LOCUS       sequence-22420-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  TITLE     Id1 plasmids
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3 mId1
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(517..533)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(517..533)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(568..586)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(569..586)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(606..623)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttgatat
       61 cgaattctat gaaggtcgcc agtggcagtg ccgcagccgc tgcaggccct agctgttcgc
      121 tgaaggcggg caggacagcg ggcgaggtgg tacttggtct gtcggagcaa agcgtggcca
      181 tctcgcgctg cgctgggacg cgcctgcccg ccttgctgga cgagcagcag gtgaacgtcc
      241 tgctctacga catgaacggc tgctactcac gcctcaagga gctggtgccc accctgcccc
      301 agaaccgcaa agtgagcaag gtggagatcc tgcagcatgt aatcgactac atcagggacc
      361 tgcagctgga gctgaactcg gagtctgaag tcgggaccac cggaggccgg ggactgcctg
      421 tccgcgcccc gctcagcacc ctgaacggcg agatcagtgc cttggcggcc gaggcggcat
      481 gtgttccagc cgacgatcgc atcttgtgtc gctgaggatc cactagttct agagcggccg
      541 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
      601 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
      661 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc ta
//
