LOCUS       sequence-224183-        3266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab18b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab18b
FEATURES             Location/Qualifiers
     source          1..3266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(752..847)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(884..903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(885..903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(908..924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(908..924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1026..1045)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1037..1846
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1104..1123)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1993..2581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2482..2501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2735..2752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2911..2938)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3030..3116)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3165..3187
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3179..3196
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3180..3196
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggacg acgacgtgtt gacgaccctg aagattttaa taatcggaga gagcggagtc
      181 ggtaaatcga gtttgctcct gcgtttcaca gatgacacct tcgacccaga attagcagca
      241 accatcggtg tggacttcaa agtcaaaact attgcgatcg atggcaaccg agcaaaactg
      301 gctatatggg ataccgcagg tcaggagagg ttcagaactc taacaccaag ctactacaga
      361 ggggcccagg gtgtcatttt ggtgtatgat gttacaaagc gggacacgtt cacaaagctt
      421 gagaactggc tcaatgagct ggagacatac tgtacacgca acgacctggt taaaatgctg
      481 gtggggaaca aaatcgacaa ggacaaccgt gaagtggacc ggaacgaagg cttgaagttt
      541 gcacggaaac actctatgct ctttatcgag gccagcgcga agacgcggga cggagttcag
      601 tgtgcctttg aggagctggt ggagaagatc ctgcagacgc cggggctctg ggaaagcagc
      661 atccagaacc atggtgtcca gctgtctgat aatgaacccc agcgtcaagg agcctgcggc
      721 ggatactgct cactggttta aggatccacc gcaactttat tatacaaagt tggcattata
      781 aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      841 attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta
      901 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      961 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1021 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1081 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1141 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1201 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1261 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1321 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1381 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1441 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1501 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1561 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1621 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1681 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1741 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1801 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     1861 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     1921 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     2881 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     2941 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     3001 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     3061 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     3121 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
     3181 taaaacgacg gccagtctta agctcgggcc ctgcagctct agagctcgaa ttctacaggt
     3241 cactaatacc atctaagtag ttggtt
//
