LOCUS       sequence-224165-        6264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MLL2/KMT2B promoter for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: pGL421_MLL2_promoter
FEATURES             Location/Qualifiers
     source          1..6264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             832..2481
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2485..2604
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2656..2777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2667..2686)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2721..2740
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2971..3328
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2971..3162
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2994..3014)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3179..3314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3241..3260
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3359..3958
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3983..4031
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4187..4204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4358..4946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4438..4457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5146..5937,5938..6006))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6111..6159
                     /note="synthetic polyadenylation signal"
     misc_feature    6173..6264
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6213..6232
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcccttcc actgcaatcc
       61 acggcgcgcg gagtctgatc tgggaacgct cggcccggga gagagaggaa cgcgtgctcc
      121 gaccctgctt tcggccaccc gcctcctctc cgggagcaca cggaccccga gaccgcggcc
      181 tctccggcgt cttccctcca caccgtgcca agccgccgcc aggtcgcgcc tggaacggag
      241 actgactatg ctttccaccg ctccttctcc agcccacgcc tcctgcagcc gcgaactgca
      301 tttcccaggg cctcgggggt cccggggccc gagcccgcgg caccctggga gtggtagtcc
      361 acgcgggccg ccggcctagc caagaacaat gggaggagcc tggggctagg accacacctc
      421 ccggagaccc ggcaacccgc tttccgggtc ctggccggga accccgccgt cccggccctc
      481 gcgccctggc tgccggcgga agaagcgagg cgtccggact acatttcccg gaggcccccg
      541 cggttcgccc ctctgcggcc gcgacgcggg ggtctgtgct ccccaggtgc ggtccgaggg
      601 cgccggccgg ccgcggactc cctctcccgt ggtgccctgc gcgtggccgg cccggccccc
      661 ggtctcccgc ggcggcgctg gttgttgtcg tgcgccgcgg ccgccccgcc ccgcccccgc
      721 tctctcccct ccccccggcc ctgcgcacgg gccgcccctc cccccgcctc cccggcccct
      781 ctcacggtgc caagataagc ttggcaatcc ggtactgttg gtaaagccac catggaagat
      841 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
      901 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
      961 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
     1021 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
     1081 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
     1141 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
     1201 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
     1261 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
     1321 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
     1381 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
     1441 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
     1501 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt
     1561 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg
     1621 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa
     1681 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag
     1741 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta
     1801 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca
     1861 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc
     1921 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt
     1981 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac
     2041 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc
     2101 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa
     2161 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac
     2221 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca
     2281 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc
     2341 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct
     2401 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag
     2461 aagggcggca agatcgccgt gaattctcac ggcttccctc ccgaggtgga ggagcaggcc
     2521 gccggcaccc tgcccatgag ctgcgcccag gagagcggca tggatagaca ccctgctgct
     2581 tgcgccagcg ccaggatcaa cgtctaaggc cgcgactcta gagtcggggc ggccggccgc
     2641 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2701 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     2761 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     2821 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     2881 atccgtttgc gtattgggcg ctcttccgct gatctgcgca gcaccatggc ctgaaataac
     2941 ctctgaaaga ggaacttggt tagctacctt ctgaggcgga aagaaccagc tgtggaatgt
     3001 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3061 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3241 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     3301 cttttttgga ggcctaggct tttgcaaaaa gctcgattct tctgacacta gcgccaccat
     3361 gaccgagtac aagcctaccg tgcgcctggc cactcgcgat gatgtgcccc gcgccgtccg
     3421 cactctggcc gccgctttcg ccgactaccc cgctacccgg cacaccgtgg accccgaccg
     3481 gcacatcgag cgtgtgacag agttgcagga gctgttcctg acccgcgtcg ggctggacat
     3541 cggcaaggtg tgggtagccg acgacggcgc ggccgtggcc gtgtggacta cccccgagag
     3601 cgttgaggcc ggcgccgtgt tcgccgagat cggcccccga atggccgagc tgagcggcag
     3661 ccgcctggcc gcccagcagc aaatggaggg cctgcttgcc ccccatcgtc ccaaggagcc
     3721 tgcctggttt ctggccactg taggagtgag ccccgaccac cagggcaagg gcttgggcag
     3781 cgccgtcgtg ttgcccggcg tagaggccgc cgaacgcgcc ggtgtgcccg cctttctcga
     3841 aacaagcgca ccaagaaacc ttccattcta cgagcgcctg ggcttcaccg tgaccgccga
     3901 tgtcgaggtg cccgagggac ctaggacctg gtgtatgaca cgaaaacctg gcgcctaatg
     3961 atctagaacc ggtcatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     4021 ttttttgtgt gttcgaacta gatgctgtcg accgatgccc ttgagagcct tcaacccagt
     4081 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     4141 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
     4201 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4261 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4321 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4381 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4441 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4501 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4561 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4621 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4681 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4741 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4801 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4861 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4981 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5101 aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat
     5161 cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc
     5221 cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat
     5281 gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag
     5341 ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg
     5401 tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc
     5461 tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca
     5521 gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg
     5581 gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc
     5641 actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta
     5701 ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc
     5761 tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg
     5821 ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc
     5881 cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc
     5941 aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat
     6001 gctcatactc gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc
     6061 tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat
     6121 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     6181 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     6241 gtgcaggtgc cagaacattt ctct
//
