LOCUS       sequence-224164-        3391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with constitutively active 
            rab38a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab38a-CA
FEATURES             Location/Qualifiers
     source          1..3391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(877..972)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1009..1028)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1010..1028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1033..1049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1033..1049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1151..1170)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1162..1971
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1229..1248)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2118..2706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2607..2626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2860..2877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3036..3063)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3155..3241)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3290..3312
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3304..3321
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3305..3321
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgctcg actggcgagt cacaagttgt gctttttccc tccgcggggc gtttctgttt
      181 ctcccagagt ctctcccagc ggatcacacg cgtccatgtt cgcctctgtc tcacacagcc
      241 atgcagaacc atcacctgaa ggaacatctc tataaaatcc tggtgatcgg ggacctggga
      301 gtggggaaaa ccagcattat caagcgctac gtgcaccaga acttctcacc aaactaccgt
      361 gccaccatcg gcgtggattt cgcgctcaaa gttctgaatc tcgaccagga gaccgttagg
      421 ctgcagctct gggacatcgc aggtctggag cgtttcggga acatgacgcg ggtctattac
      481 agagaagcaa tgggagccat cattgttttt gacgtcacgc ggccgtcgac gtttgaggcg
      541 gtgacgaagt ggaaggagga cttggatgca aagctgagtt tatccaacgg caaacaagtg
      601 gcggcggtgc tgctggccaa caaatgtgat cagggccgag acgtgctcac caacaacggc
      661 atcaagatgg agcagttctg ccaggagaac ggcttcgtgg gatggttcga aacttcagcc
      721 aaggagaata ttaatataga tgaagccgca cactgtctgg tgaagcacat tatagctaat
      781 gagaacgatc tgctgcagtc ggaggttgca gacactattt caccgcagca ggagtccagt
      841 cgtggaggaa cctgctcggc ctgtttcagg tcctgacaac tttattatac aaagttggca
      901 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      961 aaatcattat ttggagctcc atggtagcgt taacgcggcc gcgatatccc ctatagtgag
     1021 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1081 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1141 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1201 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1261 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1321 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1381 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1441 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1501 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1561 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1621 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1681 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1741 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1801 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1861 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1921 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     1981 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2041 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2881 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2941 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3001 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3061 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3121 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3181 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3241 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3301 cgttgtaaaa cgacggccag tcttaagctc gggccctgca gctctagagc tcgaattcta
     3361 caggtcacta ataccatcta agtagttggt t
//
