LOCUS       sequence-224168-        3223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with Rab31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-Rab31
FEATURES             Location/Qualifiers
     source          1..3223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(709..804)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(841..860)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(842..860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(865..881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(865..881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(983..1002)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             994..1803
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1061..1080)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1950..2538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2439..2458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2692..2709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2868..2895)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2987..3073)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3122..3144
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3136..3153
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3137..3153
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcca taagggagct aaaagtttgt ctgctcgggg acactggtgt ggggaaatcg
      181 agtatcgtgt gtcggtttgt tcaagatcac tttgaccaca acattagtcc gacaataggt
      241 gcatcatttt taactaagac cgtcccctct ggaaatgaac ttcacaaatt tctgatctgg
      301 gacactgcag gacaagagcg gtttcattcc ctggctccca tgtattacag aggctcggca
      361 gcggctgtca ttgtttatga catcacaaag cttgactcgt tccagacgtt aaagaagtgg
      421 gtgaaagagc tgaaggagca cgggccggag gatatagtgg tggccatcgc tggaaataag
      481 aacgatctgg gtgacatcag ggaagttcca actaaggagg cgaaagaatt tgcagagtcg
      541 attgcagcca tatttatgga gaccagtgcc agaaatgctg taaacataga ggagctgttc
      601 caaaaaatca gtcgacagat ccctccactg gagaacactg atgcagacag ccacgactcc
      661 ttcaagctga cccgacagcc tcctccagcc accaaacgct gctgttagca actttattat
      721 acaaagttgg cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta
      781 tcagtcaaaa taaaatcatt atttggagct ccatggtagc gttaacgcgg ccgcgatatc
      841 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
      901 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
      961 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1021 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1081 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1141 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1201 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1261 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1321 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1381 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1441 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1501 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     1561 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1621 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1681 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1741 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     1801 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     1861 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     1921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2761 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     2821 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     2881 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     2941 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3001 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3061 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3121 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccctg cagctctaga
     3181 gctcgaattc tacaggtcac taataccatc taagtagttg gtt
//
