LOCUS       sequence-224160-        6295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express MLL/KMT2A promoter for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: pGL421_MLL_promoter
FEATURES             Location/Qualifiers
     source          1..6295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      857..866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             863..2512
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2516..2635
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2687..2808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2698..2717)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2752..2771
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3002..3359
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3002..3193
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3025..3045)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3210..3345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3272..3291
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3390..3989
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    4014..4062
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4218..4235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4389..4977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4469..4488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5177..5968,5969..6037))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6142..6190
                     /note="synthetic polyadenylation signal"
     misc_feature    6204..6295
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6244..6263
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatccgagtg cacagtagag
       61 cgaggaggtg gcaggagacc tgccactcgg tgtgtgcttt ttggaaagag ctccagcatg
      121 aaaatgggag gggctccttt tttatttttt aaagtgcgtt cattgagggc ctttctgttt
      181 tgcatatagt aaactgggca gcgcttctgt tttgcatgta gtacaataag ggctctttta
      241 ttctagaagc gttcaattcg ggctaaccca tcttgtatcc gtgccctggg gcggaggaga
      301 aggctcactg ctccccgacc cagccgccgc gttatactgg aagctgctct cccgggctgt
      361 tcgattccca gcgcgtcccg ggaacgtgtg taatcggcct ctcggcgctc aaggctgcag
      421 gcggcccggc tccctacgcc tccatcctcc gcgtagtcct cgccccctcc ccctctctcc
      481 ggaacccgcg ctctgctccg ggctcccggg cttccttccc ccctcctttt cccgagcaat
      541 gcctctcccg gagggcgggg catgcagtta tccaggttgc ggggccggcg ggcgggcagg
      601 aggcggcggc ccgggcctcg ggatggagtt gcagcgccca ggcgtcagag gcggaggccc
      661 gaggccgcta tacagattgc ggggctggcg ggcgcggccc ggcgctctgc atagcggccg
      721 gcagggtgca ggcggccggg cggcgcagag ctggttaggc aggttccggg gccccgtgcc
      781 cccccctccg cctccccgcc cccctgtgtt gtcgcctctc cctctcgaag cttggcaatc
      841 cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc
      901 attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta
      961 cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta
     1021 cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa
     1081 tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt
     1141 gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga
     1201 gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct
     1261 gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga
     1321 tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc
     1381 acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc
     1441 cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg
     1501 caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc
     1561 cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct
     1621 gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt
     1681 cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag
     1741 cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc
     1801 cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct
     1861 accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc
     1921 cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt
     1981 ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg
     2041 tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga
     2101 caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt
     2161 catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga
     2221 actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc
     2281 cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat
     2341 gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg
     2401 cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg
     2461 caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg tgaattctca
     2521 cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga gctgcgccca
     2581 ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca acgtctaagg
     2641 ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     2701 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2761 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2821 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2881 aacctctaca aatgtggtaa aatcgataag gatccgtttg cgtattgggc gctcttccgc
     2941 tgatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagctacct
     3001 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3361 agctcgattc ttctgacact agcgccacca tgaccgagta caagcctacc gtgcgcctgg
     3421 ccactcgcga tgatgtgccc cgcgccgtcc gcactctggc cgccgctttc gccgactacc
     3481 ccgctacccg gcacaccgtg gaccccgacc ggcacatcga gcgtgtgaca gagttgcagg
     3541 agctgttcct gacccgcgtc gggctggaca tcggcaaggt gtgggtagcc gacgacggcg
     3601 cggccgtggc cgtgtggact acccccgaga gcgttgaggc cggcgccgtg ttcgccgaga
     3661 tcggcccccg aatggccgag ctgagcggca gccgcctggc cgcccagcag caaatggagg
     3721 gcctgcttgc cccccatcgt cccaaggagc ctgcctggtt tctggccact gtaggagtga
     3781 gccccgacca ccagggcaag ggcttgggca gcgccgtcgt gttgcccggc gtagaggccg
     3841 ccgaacgcgc cggtgtgccc gcctttctcg aaacaagcgc accaagaaac cttccattct
     3901 acgagcgcct gggcttcacc gtgaccgccg atgtcgaggt gcccgaggga cctaggacct
     3961 ggtgtatgac acgaaaacct ggcgcctaat gatctagaac cggtcatggc cgcaataaaa
     4021 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgttcgaact agatgctgtc
     4081 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     4141 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     4201 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4261 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4321 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4381 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4441 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4501 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4561 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4621 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4681 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4741 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4801 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4861 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4921 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4981 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5041 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5101 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc
     5161 taaaccactg cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc
     5221 tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg
     5281 gcttaccatc aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg
     5341 atttgtcagc aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt
     5401 tgtccgcctc catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag
     5461 tgagtagttt ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt
     5521 tcggtatggc ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca
     5581 tattatgaag aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg
     5641 ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat
     5701 ccgtaagatg cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta
     5761 tacggcgacc aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca
     5821 gtactttgaa agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct
     5881 tgccgctatt gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat
     5941 cttttacttt caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga
     6001 agggaatgag tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt
     6061 gaagcattta tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac
     6121 gggaggtatt ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt
     6181 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
     6241 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
