc gacctcgtcc acgatggcgg
     1921 ttggcttgag cacctggagg agcttcatcg tcagtatggc cctgtcgtac gcgtcgctgc
     1981 ttctgaacta cacttcgcgg agcccgcagc ataccatgac atccacaact tcacaaaccg
     2041 ttttgcaaag gaccccgagt tgtatcgtgc cttcgacctg ccacaggcgt ccttcggaca
     2101 ggtggatcct gtcgcggcga agacccgccg cgacaccttg ggaccactct tctcacgtca
     2161 aggtattcgc aagttcgagt tcgtcattca ggataaggtc gacctcctcg tcaaccgcct
     2221 catcgaggag ggctccaccc gccagcccgt caaccttttc ctcgccttcc gctccttcag
     2281 cttcgacacc atcatgtcct actgcttcgc cacctcctac cactccctcg agcacgaacg
     2341 cttcgcccac cccatgatcc ccatcatctt taaggtcaac gtcctcacct ggaccctgcg
     2401 ttacttcccc ttcctcatga gccttgtgcg ctacggtccg cgcttcatca cctcccggct
     2461 ctcgcccgta gccgttggct tccagggcct gttcgaccac tttgacaagc gggtggatga
     2521 ggtgttgagc gagccggaga gcctaggggc cgccgggcat gatacggtat tccatcatct
     2581 gatcaatggg aagagagaga ggccgagcaa ggaggtgctg atcgaggagg caatcacact
     2641 gtttggggcg gggagcgaga cgacggcggc ggcggtgaca acggcgattt tccatgttct
     2701 gaacgacaag cgcatccgcg agagggtgat ggaggagcta agggaggcat ggccagatcc
     2761 gacgatgcgg ttgtcggcgc aggagatcga gaagctgccg tatttgacag ctgtcatcaa
     2821 ggaaacgttg aggttcgcgc caggtgtcgt cactcctgcc cctcgcatcg tgcatcagga
     2881 ctccgtcatc gcgggccacc caattcctgc tggaacgacc gtggccatag gtgccacatt
     2941 cgtgcacctt aacccggaaa tctacgagga cccaaagagg ttcaagcccg agcgatggct
     3001 gaaggagggc gcgcaggcac tcgagagtgc atactttatc cccttttcga agggcccgag
     3061 gatgtgtctg gggcattacc ttgcgtggtg cgagatgtac ctcgtccttg cgaacgtcct
     3121 acgcaagacg gatatcactg tgttcgagac ggatgagacg gactttgagt tccgtcaata
     3181 ctggatgccc atcttccgag gacgacagct gaaggggttc gtcgagccga gaattgacta
     3241 aacaaatcgc tcttaaatat atacctaaag aacattaaag ctatattata agcaaagata
     3301 cgtaaatttt gcttatatta ttatacacat atcatatttc tatattttta agatttggtt
     3361 atataatgta cgtaatgcaa aggaaataaa ttttatacat tattgaacag cgtccaagta
     3421 actacattat gtgcactaat agtttagcgt cgtgaagact ttattgtgtc gcgaaaagta
     3481 aaaattttaa aaattagagc accttgaact tgcgaaaaag gttctcatca actgtttaaa
     3541 aggaggatat caggtcctat ttctgacaaa caatatacaa atttagtttc aaagatgaat
     3601 cagtgcgcga aggacataac tcattcaatt caattcatca tttttttttt attctttttt
     3661 ttgatttcgg tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg
     3721 aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg
     3781 aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata
     3841 aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc
     3901 caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg
     3961 taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa
     4021 aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc
     4081 attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa
     4141 tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac
     4201 gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga
     4261 agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct
     4321 atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt
     4381 tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat
     4441 tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac
     4501 cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc
     4561 aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata
     4621 tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact
     4681 aaactcacaa attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa
     4741 taccgcacag atgcgtaagg agaaaatacc gcatcaggat aggaaaaaac cgagcttcct
     4801 ttcatccggc gcggctgtgt tctacatatc actgaagctc cgggtatttt aagttataca
     4861 agggaaagat gccggctaga ctagcaagtt ttaggctgct taacattatg gataggcgga
     4921 taaagggccc aaacaggatt gtaaagctta gacgcttctg gttggacaat ggtacgtttg
     4981 tgtattaagt aaggcttggc tggggatagc aacattgggc agagtataga agaccacaaa
     5041 aaaaaggtat ataagggcag agaagtcttt gtaatgtgtg taacttctct tccatgtgta
     5101 atcagtattt ctacttactt cttaaatata cagaagtaag acagataacc aacagccttt
     5161 cccagatata catatatatc tttatttcag cttaaacaat aattatattt gtttaactca
     5221 aaaataaaaa aaaaaaacca aactcacgca actaattatt ccataataaa ataacaacat
     5281 ggcctccatc ctcaagtcga agtgcgtcct cgtcaccggc gcgaccgcgg gcatcgggcg
     5341 cgcgctcgct cgcgccatcc gtgacctccc ctcgaagccg actgtcatcg cgatgggccg
     5401 ccggcaggag cggctcgatg agcttgcgaa agagggcttc aagacggcca agttcgacat
     5461 gaacacgacg cccgacaaga tcagggcgtt cgtggatgat atcacggcgc agtacccgga
     5521 gctggacgcg gtcatcctga acgcgggcgt gcagtatatc ttcgacttca ccaagcccga
     5581 gagcgtggat ttcaacaaga tcagcaccga gttcaacgtc aactacctcg ccgtcgtgag
     5641 catgatgacg gccttcctcc cgcacctaat caagatcggg aaagagcgcc ccaccctcct
     5701 cgtcccggtg acctctggcc tcatgacgat ccccctaccg agcacggcga actactctgc
     5761 gacgaaggcg gccctccaca gcatctcctt ctccatccgc attcaaatgg agaacgccaa
     5821 gacaaacgtg cacgtcctcg agattatccc tcccctcgtc gagtccgagt tgcacgacaa
     5881 ccaaggcacc acggaggctc tgtccagatt ctggatgccc cttgccgact ttatcaacgt
     5941 cgtcctcgac ggcctcaagg agggcaagat cgccatcacc gccggcgcgg ccacctcagc
     6001 gtacctccag ttcgagaagg gcaaggagag ctaccgcaat gggctcatgg ggctgccagt
     6061 tacgcagtaa ggagattgat aagacttttc tagttgcata tcttttatat ttaaatctta
     6121 tctattagtt aattttttgt aatttatcct tatatatagt ctggttattc taaaatatca
     6181 tttcagtatc taaaaattcc cctctttttt cagttatatc ttaacaggcg acagtccaaa
     6241 tgttgattta tcccagtccg attcatcagg gttgtgaagc attttgtcaa tggtcgaaat
     6301 cacatcagta atagtgcctc ttacttgcct catagaattt ctttctctta acgtcaccgt
     6361 ttggtctttt atagtttcga aatctatggt gataccaaat ggtgttccca attcatcgtt
     6421 acgggcgtat tttttaccaa ttgaagtatt ggaatcgtca attttaaagt atatctctct
     6481 tttacgtaaa gcctgcgaga tcctcttaag tatagcgggg aagccatcgt tattcgatat
     6541 tgtcgtaaca aatactttga tcggcgctat gcggccgcca ccgcggtgga gctccagctt
     6601 ttgttccctt tagtgagggt taattgcgcg cttggcgaga ctcaaacaag atatattttg
     6661 cccgccttac agacgaaact agttggaggt tatggagcat actatcacgt gggcggccac
     6721 tggtgagtta ctacacccca ggggcaacgt tgatgctcct aaaaaactct ggctggacgc
     6781 aagccgtaac acccgtgtca cttcataatc gtttgcaatt cagggcttga tctacacatt
     6841 accctgttat ccctattaat taatacggtt atccacagaa tcaggggata acgcaggaaa
     6901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8701 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
     8761 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     8821 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
     8881 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     8941 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
     9001 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
     9061 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
     9121 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
     9181 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
     9241 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
     9301 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
     9361 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
     9421 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
     9481 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
     9541 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
     9601 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
     9661 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
     9721 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     9781 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     9841 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
     9901 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
     9961 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
    10021 catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca
    10081 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    10141 atgacggtga gcgcgtttcg gtgatgacgg tgaaaacctc ggcgcgccat taccctgtta
    10201 tccctaatat acaacgcaaa gaagctggaa aattggcgag ggaatcctgt ttctgtctat
    10261 ccaagaatgg gcatgaggtg gcaaccgtcg tgctagcgta cagggtgcac tttgtaacca
    10321 tttgggacac cggacactcg ctgttttcga aattaccctt taagcgcggg tattgaacca
    10381 ggcttatgcc caagatcgta gcaagcattt aaatgacggt atcgataagc ttgatatcga
    10441 attcctgcag cccgggggat ccactagttc tagattaatt aatatctaaa aattgcctta
    10501 tgatccgtct ctccggttac agcctgtgta actgattaat cctgcctttc taatcaccat
    10561 tctaatgttt taattaaggg attttgtctt cattaacggc tttcgctcat aaaaatgtta
    10621 tgacgttttg cccgcaggcg ggaaaccatc cacttcacga gactgatctc ctctgccgga
    10681 acaccgggca tctccaactt ataagttgga gaaataagag aatttcagat tgagagaatg
    10741 aaaaaaaaaa aaaaaaaaaa ggcagaggag agcatagaaa tggggttcac tttttggtaa
    10801 agctatagca tgcctatcac atataaatag agtgccagta gcgacttttt tcacactcga
    10861 aatactctta ctactgctct cttgttgttt ttatcacttc ttgtttcttc ttggtaaata
    10921 gaatatcaag ctacaaaaag catacaatca actatcaact attaactata tcgtaataca
    10981 caatgttcag ccttctccga cgacaccttt acaccctctt cctcttcacg tacag
//
