LOCUS       sequence-224155-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xflag tagged ZFP64 for flag ChIP experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: pHAGE_puro_ZFP64_N_3XFLAG
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2822..2887
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        4948..5447
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4983..5006)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5380..5399
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5468..6067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5468..5487)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5964..5984
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6081..6669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6134..6154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6552..6563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6744..6977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7096..7114)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7182..7286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7287..7355,7356..8147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7505..7524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8318..8906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8807..8826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9060..9077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9317..9338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9353..9358,9359..9376,9377..9383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9391..9407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9396..9418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9415..9431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9415..9431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9481..9500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtcaccat
     2821 ggattacaaa gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga
     2881 tgacaagatg aacgcgagca gcgagggcga gagcttcgcg ggctcggtgc aaattccagg
     2941 tggcacaacg gtgctggtgg agctgactcc cgacatccat atctgcggca tctgcaagca
     3001 gcagtttaac aacctggatg cctttgtagc tcacaagcaa agtggctgcc agctgacagg
     3061 cacatccgca gcagccccca gcacggtcca gtttgtatcg gaggaaacag tgcctgccac
     3121 ccagactcag accaccacca gaaccatcac ctcggagacc cagacaatca cagtttcagc
     3181 tccagaattt gtttttgaac atggctatca aacttacctg cccacggaaa gtaatgaaaa
     3241 ccagacagcc actgtcatct ctctccctgc caagtcacgc accaaaaagc ccacaacacc
     3301 acctgctcag aaaaggctta actgttgcta tccaggttgc caattcaaga ctgcttatgg
     3361 catgaaggac atggagcggc atttaaaaat tcacacggga gacaaacccc ataagtgtga
     3421 agtctgtggc aagtgcttta gccggaaaga caagctgaaa actcacatgc ggtgccacac
     3481 gggcgtgaag ccctacaagt gtaagacgtg tgactacgcc gctgccgaca gcagcagcct
     3541 caacaagcac ctgaggatcc actcggacga gcggcccttc aaatgccaga tctgccccta
     3601 cgccagccgc aactccagcc agctcactgt ccacctgcga tcccacacgg gggacgcccc
     3661 cttccagtgc tggctctgta gcgccaagtt caaaatcagc tcggacttga aaaggcacat
     3721 gcgggtgcac tcgggggaga agcctttcaa gtgcgagttc tgcaatgtcc gctgcaccat
     3781 gaaggggaac ctcaagtcgc acatccgtat caagcacagc gggaataact tcaagtgtcc
     3841 tcattgcgac ttcctgggtg acagcaaagc caccctccgg aagcacagcc gcgtgcacca
     3901 gtcggagcat cctgagaagt gctcggaatg cagctactcc tgctccagca aggccgccct
     3961 gcgcatccac gagcgtatcc actgcaccga ccgccctttc aagtgcaact actgcagctt
     4021 cgacaccaaa cagcccagca acctgagcaa gcacatgaag aagttccatg gggacatggt
     4081 taagactgag gctctagaga ggaaggacac cggcaggcag agcagccggc aggtggccaa
     4141 gctggatgcc aagaagagtt tccactgcga tatatgcgat gcctccttca tgcgggagga
     4201 ctcgctccgc agccacaaga gacagcacag tgagtacagt gagagtaaga actcggacgt
     4261 gaccgttctc cagtttcaga tcgaccccag caagcagccc gccacgcccc tcactgtggg
     4321 acacctccag gtgcccctcc agcccagcca agtgccccag ttcagcgagg gaagagtcaa
     4381 aatcatcgtt gggcatcagg tgccccaggc gaacaccatc gtccaggctg ccgccgctgc
     4441 agtgaacatc gtcccgcctg ccttggtggc ccagaaccca gaggaactcc cagggaacag
     4501 ccggctgcag atcctgcgcc aggtcagtct gatcgccccc cctcagtcct cgcggtgtcc
     4561 gagcgaggcg ggcgcaatga cccagccggc tgtcctgctg accacccacg agcagacgga
     4621 cggagccact ctgcaccaga ctctcatccc cacggcctca ggtggccccc aggaaggctc
     4681 tggcaatcaa actttcatta ccagttcggg tattacttgc actgactttg aaggcctaaa
     4741 cgccttgatt caggagggga cagcagaagt gacagtggtg agcgatggag gccagaacat
     4801 cgcagtggcc accacagcgc caccggtctt ctcctcctct tcccagcaag aactacccaa
     4861 gcagacctac tccatcattc aaggggcagc ccatccagct ttgctctgtc ccgccgactc
     4921 cattccagat tgatcgagaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     4981 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     5041 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     5101 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     5161 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     5221 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     5281 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     5341 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     5401 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     5461 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5521 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5581 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5641 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5701 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5761 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5821 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5881 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5941 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6001 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6061 tgcctgaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     6121 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6181 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6241 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6301 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6361 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6421 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6481 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6541 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6601 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6661 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6721 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6781 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6961 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7021 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     7081 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7141 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7201 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7261 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7321 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7381 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7441 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7501 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     7561 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7621 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7681 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7741 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7801 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7861 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7921 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7981 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8041 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8101 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9061 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9121 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     9181 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9241 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
     9301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9421 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
     9481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     9541 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     9601 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     9661 tttgttaaat atgtactaca aacttagtag t
//
