LOCUS       sequence-224181-        3256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab18a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab18a
FEATURES             Location/Qualifiers
     source          1..3256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(742..837)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(874..893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(875..893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(898..914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(898..914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1016..1035)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1027..1836
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1094..1113)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1983..2571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2472..2491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2725..2742
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2901..2928)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3020..3106)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3155..3177
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3169..3186
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3170..3186
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgaatg atgatatatt gaccactctg aaaatactga tcattggcga aagtggagta
      181 ggcaaatcca gtcttctttt gagatttact gatgatacat ttgatgcaga aattggtgca
      241 acaataggtg tggatttcaa ggtgaaaacg ctggctgtag atggaaaccg agcaaaacta
      301 gcaatctggg atacagcagg tcaagaacgt tttcggactt taacacccag ttactacaga
      361 ggagctcagg gtgtcattct ggtgtatgat gtctctaggc gagagacatt tgctaagctg
      421 gataactggt tgagtgagct ggagacgtac tgtacaagaa atgatcttgt caagatgcta
      481 gtgggaaaca aaatcgataa ggatgatcgt gagattgagc gagaggaggg cctgaagttc
      541 gcaagaaaac actccatgct tttcatagag tccagtgcta aaacaaggga tggtgtccag
      601 tgtgcctttg aggagctggt ggagaaaatc ctgcagactc caggcttgtg ggagagtatg
      661 cataagtcaa gaggagttgc cctgtctgaa ctctctgaaa gtagtcaagg aggctgcggg
      721 gcgtactgct cacttctcta acaactttat tatacaaagt tggcattata aaaaagcatt
      781 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgga
      841 gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta ttacatggtc
      901 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
      961 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1021 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1081 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1141 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1201 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1261 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1321 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1381 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1441 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1501 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     1561 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     1621 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     1681 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     1741 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     1801 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     1861 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     1921 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2101 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2341 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2641 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2701 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2761 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2821 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     2881 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     2941 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3001 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3061 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3121 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3181 gccagtctta agctcgggcc ctgcagctct agagctcgaa ttctacaggt cactaatacc
     3241 atctaagtag ttggtt
//
