LOCUS       sequence-22423-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Aakalu G, Smith WB, Nguyen N, Jiang C, Schuman EM
  TITLE     Dynamic visualization of local protein synthesis in hippocampal 
            neurons.
  JOURNAL   Neuron. 2001 May . 30(2):489-502.
  PUBMED    11395009
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2001 May . 30(2):489-502."
COMMENT     Sequence Label: pcDNA3.1 5'-myr-dEGFP-3'
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(328..349)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(589..608)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctctggcta ctagagaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag cgtttaaact taagcttcag aagccccaag ctcgtcaatc
      121 aagctggttc tccatttgca ctcaggagca cgggcaggcg agtggcccct agttctggga
      181 gcagagtatc agcatcccag tcctagtccc gagcctaaag cctcgcctgc ctgcccagtg
      241 ccaggatcca tgggcacggt gctgtcccta tctcccagct ctagagtgag caagggcgag
      301 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      361 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      421 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      481 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      541 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      601 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      661 aagggcatcg acttcaa
//
