LOCUS       sequence-224127-        9226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human wild type ZFP64 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: LentiV_Neo_WT_ZFP64
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4198..4254
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4273..5067
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4327..4346)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4937..4956
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5083..5671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5136..5156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5554..5565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5743..5976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6054..6175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6091..6110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6145..6164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6215..6350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6370..6389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6371..6389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6399..6416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6399..6415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6408..6430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6557..7012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6644..6663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6854..6875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7038..7142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7143..7211,7212..8003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7361..7380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8174..8762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8663..8682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8916..8933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9050..9071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9086..9091,9092..9109,9110..9116)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9124..9140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9129..9151
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9148..9164
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9148..9164
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9183..9203
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9185..9203
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgaa cgcgagcagc
     2161 gagggcgaga gcttcgcggg ctcggtgcaa attccaggtg gcacaacggt gctggtggag
     2221 ctgactcccg acatccatat ctgcggcatc tgcaagcagc agtttaacaa cctggatgcc
     2281 tttgtagctc acaagcaaag tggctgccag ctgacaggca catccgcagc agcccccagc
     2341 acggtccagt ttgtatcgga ggaaacagtg cctgccaccc agactcagac caccaccaga
     2401 accatcacct cggagaccca gacaatcaca gtttcagctc cagaatttgt ttttgaacat
     2461 ggctatcaaa cttacctgcc cacggaaagt aatgaaaacc agacagccac tgtcatctct
     2521 ctccctgcca agtcacgcac caaaaagccc acaacaccac ctgctcagaa aaggcttaac
     2581 tgttgctatc caggttgcca attcaagact gcttatggca tgaaggacat ggagcggcat
     2641 ttaaaaattc acacgggaga caaaccccat aagtgtgaag tctgtggcaa gtgctttagc
     2701 cggaaagaca agctgaaaac tcacatgcgg tgccacacgg gcgtgaagcc ctacaagtgt
     2761 aagacgtgtg actacgccgc tgccgacagc agcagcctca acaagcacct gaggatccac
     2821 tcggacgagc ggcccttcaa atgccagatc tgcccctacg ccagccgcaa ctccagccag
     2881 ctcactgtcc acctgcgatc ccacacgggg gacgccccct tccagtgctg gctctgtagc
     2941 gccaagttca aaatcagctc ggacttgaaa aggcacatgc gggtgcactc gggggagaag
     3001 cctttcaagt gcgagttctg caatgtccgc tgcaccatga aggggaacct caagtcgcac
     3061 atccgtatca agcacagcgg gaataacttc aagtgtcctc attgcgactt cctgggtgac
     3121 agcaaagcca ccctccggaa gcacagccgc gtgcaccagt cggagcatcc tgagaagtgc
     3181 tcggaatgca gctactcctg ctccagcaag gccgccctgc gcatccacga gcgtatccac
     3241 tgcaccgacc gccctttcaa gtgcaactac tgcagcttcg acaccaaaca gcccagcaac
     3301 ctgagcaagc acatgaagaa gttccatggg gacatggtta agactgaggc tctagagagg
     3361 aaggacaccg gcaggcagag cagccggcag gtggccaagc tggatgccaa gaagagtttc
     3421 cactgcgata tatgcgatgc ctccttcatg cgggaggact cgctccgcag ccacaagaga
     3481 cagcacagtg agtacagtga gagtaagaac tcggacgtga ccgttctcca gtttcagatc
     3541 gaccccagca agcagcccgc cacgcccctc actgtgggac acctccaggt gcccctccag
     3601 cccagccaag tgccccagtt cagcgaggga agagtcaaaa tcatcgttgg gcatcaggtg
     3661 ccccaggcga acaccatcgt ccaggctgcc gccgctgcag tgaacatcgt cccgcctgcc
     3721 ttggtggccc agaacccaga ggaactccca gggaacagcc ggctgcagat cctgcgccag
     3781 gtcagtctga tcgccccccc tcagtcctcg cggtgtccga gcgaggcggg cgcaatgacc
     3841 cagccggctg tcctgctgac cacccacgag cagacggacg gagccactct gcaccagact
     3901 ctcatcccca cggcctcagg tggcccccag gaaggctctg gcaatcaaac tttcattacc
     3961 agttcgggta ttacttgcac tgactttgaa ggcctaaacg ccttgattca ggaggggaca
     4021 gcagaagtga cagtggtgag cgatggaggc cagaacatcg cagtggccac cacagcgcca
     4081 ccggtcttct cctcctcttc ccagcaagaa ctacccaagc agacctactc catcattcaa
     4141 ggggcagccc atccagcttt gctctgtccc gccgactcca ttccagatgg cagcggcgcc
     4201 accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg ccccatgacc
     4261 gagtacaagc ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4321 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4381 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4441 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4501 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4561 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4621 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4681 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4741 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4801 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4861 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4921 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4981 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5041 tatcgccttc ttgacgagtt cttctgaacg cgttaagtcg acaatcaacc tctggattac
     5101 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5161 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5221 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5281 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5341 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5401 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5461 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5521 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5581 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5641 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     5701 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5761 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     6001 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6181 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6241 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6301 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6361 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6541 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6961 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7381 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7681 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9001 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9061 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9121 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9181 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
