LOCUS       sequence-224152-       12279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHTV86.1-2.2
FEATURES             Location/Qualifiers
     source          1..12279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      912..1211
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3927..4147
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4148..4951
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(7902..7922)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7902..7920)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8259..8847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8339..8358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9018..9809,9810..9878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9641..9660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9879..9983)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10010..11352
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10973..11020)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11393..11411
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11753..11769)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11753..11769)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acccagaaca taaagtcgaa cccgcttctt ccgccgtctc cactttgttc tacattccat
       61 acctgatctg ggaatttacc gagtccaatt tcccaacctt tgtcattcca aacaccactt
      121 tcggtgtctt gggcgctttg gctggttctc acttggctga tggtcaatta ccatctttgt
      181 ggcaattgtc ccaagctttg cccttggtcg ttgccttcaa ttggtacaat gtcctgatct
      241 tcgatctggc caatcaaaga tccgaagaat ccgtcaaaga ggacctgatt aacaagccct
      301 ggaggccaat tccatccggt aaaattaccg gcgaacaaac tagaagatcc atgttggttg
      361 ccgtccccat cgccttggct ttgaattatt ggttgggtgt ctggaaagaa ggtgtcttca
      421 tccaaatctt gacttggtta tacaacgacc tgaagggcgg cgacgaagtt gtcagggatt
      481 tgatcatttc catcgcttac ggttgtttca accacggttc cttgagaatt gccttgggtt
      541 cccaagccac tgtcaatcaa gaaggtatga cctggatcct gatcattagc tctgtcatct
      601 tgaccactat gcaagttcag gatttgaagg accaagaggg cgataggact agaggtaggc
      661 tgtctattcc attgctgttg ggcgaaaagt tttccaggtt ctctatcgct atttctgccg
      721 ctgtttggtc ctgtgtctgt ccattttttt ggaagttgcc accattggcc tatgctggtc
      781 caatggttat tggtgctgtc ttgatgacct ccgttttgtt caggaagacc gttaaagccg
      841 acgatcaatc ttggaagatc tggtgcttgt ggactatgtc cttgtactct ttgccactgt
      901 ttactttgta agcggatctc ttatgtcttt acgatttata gttttcatta tcaagtatgc
      961 ctatattagt atatagcatc tttagatgac agtgttcgaa gtttcacgaa taaaagataa
     1021 tattctactt tttgctccca ccgcgtttgc tagcacgagt gaacaccatc cctcgcctgt
     1081 gagttgtacc cattcctcta aactgtagac atggtagctt cagcagtgtt cgttatgtac
     1141 ggcatcctcc aacaaacagt cggttatagt ttgtcctgct cctctgaatc gtctccctcg
     1201 atatttctca ttttccttcg catgccagca ttgaaatgat cgaagttcaa tgatgaaacg
     1261 gtaattcttc tgtcatttac tcatctcatc tcatcaagtt atataattct atacggatgt
     1321 aatttttcac ttttcgtctt gacgtccacc ctataatttc aattattgaa ccctcacgat
     1381 ccagttctcc agtgacacag cctttatctg gtcaaacctt tctttctaat cacctatgct
     1441 gatgcttaat taagggattt ttgtctccat caacggcatg cgcccaaaaa tgacgttttt
     1501 tttaacccat agacacgaaa ctacccattt tccaccggcc tgacctacca ccggaacaac
     1561 ggccatctcc aacttgcaag ttggggaaat taagagcatc gcaggtttaa tggaagaaaa
     1621 aaaaaaggta cagcacagcg caaatggagt tagttccctt atgtcacaca ctcacacaca
     1681 gtcggtcaga tcaagcatac tgggtgcgta taaatagagt ggccattgcc accctgttta
     1741 tctcaaaatc tgtcttgtta gtggtcttct ccctttttca ggttacaatt ctcttgtttc
     1801 tacttagtat ataagtatat caagctatat taagcatact atcaactgtc aactctatcc
     1861 tcaaaataca atacaaaatg gatttttact acgccaatca acaagctatc ttgactgccg
     1921 ttggtgcttt gtctggtatc atcgctcata ttactgtcta tatccgcggt gaatggcacg
     1981 tccaagcccc acaaattacc ttgcaattgt tcgctttgta cgttggcgtt ccagttttag
     2041 tcagaatttt gaaggacact gtcgttgccc aaatcctgca gggtgtcgtg ttttggtcct
     2101 acggctatat tctgggtttg atcgcctcta ttttgttgta taggattgtt ttgcatcccc
     2161 tgactagggc ctcgtttcag ggtccatggt atgccagatt cactaagatt tggcatgttt
     2221 gggctgcaag aggttccatg aaccataggg tcttggacga tttgcacaaa aagtatggca
     2281 acttcgtcag gactggtcca gctgaaatta cagtcttcca tccagatgtt ttcatggccg
     2341 tcgatggtcc cagatctgaa tgtatcaaag ccgaatggta cgacatttta catcccggtc
     2401 aagccttggt cactgctaga aataagtccg ttcatgctgc taggagaagg gaatggaatt
     2461 ctggcttcac tactaaagct ttgtccgaac acgaggctaa gatcctgaag tacgttgaca
     2521 agttggatca gtgtattgcc gaagacgcga agttggaaaa tgtcaccaat atgcgtgatt
     2581 tgctgttgtg gttcggcttc gatatcatgg gcgacttcgt cttctccaag tcttttggta
     2641 tgttggagaa caagcagtgg catcatatta ttgtcagact gcaaagggct ctgtctctac
     2701 taggtccatt ctcctccgtc ccatggttga tccaaattgg ttttaaactg gctccaaggg
     2761 tgtctgtctt gaaagattgg ttcgacactg ttgcctggtg tcaatcccaa atgagggata
     2821 gactggaaaa cggttctgct tcccaaagcc aagacttgtc ctactacttg atggaaaagg
     2881 attctgaagg caacggtgct actgagaacg aaaaatggca ctgggctgct ggtgattcat
     2941 tgttggctat tgtcgctggt tctgaaccaa cagcagttgc tttgattgct gttttttgtg
     3001 aactaggtag gcacccacag cacgctgagc aaattcatga agaggtcaag aacgtggacg
     3061 ttactgattt gaagactctg gtgtctttgc cccatttgaa tgccgtcatc aaagaatccc
     3121 tgaggttgca cccagctttg ttgaccggtg gtaataggaa aactaccgct tttgggttga
     3181 ccattgatga tcaatttatc ccacccaata ccactattgt ggcccccagg tacactattg
     3241 gtaggttgga gtcttgtttc gagaggccat ctgattttat ccctgaaaga tggactacta
     3301 ggcctgaaat ggtcagaaat ggtgctgctt acgctccatt cggtactggc catcactctt
     3361 gtctgggtaa aactttggct ctggatacta tgaaatacgt tctggctagg atcttgaaaa
     3421 actacaggtt cagggctgct ccaggtgaag attccttgtc tgccctggaa accgtcaggg
     3481 atcagtttac ttctaaccca ggcattctga aattgcagtt tgaaaagaga catactatcg
     3541 actaaacaaa cgctcttaaa tatataccta aagaacatta aagctatatt ataagcaaag
     3601 atacgtaaat tttgcttata ttattataca catatcatat ttctatattt ttaagatttg
     3661 gttatataat gtacgtaatg caaaggaaat aaattttata cattattgaa cagcgtccaa
     3721 gtaactacat tatgtgcact aatagtttag cgtcgtgaag actttattgt gtcgcgaaaa
     3781 gtaaaaattt taaaaattag agcaccttga acttgcgaaa aaggttctca tcaactgttt
     3841 aaaaggagga tatcaggtcc tatttctgac aaacaatata caaatttagt ttcaaagatg
     3901 aatcagtgcg cgaaggacat aactcattca attcaattca tcattttttt tttattcttt
     3961 tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
     4021 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
     4081 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     4141 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
     4201 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
     4261 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
     4321 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
     4381 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
     4441 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
     4501 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
     4561 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
     4621 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
     4681 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
     4741 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
     4801 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
     4861 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     4921 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     4981 actaaactca caaattagag cttcaattta attatatcag ttattaccct atgcggtgtg
     5041 aaataccgca cagatgcgta aggagaaaat accgcatcag gataggaaaa aaccgagctt
     5101 cctttcatcc ggcgcggctg tgttctacat atcactgaag ctccgggtat tttaagttat
     5161 acaagggaaa gatgccggct agactagcaa gttttaggct gcttaacatt atggataggc
     5221 ggataaaggg cccaaacagg attgtaaagc ttagacgctt ctggttggac aatggtacgt
     5281 ttgtgtatta agtaaggctt ggctggggat agcaacattg ggcagagtat agaagaccac
     5341 aaaaaaaagg tatataaggg cagagaagtc tttgtaatgt gtgtaacttc tcttccatgt
     5401 gtaatcagta tttctactta cttcttaaat atacagaagt aagacagata accaacagcc
     5461 tttcccagat atacatatat atctttattt cagcttaaac aataattata tttgtttaac
     5521 tcaaaaataa aaaaaaaaaa aaccaaactc acgcaactaa ttattccata ataaaataac
     5581 aacatggcct cttatgacta tattatcgtt ggtggtggct tggctggctt agttttggct
     5641 gctagattga ctgaagacag ttccaaaagg gttttggttt tggaagccgg tgaagatttg
     5701 actgctgatg ctagaacttc tgtccccgct atgtggccaa ctttgttgaa tactgacgct
     5761 gattggaaat tcagaacagc cgcccaggct ggtttcaaca atagggagat ttcattccca
     5821 caaggtaaat tgttgggcgg cagctctgcc ttgaatggtt tgtcctttac tccatcctcc
     5881 aaagctaacg ttgacgcttg ggctaagttg ggtaatccag gttgggattg ggcttctttc
     5941 tctaagtctt tagccaagtc ttatactctg acttccaagg ctggtactac tggttctggt
     6001 ccattgcaat tgtcctaccc aaacgacacc aacaactctt ggccaaaaat ttggcaacaa
     6061 accctgggtt ccttaggttt taggcaatcc ggtgaatcct acacacaaca atcttccggt
     6121 cccgttactg ttgccgattc tattcatcca gctactaaaa cccgctctta cgctggtaat
     6181 gcctatttgg aaccagctag gtctagggaa aacttgactg tcgttactgg agctgctgtt
     6241 tctaaaattg tcttcgcgaa agatggctct gctgttactg ctgaaggtgt tcagtacgtc
     6301 aaggatggcg aaactcaaac cgccaccggt aaagaaatca tcttgactgc aggcactatt
     6361 aattctccaa ggattctaga acactccggc gtcggtgatt ctaagttgct agccaaactg
     6421 ggtgtcgatg ttgttattga caaccccaat gttggtgaga atctgcaaaa ccacccaatg
     6481 gcaggtttgt ctttcgaggc cttagaagat gccgaaacca ttgatggttt atctagacaa
     6541 gatccagcag ctttgcaagc tgctatggct gcttacgctc aacaattggg tccatttgct
     6601 tcttctggca ctaataacgc tgctcaactg ccattcccag gtattcagac tgccgagggt
     6661 aaagctgaag ttgaggcttt gattgaaaag tacattactg gtgctaagaa cgtcacttcc
     6721 ccatttgaca aggcccaagc cgagttcgtc aggtctattt tgacttctcc agaggaagct
     6781 tctggttact acatctcctt ttctggcttc gcttctttta acccagatgg ttctatggct
     6841 ccaccacctc caggtactga aaaatacttc tctgtcgctt tgttgttgac tcatccattg
     6901 tccaggggct ccgtccacat taactctgcc tctgaaacct ctactgatgt tactatcgac
     6961 ccacagtact tctcccatcc actggacatt gaaatctttg ctagacactt gcgcttcgtt
     7021 gaatctttgg cctctgctga accattggct tctcatttga aggttggcgg taagagaaac
     7081 ccagctgctc ctccagctgg tattttttct gctgcatctg gtttggaaaa ggcgaaagaa
     7141 tatttgagac agactgctgt tggtgctcac cacttcactg gtacttgctc catgatgcca
     7201 aaagatttgg gtggcgttgt tgatgctgaa ttgagggttt atggctgtcc aaatttgagg
     7261 gtcgctgatg cctctattat cccattgact ccaagagcca atccacaagc tactgtttac
     7321 ggtgttgctg aacatgctgc taccattatt aaaggtggtt tgtaaggaga ttgataagac
     7381 ttttctagtt gcatatcttt tatatttaaa tcttatctat tagttaattt tttgtaattt
     7441 atccttatat atagtctggt tattctaaaa tatcatttca gtatctaaaa attcccctct
     7501 tttttcagtt atatcttaac aggcgacagt ccaaatgttg atttatccca gtccgattca
     7561 tcagggttgt gaagcatttt gtcaatggtc gaaatcacat cagtaatagt gcctcttact
     7621 tgcctcatag aatttctttc tcttaacgtc accgtttggt cttttatagt ttcgaaatct
     7681 atggtgatac caaatggtgt tcccaattca tcgttacggg cgtatttttt accaattgaa
     7741 gtattggaat cgtcaatttt aaagtatatc tctcttttac gtaaagcctg cgagatcctc
     7801 ttaagtatag cggggaagcc atcgttattc gatattgtcg taacaaatac tttgatcggc
     7861 gctatgcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     7921 gcgcgcttgg cgagactcaa acaagatata ttttgcccgc cttacagacg aaactagttg
     7981 gaggttatgg agcatactat cacgtgggcg gccactggtg agttactaca ccccaggggc
     8041 aacgttgatg ctcctaaaaa actctggctg gacgcaagcc gtaacacccg tgtcacttca
     8101 taatcgtttg caattcaggg cttgatctac acattaccct gttatcccta ttaattaata
     8161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8941 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9001 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9061 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9121 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9181 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9241 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9301 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9361 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9421 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9481 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9541 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9601 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9661 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9721 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9781 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9841 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9901 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9961 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc
    10021 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
    10081 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
    10141 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
    10201 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
    10261 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
    10321 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
    10381 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
    10441 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
    10501 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
    10561 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
    10621 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
    10681 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
    10741 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
    10801 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
    10861 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
    10921 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
    10981 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
    11041 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
    11101 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
    11161 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
    11221 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
    11281 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
    11341 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11401 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgagcgcg tttcggtgat
    11461 gacggtgaaa acctcggcgc gccattaccc tgttatccct aatatacaac gcaaagaagc
    11521 tggaaaattg gcgagggaat cctgtttctg tctatccaag aatgggcatg aggtggcaac
    11581 cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg gacaccggac actcgctgtt
    11641 ttcgaaatta ccctttaagc gcgggtattg aaccaggctt atgcccaaga tcgtagcaag
    11701 catttaaatg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
    11761 agttctagat taattaatat ctaaaaattg ccttatgatc cgtctctccg gttacagcct
    11821 gtgtaactga ttaatcctgc ctttctaatc accattctaa tgttttaatt aagggatttt
    11881 gtcttcatta acggctttcg ctcataaaaa tgttatgacg ttttgcccgc aggcgggaaa
    11941 ccatccactt cacgagactg atctcctctg ccggaacacc gggcatctcc aacttataag
    12001 ttggagaaat aagagaattt cagattgaga gaatgaaaaa aaaaaaaaaa aaaggcagag
    12061 gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat cacatataaa
    12121 tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc tctcttgttg
    12181 tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa aagcatacaa
    12241 tcaactatca actattaact atatcgtaat acacaatgc
//
