LOCUS       sequence-224219-        8469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for measuring protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Gao XJ, Chong LS, Kim MS, Elowitz MB
  TITLE     Programmable protein circuits in living cells.
  JOURNAL   Science. 2018 Sep 21;361(6408):1252-1258. doi: 
            10.1126/science.aat5062.
  PUBMED    30237357
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-09-21- 21"; volume: "361"; 
            issue: "6408"; pages: "1252-1258"
COMMENT     Sequence Label: PB-CMV-FKBP-Zte-Cit-hc-DHFR
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1409..1788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1789..1992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1943..1963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2037..2056
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2037..2055
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2092..2101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2542..2562
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             join(2575..2577,2578..3288)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2617..2638)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2878..2897)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3225..3246
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3849..3866)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3855..4062
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4095..4115)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4100..4429
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4280..4415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4342..4361
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4477..4524
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4543..4917
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(4977..4997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5039..5160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5076..5095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5130..5149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5923..5957
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6448..6464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6448..6464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6461..6483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6472..6488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6496..6502,6503..6520,6521..6526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6541..6562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6679..6696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6850..7438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6930..6949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7609..8400,8401..8469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8232..8251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtaggcgttt tgcgctgctt
     1381 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     1441 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1501 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1561 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1621 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1681 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1741 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1801 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1861 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1921 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1981 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     2041 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt cgccaccatg
     2101 ggaggagttc aggtggagac catatctccg ggggatggca ggacatttcc gaaaaggggc
     2161 cagacgtgtg ttgttcatta tacagggatg ttggaggacg gaaaaaaggt agactcttct
     2221 cgggatagaa ataagccctt taagttcatg ctgggtaagc aggaagttat tcgaggatgg
     2281 gaagaaggag tagcccagat gtcagttgga cagcgggcaa aattgactat aagccctgat
     2341 tatgcctacg gtgcaacggg tcacccaggt attataccgc cgcatgcgac actcgttttt
     2401 gacgtggaac ttttgaaacc tgaaggaggc ggatccgagc aactcgaaaa gaagctgcaa
     2461 gcgttggaga agaagctcgc acaacttgaa tggaaaaatc aggcactgga aaagaaactg
     2521 gctcaaggtg gctccggcag cgagaatctt tatttccaat ccggctcagg ctccgtgagc
     2581 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2641 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2701 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2761 accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     2821 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2881 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2941 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3001 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3061 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3121 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3181 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3241 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg tgggtccggt
     3301 ctcgacgaga tggaggagtg tagtcagcac cttgggagcg gatcgatatc actgatcgct
     3361 gcactcgcgg ttgattatgt aataggcatg gaaaacgcga tgccctggaa cctgccagca
     3421 gacctcgcat ggtttaagcg gaacactctg aataagccag tcattatggg ccgacatacg
     3481 tgggaaagca ttggacgccc actgccagga aggaagaata taatcctgtc atcacagccg
     3541 tccacggacg atagagtcac ttgggtaaaa tccgttgacg aagccatagc tgcctgtggt
     3601 gatgtgcctg agataatggt aataggagga gggcgggtaa tcgaacaatt tctgcctaaa
     3661 gcccagaagc tgtaccttac ccatattgac gcggaagtag agggcgacac acatttccca
     3721 gattatgagc cagatgattg ggagagcgtt ttctccgaat ttcatgatgc agacgcccag
     3781 aatagccaca gctactgctt tgaaatattg gagcgccgat gaagctagcg aattcgaatt
     3841 taaatcggcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3901 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3961 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4021 caagggggag gattgggaag acaatagcag gcatgctggg gacgcggccg caatttaacg
     4081 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4201 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4261 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4321 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4381 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4441 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     4501 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     4561 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     4621 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     4681 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     4741 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     4801 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     4861 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgagtc
     4921 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4981 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc gttaactaaa
     5041 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5101 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5161 tcatgtctgg aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc
     5221 cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt
     5281 cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag
     5341 tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag
     5401 ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     5461 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     5521 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     5581 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     5641 ctgtccctga tatctataac aagaaaatat atatataata agttatcacg taagtagaac
     5701 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc
     5761 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg
     5821 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg
     5881 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc
     5941 agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc
     6001 ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct
     6061 tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg
     6121 acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct
     6181 ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga
     6241 cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc
     6301 ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca
     6361 gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac
     6421 ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8461 aatactcat
//
