LOCUS       sequence-224222-       14478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU31.1-2.2
FEATURES             Location/Qualifiers
     source          1..14478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8987..9007)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8987..9005)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9344..9932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9424..9443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10103..10894,10895..10963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10726..10745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10964..11068)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(11995..12042)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12415..12433
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12705..13112
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13113..14207
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14454..14470)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14454..14470)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggctt
      541 cagtgccaaa tggtaacggc cacccggcca gcatatccga caaacaggaa ccgatggcca
      601 taatcggaat ggcaacccgt ttccctcaag aagctaccaa cacagagaaa ttatgggggt
      661 ttttgttgaa agggaggtca gctcatactg cgtttccaaa agacagaatc aatccggatg
      721 gacactatca ccccgacccc gagcatggag gcacatttgc cgtcaagggg ggccattttc
      781 tttcagaaga ccccgcatat tttgatgccc cgttctttgg catcacaaag acggaggtcc
      841 tctctctgga tcctcaacag agagtgataa tggagaatgt ataccaagcg cttgagaatg
      901 ctggtcttcc aattgccaaa gtgaccggaa ccaacacgtc agtctacgtt tcgggattca
      961 accacgatca ccttgcaaca ctcaattccg atcctgaaac gaccctgaag tacaagccca
     1021 cgggcacgac gaattctctt ttgagcaacc gcgtttcgtg gtttttcgac ttcaaggcac
     1081 caagtttaac cttagataca gcatgttcca gcagtatggt agctctgcac ttggcttgcc
     1141 aaagtttaag atgcggagct gatgctgagt ttagtccgtg catggggcat cacggattcc
     1201 tctcgcagga aggacgctgc ttcagctttg atcatcgtgc ggacggctat gcccgcggcg
     1261 aaggggtagg tactgttata gtgaaacggc tctcggatgc gctcagggac ggaaacacta
     1321 ttcgagcggt aatacgaggg actggtgtca accaagacgg acgaacagcc gggatttctc
     1381 ttcccagcag tgctgctcag gaaagactca tgagaaccgt ctatgcaagt gcaggactgg
     1441 attttaatga cactatgatg gtggaggcac atgggacggg cacagccgcc ggtgatccta
     1501 ttgaagctag tgctattgct cggacattcg cgtcaaggag caaagatatt ccactgtttg
     1561 ttggagccat caaatctgga attggacatt tggaaggcgg tgcgggcatt gctggaataa
     1621 taaagagcgt cctggtccta gagagtggga taatcccccc gaatgtgaat ttcgagaagg
     1681 ccaaccctaa aatcccaatc gaagcttgga atcttcaatt tcctttagta aacacgccgt
     1741 ggccgactcc tggcctgaga cggatatctg taaattcgtt cggagtcggc ggcacaaatg
     1801 gacacaccgt gctcgatgac gcatataact atcttacttc ccgaaatcta acgggtgctc
     1861 ataataccaa ccatcatact ccaacggccg aagaaataag agttaccgtg gcggagattg
     1921 atggctctat agccctagaa accgaaatgg acgccccacc gaacggccac gccaatggaa
     1981 cgtctaacgg ggcaactacc aacggcaatc atgataccac agaaataact aacggtaatg
     2041 gtgttcaaaa cggcaacgga gtcaataata atggccactt tgcccctcat gtccctcgcc
     2101 tcttcgtctt ctcttctttc gatgagaaag gggccaagcg aaacgcaacc acatacgcca
     2161 agtacctgaa gaatatccag ccccccaatg aagatgacta catgaaagat ctggcctaca
     2221 ccttgtctga aaaacgctcg gtattcccat ggagaagcta tgcagtgtct aactccttgt
     2281 cggaactgat tgagaacctg tcaagtgacc agggaatgcc aagcgctgtg cgggtaggga
     2341 caccacctag gattggcttt gttttcaccg gacaaggcgc gcaatggtac gctatgggta
     2401 gagagctgtt gatatatacc atttttagga agagcctcga ggacgctgaa tcatatatga
     2461 agtcaatcgg cgcggaatgg tcgctgctag aagagctttc ccggagcaaa gaggagacaa
     2521 gattaaatga gccgcatctg gcacatccgt cgtgcacagc cgtacagatt gctctcgttg
     2581 aactacttac cagttggaac atccgaccta cgcgagtggt cggccactcc tctggagaga
     2641 ttgccgccgc ctactgtgca ggtaagctta gtaaggaagc cgcatggaag gttgcatatt
     2701 tccgcggcta tgtctcagcg aagcaacgtg gagcaaaagg ggctatgatt gccgttgcgc
     2761 tgtccgaaac tgatcttcag ccgtatttga aaaccatcaa cgagctgcat gagggggaga
     2821 tgataatagc ttgttacaat gctcccaaga atcacaccat ctccggggat gaacacaaga
     2881 tcgatgccct caaaactctc ttggatgata aaaaggtctt tgctagaaag cttaatgtgc
     2941 tcaacgctta ccactccggg catatgaagg cccttgcaga cgagtaccta gaacgcatga
     3001 gcgatatctc ttttggcgat cgcctgtctg tggcagatat tcacttgttc tctagtgtca
     3061 atggccacaa agtcgagaaa gacactctgg atgcgcaata ctgggtcgac aatatggtgt
     3121 ctcctgtccg atttaccgat gcgctgttgg caatgagctt tactcggcta tcgaagggac
     3181 aggcgtcgtt gcgtttgaat gccggcactg agaacatctt cgttgatgct attgtcgaga
     3241 tcggacctca tgctgcattg cagagcgcta tcaaagatac actggcaacc aaagtcagca
     3301 gctcctcgtt ctcatacttg tcagtcctta acagaacgac tcctggcgct cagattatac
     3361 tcaatagtgc cggagttttg tgctctcgcg gttcgcccgt aaatatccag gcggtgaatg
     3421 attcagccaa cccaggcgcc accgctccac ggttgatcgt ccaactccca ccctacagct
     3481 tcaatcactc tgataaagtc tggtatgaga gcagactaag caagaactac cgcctccgca
     3541 aatatccgcg gcatgacctc ttcggggctc ccgtctctga ctggaacagt gagagacccc
     3601 actggcgcca tattatcaga ctctacgaac agccgtggct cagagatcat atggttactg
     3661 atgcctatgt ttatcctgga gtcgggtata ttattgctgt tatcgaagcc tctaggcaga
     3721 tagcagatcc ggagatgaca atcacggaat tccgcctccg tgacgtctct ctgaaacgag
     3781 ctctcattgt ccctgacaac aaggaaggta ttgagattaa cctttcgttt gctagaatgg
     3841 acgaatccag cttgtggggt tcgtctgtgt ggaagaaatt cgtgatctct agctggaatc
     3901 ccactggaaa cgattggata gagcattgta ctggttatgt cagcgttgat tatgagactt
     3961 ctcccaatct tgtcgatgct ggacgggaat cagtggccga ggccctcaag tggaaagata
     4021 cactggaggg tgtcaaacaa cgatgcgtta tacctttcga tattgacacc acctacgaca
     4081 acctcattac tagtggattg gcctttggcc ctctcttcaa aaacctatct gacgtacgag
     4141 gaacggccga tgcacgtgga gaagtttttg gcttggtcac tgtgccagat attgccgcag
     4201 caatgccgaa aggcttcact caccctcacc tcatccatcc agcgacaatg gacagcatga
     4261 tgcacttctt catcgcctca gtcatggacc acacaggtaa gaacacccta cggacggcaa
     4321 tggtccctac gttcataaaa gacgtgtgga tctctgccca ggtggacaat aacccttctc
     4381 ataccttccg tggacacgga aagtctactt tcctcgcata tgacaaatac cagagcgatg
     4441 tcatggtatg ggatggcgcc acagacgagg ctcgtatctc aatcaaagga attcgcgccg
     4501 ctcctctcga atcgattgat agcggatcag gcgagtcccg aaaactctgt cataatatcg
     4561 aatgggggct cgatgccgat ctcctcagcc ccgcatcatt ccccgccgtt gtccacgcca
     4621 ctcatgaaga taatcttgat tacaagtact ggatcaacag gttccagctt gcaaccttgt
     4681 tgcgggtcac tgacgctctc tctgctctgg gagacttcaa taccgatgta ctagaaggcc
     4741 atttcctaaa gtactggaac tggttgaagc aactagaaga gtggctgcac gctgatcaga
     4801 tcagcggtat gaaattgagc gactggacag agtacaacaa tgacccgtcg ctcaaggcac
     4861 agttatttga agaagtcgct gcccacaatg cggatggtgc gctcgccatg cgaatgggat
     4921 ctaacattac ggcagttctt cgcaaagaag tcgacccgtt gcatctcatg tttggtcaag
     4981 acgatcttct taaccacgtg tatgagcaag tcgtcaagtt aggcgatctg ccaaatcttc
     5041 aaaaggcata tctcgagatc gtagcgtata actccactaa ccttcgtatt ttggaagtcg
     5101 gtgctgggac tggaagctca acagctgcaa tgcttgacgg gctctcgcag agtactgacg
     5161 gcgggaagaa tatgacttca agaatcgaaa cgtatacctt tacagatgtc tcgtctggat
     5221 tctttgagaa agctcaggag aaattcaagg catacaaaaa tattctagag tacagaatat
     5281 taaacgtcga gaaagatgtc tctagtcaag gattcgatgt taaaagttac gacttcatcg
     5341 tggctggaaa tgtcattcat gctactgccg acatcaagaa atctctcacg aatttgcaag
     5401 agcttttgaa gcccggcggg aaactgattc tgcatgaggg cattagacaa gattatctat
     5461 ggtcgggcat ctcttttggc cagttgccgg gctggtggct aggtgttgaa cctatccgtc
     5521 agtggaaccc ttggattacg gcggttcagt ggaatgtaat cctgaaggaa gcgggttttt
     5581 caggggttga tcttgacctg ccagatcgaa aggacccgaa ccttcatacg caaagtctct
     5641 tgatcgcaac agccgttgag ccctctggtg caatgcccca gaccgtcatc gtgacaacca
     5701 ccccttcttg cgattccgaa ctgatcacag ctctaaaatc gcatcttgag ttcaccctca
     5761 aaataccaaa tgtctgcacc gtgcactacc tcgatcttgg taacaccgac ctcagcaaag
     5821 cagtctgcat ctccgtcatg gaactcgagc gctccgtcct ctcaaccctc gctatgacag
     5881 agtataacaa catccgccac ctccttgtca catgtgtcgg catggtttgg attactggcg
     5941 acacactcac caacccatcc ttcaacatga tcaccggact gacgcgcact gtgcgctggg
     6001 aacgcgacat tgacgaggta aatctcatca ctctctcagt gtgcgaacct aggcccgctg
     6061 ttagtgacct tgtatcatcc attgggagac tatacagcca gcaatttatc gcaacgttac
     6121 cggctgacaa gatcaacagc gagtacatgc tgcgccacgc atcattctta tcagcgcggc
     6181 tcatggactc agacactggt aatgattatc tgtcttcaaa aactgtgcgg cccacacccg
     6241 tgctccaaga gcttagcaaa gccggaagac cgctgaagct ggctactgca tcgcctggac
     6301 agctagacaa gctggagtgg gtgacagatt ctatttatgc tgagccgctt agtgagacac
     6361 aggtggagat tgagatcaag gctgtggggt tgaattttag ggatttgatg attgcgatgg
     6421 gtgagcatat ggcgtatagt cttggaaacg aagcagcggg tatcatttcc cgcgtcggct
     6481 tgggcgttac agcctttaag ccgggtgacc gtgtcgttta catgtgtggc ctagagagca
     6541 caggttgctt ccagacatat ggccgcgtcg atcagaacgt ggtggttcta atcccagaca
     6601 gtctcagcta cgagattgcg tgcagtttgc cttgcgtata ctccactgtc atttatgggt
     6661 taatggatgc ggcaagattg ctaaagggag aaaaaatact gatccatgcg gctgctggag
     6721 gtgtcgggca ggcggctatt aacctctcga aaatgattgg agcggagatc ttcgcaacgg
     6781 tgtcgacgcc ggaaaaaaga gcgctccttg tgaaagagta tggcattaat gaagagcaca
     6841 tcttctcgag ccgagatctc acgtttgtcc aaggcataat gcgagtcacc aatggcaagg
     6901 gcgtagacgt agttttgaac tcgctttctg gagaggctct tcgtcgctct tgggatctta
     6961 ttgctccctt tggccgcttc gtcgagattg gtaagaaaga cgcccaagct aatggcaaga
     7021 ttgaactcca cccgttcttg cgtaatgtca ccatgacaag tgtcgaacta cccacaatga
     7081 tgcgccatcg acctcatctt attcaacgac tgacggccga caccatcaaa ctatatgacg
     7141 aaggtaagat taaggaggct ataccaacga atgttatggg ttatgataga attgaggaag
     7201 gattgaggat gttgcagtcg ggtaagggca tggggaagat ggtattccga ccgtcgccta
     7261 gtgatatcgt gcccattgtc cccgaacaaa cgactcctta ccgagttgaa gcggatgcaa
     7321 cttacgtgct tgctggtgga ctcggtggca tcggtcgtag tatggcgaag tggttggtag
     7381 agcgtggagc gaagagtctg gtgtttcttt cgcggtctgg gagggtcaca gatgctgtgg
     7441 gcaagatgat gttggatttg aagacggcgg gatgcaatgt taaagtcttc acttgtgatg
     7501 tgactgacaa gatgaggctt gcggaggtga tggatgagtg caaggttacc ctgccaccga
     7561 tcaagggttg cattcaaggg tccatggttt tgaaggacgt tatgtttgag aatatgtcgt
     7621 acgatacatt cgaggcagct gtgaagccca aagtccaagg ctcctggaac cttcacgagc
     7681 tcctacctgc aaacatagat ttctttgtcc tcctctcctc ggctactgga attctaggaa
     7741 accgcagcca agccaactac gccgccggca acacctacca agatgcccta gcaaggcacc
     7801 gcatctccaa aggtcttcct gcagctgtca tcgatcttgg taccgtcatg tccgtcgggt
     7861 atgttgctga aaataaagaa agggccagta tggccaagca tctcggaacg gtgctcgaag
     7921 tactccgcga agacgagata catactctca tcgagtacct catggatccg cgctcgaaac
     7981 tgtccgagtt atcatgccaa ctcgtctctg gcttgactac cgcagccatc taccgcgaac
     8041 gtggaatccc aacaccgacg tatctgagct accccctctt cacccatctc cgtacggcat
     8101 caagttcgcg aagtgaaggc tctgaagaag acccctcctt tttggtacag gcccagctgc
     8161 gtgccgcgac gtctctcgag gaggctactg cagtcgctgc caatggcgtg cgggcgaagc
     8221 tgtcgtctct acttgccgtt ccacaggaca atatcgaccc gacaaagtcc atcagctcca
     8281 acggtgtgga cagtttggtc gctatggagt tccggaccta tctaacgaag gatttgggcg
     8341 ccgatgtgcc gttgttggat atcatgggca ctagcagtat tatggtgcta agtcagaaga
     8401 ttgcgagtgt gagtaagttg gtgttgattt cggatgagaa ggagacttga ggagattgat
     8461 aagacttttc tagttgcata tcttttatat ttaaatctta tctattagtt aattttttgt
     8521 aatttatcct tatatatagt ctggttattc taaaatatca tttcagtatc taaaaattcc
     8581 cctctttttt cagttatatc ttaacaggcg acagtccaaa tgttgattta tcccagtccg
     8641 attcatcagg gttgtgaagc attttgtcaa tggtcgaaat cacatcagta atagtgcctc
     8701 ttacttgcct catagaattt ctttctctta acgtcaccgt ttggtctttt atagtttcga
     8761 aatctatggt gataccaaat ggtgttccca attcatcgtt acgggcgtat tttttaccaa
     8821 ttgaagtatt ggaatcgtca attttaaagt atatctctct tttacgtaaa gcctgcgaga
     8881 tcctcttaag tatagcgggg aagccatcgt tattcgatat tgtcgtaaca aatactttga
     8941 tcggcgctat gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     9001 taattgcgcg cttggcgaga ctcaaacaag atatattttg cccgccttac agacgaaact
     9061 agttggaggt tatggagcat actatcacgt gggcggccac tggtgagtta ctacacccca
     9121 ggggcaacgt tgatgctcct aaaaaactct ggctggacgc aagccgtaac acccgtgtca
     9181 cttcataatc gtttgcaatt cagggcttga tctacacatt accctgttat ccctattaat
     9241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9781 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa
    11101 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
    11161 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
    11221 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctattttacc
    11281 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
    11341 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
    11401 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
    11461 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
    11521 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
    11581 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
    11641 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
    11701 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
    11761 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
    11821 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
    11881 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
    11941 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
    12001 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
    12061 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
    12121 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
    12181 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
    12241 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
    12301 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
    12361 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
    12421 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctcggcgcg
    12481 ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc
    12541 ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt
    12601 gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg
    12661 cgggtattga accaggctta tgcccaagat cgtagcaagc atttaactgt gggaatactc
    12721 aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat
    12781 aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg
    12841 ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag
    12901 gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa
    12961 tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa
    13021 taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat
    13081 atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt
    13141 tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta
    13201 tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg
    13261 ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg
    13321 atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg
    13381 aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta
    13441 actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta
    13501 ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag
    13561 acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa
    13621 tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct
    13681 tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca
    13741 tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga
    13801 tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg
    13861 atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt
    13921 ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca tgccacggtt
    13981 ctgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg tctgctgcaa
    14041 tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa gatgcagtta
    14101 aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccaccg
    14161 aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt
    14221 ttttatgata tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat
    14281 tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta catacattta
    14341 tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc
    14401 tcaacgaaat gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac
    14461 tagttctaga ttaattaa
//
