LOCUS       sequence-224232-       18242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18242)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 18242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU20.1-2.2
FEATURES             Location/Qualifiers
     source          1..18242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      887..1186
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3813..4033
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4034..4837
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      11354..11541
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(11409..11433)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(13862..13882)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13862..13880)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(14219..14807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14299..14318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14978..15769,15770..15838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15601..15620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15839..15943)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15970..17312
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(16933..16980)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17353..17371
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17713..17729)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17713..17729)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcttcttcta ctattctctt ttgggtgctc catcttggaa cctaccttgt atcatgtata
       61 cactttatat ctattcacct tgactgacta caagaccatt tttattccga ttacggtttt
      121 cgcctgctct acagctccca tacattcgat ctccaatctt ttccatggaa gcacatggat
      181 atggatgcac ctactgatgt gtaatgtctc gaatcaactg agaggggagc aggaagacgc
      241 cgtgaatcga ccatggagac cactgccgtc gacccggatg accaagaggc aagccacgac
      301 gctccggtgg gctatggtgg tgttatgcct gctctactct tccgcctatg gtcaccgatt
      361 agcatacgtt actgtcggcc ttttcatcac aacatacgta tacgacgatc tagggttctc
      421 tcagagtccg ctcggaaaaa gcttttgtaa cgttggtggt tatgcgactt tcgagatcgg
      481 cgccacaaag atcatgggtg cagaacacga gctagacact acctcacttg cgtccgtctt
      541 tcttagcggc atggtgatat ttactactat ccaagtacag gactatcccg acatcgaagg
      601 cgacacagcg tctggtcggg tcacctttcc aatttatgca cccgagttct cgcgattatt
      661 cactctgtca tcgatcatta tctggtcctt cgccttgtca tggttctggg atatcggcat
      721 cctgagtcag tgctttctcg caggattggg agtatacgtc ggtgctcgtt actatctcca
      781 tcggacacca atcgcggata aaaaatcata caagctcttc aacgtttggc tggtgctgat
      841 acatcttctt ccactccacg cccgcatccg tgtgtttctc tattaggcgg atctcttatg
      901 tctttacgat ttatagtttt cattatcaag tatgcctata ttagtatata gcatctttag
      961 atgacagtgt tcgaagtttc acgaataaaa gataatattc tactttttgc tcccaccgcg
     1021 tttgctagca cgagtgaaca ccatccctcg cctgtgagtt gtacccattc ctctaaactg
     1081 tagacatggt agcttcagca gtgttcgtta tgtacggcat cctccaacaa acagtcggtt
     1141 atagtttgtc ctgctcctct gaatcgtctc cctcgatatt tctcattttc cttcgcatgc
     1201 cagcattgaa atgatcgaag ttcaatgatg aaacggtaat tcttctgtca tttactcatc
     1261 tcatctcatc aagttatata attctatacg gatgtaattt ttcacttttc gtcttgacgt
     1321 ccaccctata atttcaatta ttgaaccctc acgatccagt tctccagtga cacagccttt
     1381 atctggtcaa acctttcttt ctaatcacct atgctgatgc ttaattaagg gatttttgtc
     1441 tccatcaacg gcatgcgccc aaaaatgacg ttttttttaa cccatagaca cgaaactacc
     1501 cattttccac cggcctgacc taccaccgga acaacggcca tctccaactt gcaagttggg
     1561 gaaattaaga gcatcgcagg tttaatggaa gaaaaaaaaa aggtacagca cagcgcaaat
     1621 ggagttagtt cccttatgtc acacactcac acacagtcgg tcagatcaag catactgggt
     1681 gcgtataaat agagtggcca ttgccaccct gtttatctca aaatctgtct tgttagtggt
     1741 cttctccctt tttcaggtta caattctctt gtttctactt agtatataag tatatcaagc
     1801 tatattaagc atactatcaa ctgtcaactc tatcctcaaa atacaataca aaatgaagcg
     1861 cttgccttgc attgcccttt caccctacgc ccatcttcct acgctgcgca tcatgaacta
     1921 tatgctatcc tttctcgttc tacttgtatc acttttgatg ctcagaatca tactgaagag
     1981 gaacacagac aactctggtg cccatcatta tcctcctggt ccgcccgcaa tacccgtcct
     2041 tggtaatatc atgactattc cgaacacggg accatggaaa ttattctcaa agcttcatcg
     2101 tgactacgga gaacctatct tcttttgggg cttagggaac agcatccttg ttctcagcac
     2161 aatgacttcc ataacagatc tcctcgacaa gcgaggggag aactattctc atcggccggt
     2221 attgactgtt ttaggagaac tgatgggatt ggatcgtagc atgccgatgt taccgtacgg
     2281 gcgtgagtgg cgagagcaga gaaagctcgc ccacgttgcc ttgaacccat ctgccgtgag
     2341 aaagtatcac atcgtgcagg agaacctagc ttctgtattg gcgcttggta ttctcacaaa
     2401 ccccgaagga ttctttgata atgtacgact ttacgcggag cgcataatat tctctgtggt
     2461 atacggtgtg tacatcgatg gttccaataa tgagtatgtc actcaggcag aagaaaccat
     2521 gaaaattgtg ggagacgcaa tggtaccagg agcgttcatc tgtgacttgt tgccttggat
     2581 gaagcatctt ccttcgtggt tgccatttcg aaagcaagca cattatggga gaagtatgat
     2641 tgaagccctt gtgaccaaac ccttcgaaca cgttcaacga gagatgatga cgaatatcgc
     2701 tgcaccatct ctcactcgag aactattgac tttgcaacgt aacgatttcg cgtcttttga
     2761 gagctccgtt aagtgggcga ctggatcttt atatggtggt aggcataaca tttgggtctt
     2821 ttcaaagaat ttttgtctcg cactgacggg aactggactg tcagcgggcg gcgaaacgac
     2881 ttacgccacc atccttactt tcatgatggc aatggcaatg aatcccgaaa ctcaacgtaa
     2941 agcccaaaag gaggtcgatg aggttatagg gatacataga cttcccgtcg tctcggacaa
     3001 gacacagctg aaatacgtag cagctgtcat caacgagaca atgagatggc atcctgtgtt
     3061 accgctgggc atcgctcgcc gtacggacca tgacgatgtg tacatgggat acttcatacc
     3121 aaagaatact gttgtttttc ctaatgcatg ggcgctcaca aggaggcaca gcgacacgta
     3181 cgatccagaa caattcatcc cggaacgttt tatcgaccct tccgaagcaa cggacgatcc
     3241 ttccacttat ttgtttggat ttggaagaag agtctgtccg ggtaaatctc tggcggagaa
     3301 ctcggtattc attgcaatcg cgactatcct atctgcccta gatatctcag aagacccgga
     3361 cagtagaata accccagcat ttacggaagc tttagtaagg tatgattctc cccgggagct
     3421 ctacaaatga acaaatcgct cttaaatata tacctaaaga acattaaagc tatattataa
     3481 gcaaagatac gtaaattttg cttatattat tatacacata tcatatttct atatttttaa
     3541 gatttggtta tataatgtac gtaatgcaaa ggaaataaat tttatacatt attgaacagc
     3601 gtccaagtaa ctacattatg tgcactaata gtttagcgtc gtgaagactt tattgtgtcg
     3661 cgaaaagtaa aaattttaaa aattagagca ccttgaactt gcgaaaaagg ttctcatcaa
     3721 ctgtttaaaa ggaggatatc aggtcctatt tctgacaaac aatatacaaa tttagtttca
     3781 aagatgaatc agtgcgcgaa ggacataact cattcaattc aattcatcat ttttttttta
     3841 ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa
     3901 ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga
     3961 agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa
     4021 acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc
     4081 tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt
     4141 ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg
     4201 tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc
     4261 gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga
     4321 cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc
     4381 agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc
     4441 ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa
     4501 gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga agagcgacaa
     4561 agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga
     4621 ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca
     4681 gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg
     4741 actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg
     4801 ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat aagtaaatgc
     4861 atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat taccctatgc
     4921 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggata ggaaaaaacc
     4981 gagcttcctt tcatccggcg cggctgtgtt ctacatatca ctgaagctcc gggtatttta
     5041 agttatacaa gggaaagatg ccggctagac tagcaagttt taggctgctt aacattatgg
     5101 ataggcggat aaagggccca aacaggattg taaagcttag acgcttctgg ttggacaatg
     5161 gtacgtttgt gtattaagta aggcttggct ggggatagca acattgggca gagtatagaa
     5221 gaccacaaaa aaaaggtata taagggcaga gaagtctttg taatgtgtgt aacttctctt
     5281 ccatgtgtaa tcagtatttc tacttacttc ttaaatatac agaagtaaga cagataacca
     5341 acagcctttc ccagatatac atatatatct ttatttcagc ttaaacaata attatatttg
     5401 tttaactcaa aaataaaaaa aaaaaaccaa actcacgcaa ctaattattc cataataaaa
     5461 taacaacatg gcgactgtca tgtattctct tctcgttacg aactgcaccg tagttcacgt
     5521 tgctgtgctc atttcaatcg tgcttgtata ccgcaggctt tttgtaggac ggagatcttc
     5581 tcttgatccg ttgccctatc cagatgaaga accggagtgg atatggggtc acgaaaaacg
     5641 cgtttatgac agccctccag ggcaggcgta cttacggtgg atgaagagtg ttgggcatac
     5701 ctaccgcatt cgtgctgccc ttggtgcccc tgacgttctt gttctgggcg atccgctcgc
     5761 catttcttat attctccaag agaaaattta cgactaccac catagcgctg tggtacgccc
     5821 tagggtggct cgcttcctgg gaaaaggctt aggttgggtc gagggagaat cagagcataa
     5881 acgaatgaga cgtcttgtca gccctgcatt ctcggcgcaa accgttaaag acatggaagg
     5941 cgaaatattt caagcgacgt ctacggctag tctcaatatc gaggactcgg acggtgaaat
     6001 tggcgtaaac atattgtcat ggacaaatcg aatgaccctg aattccattg gacgcgtcgc
     6061 attcttgtac gatttcgaag ccgggaagag ccgcgaggca cagcttatat tggacacaag
     6121 gcggaagtcg ctaaccggta ttgctcgata cgcctcgttc ttaactctga tgatgctcag
     6181 gcggttccct gctctcaata gccttcctgt acccgccctt caggcgcaaa gcctagtcac
     6241 gacaaccata aagtctgtcg tggccaagga gatcatagcg agaggttctt caacatctga
     6301 tctccgaact cgaggcaagg acctgctaag cagactcttg gtagcccatg atgagcagca
     6361 cctttcagtc gaggaactac atgagcacat atcgacattt ttagtttctg gacaggaaac
     6421 tacgagccaa acggtcgcgt tctccatctt cgagctttcc cgtcacccag agatccaaca
     6481 gcgtctcaga gaagagttgg acacatctta cccatcttct acaggcacgc cgcagtcaca
     6541 gctaccttat ctggatgccg tgcttaagga aacactaagg atgtatcctg cccttccgta
     6601 catggaacgt gtcgcaacta aatctgatag aattccgctt caaacgccca tcagtcacgg
     6661 tggagatcag gcgctacggg acattcgtgt tgaaaagggt cagaccgtca tcatcccaat
     6721 catatctctt caccgctctg atgctatctg gggcgatgga agtgtctttc gtcccgaacg
     6781 atggcttgaa gctttacctc atgaggcaca gcgtcttcca gggtggtcca acttacttgc
     6841 attcagtgat ggacccagag catgccctgg aatgcgaatg gcgatatttg ggtcaaagct
     6901 tatattaagc tctcttgtca agaaatatcg catagaaagt aataactcag aagtagttct
     6961 caggatttcg tcaagtttgc agccttgggt gattgatgat tcggaccaag gtccgcaagt
     7021 gcctgtaacg ctgcacatgc ggtaaagtgc ttttaactaa gaattattag tcttttctgc
     7081 ttattttttc atcatagttt agaacacttt atattaacga atagtttatg aatctattta
     7141 ggtttaaaaa ttgatacagt tttataagtt actttttcaa agactcgtgc tgtctattgc
     7201 ataatgcact ggaaggagaa aaaaaaggtg cacacgcgtg gctttttctt gaatttgcag
     7261 tttgaaaaat aactacatgg atgataagaa aacatggagt acagtcactt tgagaacctt
     7321 caatcagctg gtaacgtctt cgttaattgg atactcaaaa aagatggata gcatgaatca
     7381 caagatggaa ggaaatgcgg gccacgacca cagtgatatg catatgggag atggagatga
     7441 tacctccatt gggccgatga agttagtcga cggatagaag cggttgtccc ctttcccggc
     7501 gagccggcag tcgggccgag gttcggataa attttgtatt gtgttttgat tctgtcatga
     7561 gtattactta tgttctcttt aggtaacccc aggttaatca atcacagttt cataccggct
     7621 agtattcaaa ttatgacttt tcttctgcag tgtcagcctt acgacgatta tctatgagct
     7681 ttgaatatag tttgccgtga ttcgtatctt taattggata ataaaatgcg aaggatcgat
     7741 gacccttatt attatttttc tacactggct accgatttaa ctcatcttct tgaaagtata
     7801 taagtaacag taaaatatac cgtacttctg ctaatgttat ttgtccctta tttttctttt
     7861 cttgtcttat gctatagtac ctaagaataa cgactattgt tttgaactaa acaaagtagt
     7921 aaaagcacat aaaagaatta agaaaatgca tcttccattc gcctggataa tatcgcttat
     7981 tacggtcgtt ggagttacgt gctggatatg caaacggtgc tttgatatcc ggaatctctc
     8041 gcttccgcct gggccgccag acaacgtgca tgaaaatgaa cgcaagtgga tggcttactc
     8101 acggtggtcc aaagaatcgg gcattcaagg tcctctcgtc acactgtcca cggcaccaca
     8161 cccaatgatg gttgtcagcg gacgcacagt aatccgagat cttctgatag gaaagaaatc
     8221 gttttgttgc aggccacgca tacctatggc cgagctcatg ggtagacaaa acaatgttgg
     8281 cttcacgtac tacggaaagc gactcatcgc gatgcgaacc ctgcttcact ccgcccttag
     8341 cctgactgaa gttacttcca cgtggatgga actcatcaac taccaggcgg atgatctttg
     8401 tagagatttc attgtgtctc cggaacggtt ctacggtcat gttgaagcca atatacagaa
     8461 actcatcgga cgctttactt acggccacga gccaagtccc gagtatctac aaatggcggc
     8521 ccaggtgtcg cgacacactg ggaaagctgt ggaggcaggc cgatgggccg tgaacagtat
     8581 tccagctctt atgtgggtac cggcatggat gcccggtgct ggtttcaaaa gatacgccat
     8641 tgatgcaaga aagctgttca cgcgcctgct caatgaatct gttgcgctcg taaaacagag
     8701 aatagaccaa aatgaagcgg acacgtcctt tgttaaacaa tctctgacca acatctcgct
     8761 atatccatca tgtatcgatg agtcacttgt tatatcttcc gcggggagct tgtttagtgc
     8821 agggacagag acgttgtcag gtacaattct ttcctttatc gtcctcatgg caaaccatga
     8881 agatttccaa gaccgtgcat atgcagaaat tctgtctgtt gtcggcacag ataggtttcc
     8941 aaattgtcga gaccgcccat cgctcatatt tgtggatgca cttatccgag aagtataccg
     9001 attcaatcct atcgtacccg tgatacaccg tagcaactac gaagaggagg aatatgaaga
     9061 acattttatt cccaagaaga cttggatcct cgtcaacctg tggtcgattt tacatgatcc
     9121 taacgtctat ccatcgccag aaacattctg tcccgaacgt tttctcaact ccgataaaga
     9181 catcaatccc gatccccgct cttttgttta tggttttgga cgaagaaggt gtcccgcagt
     9241 ggatttcgcg gatacaacac tttttattac cataactcgc cttctgatgg tcttcaaaat
     9301 ttccccggag atcgttgatg ggattgcata cccgcccctg ctggagatta tggaaggatt
     9361 tactccggct ccaaaaccgt tccgatgtaa attcatatct cggcaggaca gcaagcgctt
     9421 gctattcgat ccatttgaca gtaccacaga taattgaatt taactcctta agttacttta
     9481 atgatttagt ttttattatt aataattcat gctcatgaca tctcatatac acgtttataa
     9541 aacttaaata gattgaaaat gtattaaaga ttcctcaggg attcgatttt tttggaagtt
     9601 tttgtttttt tttccttgag atgctgtagt atttgggaac aattatacaa tcgaaagata
     9661 tatgcttaca ttcgaccgtt ttagccgtga tcattatcct atagtaacat aacctgaagc
     9721 ataactgaca ctactatcat caatacttgt cacatgagaa ctctgtgaat aattaggcca
     9781 ctgaaatttg atgcctgaag gaccggcatc acggattttc gataaagcac ttagtatcac
     9841 actaattggc ttttcgctag tcttatctaa aaattgcctt tatagtccgt ctctccagtc
     9901 acggcctgtg taactgatta atcctgcctt tctaatcacc attctactgt ttaattaagg
     9961 gattttgtct tcatcaacgg cttccgccca aaaaaaagta tgacgttttg cccgcaggcg
    10021 tgaagctgcc catcttcacg ggcctgacct cctctgccgg aacaccggcc atctccaact
    10081 cataaattgg agaaataaga gaatttcaga ttttcagagg atgaaaaaaa aaaggtagag
    10141 agcataaaaa tggggttcac tttttggcaa agttacagta tgcttattac atataaatag
    10201 agtgccgata atggcttttt ttcatcttcg aaatacgctt gctactgctc ttccagcgtt
    10261 tttattactt ctttcttgtt tctccttagt atataaaata tcaagctaca acaagcatac
    10321 aatcaactgt caactgtcaa ttatattata atacactatg agtacctctt taactaacta
    10381 cgctcttccg gacagtagga tcgaggttgt gcggcttaac cagcaatata tcaggttcaa
    10441 gaaacttttc ggacgtaccg gaccatttcc gacaactgtc gacgttgacg gcatcgacca
    10501 tgttctcgag gttgcggccg gaacatgtgc ttggactctg gatatcgcac atcttcaaga
    10561 agtctacacc cgcctcccat cgatgccatc atcgactcgt cctgagtcta cgtcaaagcc
    10621 aattcagttg tacgcctgcg atattagcac agataagttt cccccgaggg acgtgacgga
    10681 tgcgctcggt atcaagacgt ttcaacagga cgttacacaa ccctttcccg acgagcttta
    10741 cggaaagttt gacctcgttc aagcgtttat gcttgtcggt tctttaacag aggctgggtg
    10801 gaggagggct ctggagaact accgcagact tctcaaaccc ggagggtttc tcttcatgct
    10861 cgaaacggat gtggccctgt attcaacgag cctcccacca ccaccaccac cgcttttccc
    10921 ccttgacatc gacggtgtta tggacggcct cacgtggatt cacagtgcga atcggttgtt
    10981 caccgggttc aacataaacc gggacctggt tcctaatata tcgtcccggt tgccggcgct
    11041 gttaacggag cagtctctca acatcataca agaagctaag gctatctcac cggttggaaa
    11101 gctctgcaaa ttataccgcg cgttggacgg cgcagattta tctgatgatt gggatgactc
    11161 tgttcagggc actcgggatg tttttcgtgg attggctcag tcaatgctta gaagaagcat
    11221 gttggaggta ccaaaaggaa cacccatcac gactcaggaa ggtctgaatg agcttctcgc
    11281 ttggatattt caggggttgg aatcggatgg ggcttgtcat gtgatgacac agtggataat
    11341 aagaaggaac tgagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
    11401 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
    11461 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
    11521 tattgaccac acctctaccg gcatgcctct caaatctttt agcgccaagg actccaacta
    11581 attgtatctt gaatttgcct ttacgatccg tttgtccagt cacggcatgt atatcttatt
    11641 aatcctgcct ttctaatcac gtattctaat gttcaattaa gggattttat cttcatcaac
    11701 ggctcccacg caaaaaatga cgttttgcac acagacacga aatacacctt ccaccggaac
    11761 aacggccatc tccaacttat aagttgggga aataagacaa tttcagactt cagagaatga
    11821 aaaaaaaaaa ggtacatcac agatggggtt caggtttgct acaattgcag ggagcctgtc
    11881 acatataaat agacctccag tgatgatatc tttcagtctt caaacgtctc ttgtcacagt
    11941 tctggtcgtt ctatatcaca tctctcttgg ttctacttat tgtctataat atcaagctac
    12001 agcaagcata caatcaacta tctaccatac cataatacac aatgaactct gaggataccg
    12061 ccgctgacca tggaccaccc agtatctcga aagctgaaga aggattggaa tctactgggc
    12121 cttctggaga aacgcccgct tctgcgctca tctctgactt tcctgaaggc ggtcgcgacg
    12181 gatggttgac cgtgttggga ggtgctcttg ttctcttttc ttcattcggt gccgccctct
    12241 ccttcggagt ctttcaagac tattacactc gcatttctct aaatgaacac tccgcgtcgc
    12301 aaatatcctg gatcggatcc actcagctgt tccttgattt tgctgtcggc ctgcccgcag
    12361 ggaaactctt cgacgaaggt catttcaggc ttcttatgat ttcaggaact gtcttataca
    12421 gcttctcgtt cttcatgctt tcacttgcca aaccgcataa ctactaccaa gtctttctgt
    12481 cgcaggccat tggtatggga ctcggaatgg ggctagtcat catccctgct gtctcgatac
    12541 cattgcatta ctttaggcgg aggagattac ttgctatggg attcatattt gcgggctcct
    12601 ctcttggcgg tgtcattttc cctatcatgc tcaacaagct tatcaacggc cgtgctgggt
    12661 tcgcgtgggg aataagggct gccgctttct tgtcgctcgg gctcttgatc atcgcaaatt
    12721 tgattatgaa gcctaggcta cccctcaaaa aggatcgatc gcctgatact gtggtaccgt
    12781 tgaagagcat attgaccgat gtcgcatacc taatcagtgt ctttggagcc ttcctgattt
    12841 tttgggggct tttcttccct ttcttctatc tccagctgtt ttcggtactt cacggagtag
    12901 acgaaaagct tgctttttac tcgttgacgg ttctcaatgc gtccacaatc tttggtcgca
    12961 caattccgaa tttcgcatcc gataaatttg gacctttgaa cgttgcgatc ccgatgacta
    13021 ccatcgccgg cgggctcgta tttgcgatgc tgggcgccac aagtgtcggc ggaatgttca
    13081 cattcgcagt cgtctatgga ttcttcggtg gagcatgtgc ctcccgcatc ggtatcgcaa
    13141 tgtttgtctg cggatttgca atcctcaccg gaacacccat caccggggca ctgctcaaac
    13201 cgcctcagta caacacctgg acgaagcccg tcatctttag cgcggtggtc atcttggctg
    13261 gggtcggatg cctggtgata tcaaggcaga tgacggtgaa gaggaagggg acgtggaaag
    13321 tgtgaggaga ttgataagac ttttctagtt gcatatcttt tatatttaaa tcttatctat
    13381 tagttaattt tttgtaattt atccttatat atagtctggt tattctaaaa tatcatttca
    13441 gtatctaaaa attcccctct tttttcagtt atatcttaac aggcgacagt ccaaatgttg
    13501 atttatccca gtccgattca tcagggttgt gaagcatttt gtcaatggtc gaaatcacat
    13561 cagtaatagt gcctcttact tgcctcatag aatttctttc tcttaacgtc accgtttggt
    13621 cttttatagt ttcgaaatct atggtgatac caaatggtgt tcccaattca tcgttacggg
    13681 cgtatttttt accaattgaa gtattggaat cgtcaatttt aaagtatatc tctcttttac
    13741 gtaaagcctg cgagatcctc ttaagtatag cggggaagcc atcgttattc gatattgtcg
    13801 taacaaatac tttgatcggc gctatgcggc cgccaccgcg gtggagctcc agcttttgtt
    13861 ccctttagtg agggttaatt gcgcgcttgg cgagactcaa acaagatata ttttgcccgc
    13921 cttacagacg aaactagttg gaggttatgg agcatactat cacgtgggcg gccactggtg
    13981 agttactaca ccccaggggc aacgttgatg ctcctaaaaa actctggctg gacgcaagcc
    14041 gtaacacccg tgtcacttca taatcgtttg caattcaggg cttgatctac acattaccct
    14101 gttatcccta ttaattaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    14161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    14221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    14281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    14341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    14401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    14461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    14521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    14581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    14641 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    14701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    14761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    14821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    14881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    14941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    15001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    15061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    15121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    15181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    15241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    15301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    15361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    15421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    15481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    15541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    15601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    15661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    15721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    15781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    15841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    15901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    15961 tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
    16021 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
    16081 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
    16141 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
    16201 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
    16261 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
    16321 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
    16381 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
    16441 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
    16501 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
    16561 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
    16621 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
    16681 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
    16741 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
    16801 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
    16861 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
    16921 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
    16981 aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc
    17041 actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac
    17101 ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa
    17161 aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct
    17221 tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc
    17281 ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca
    17341 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    17401 ggtgagcgcg tttcggtgat gacggtgaaa acctcggcgc gccattaccc tgttatccct
    17461 aatatacaac gcaaagaagc tggaaaattg gcgagggaat cctgtttctg tctatccaag
    17521 aatgggcatg aggtggcaac cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg
    17581 gacaccggac actcgctgtt ttcgaaatta ccctttaagc gcgggtattg aaccaggctt
    17641 atgcccaaga tcgtagcaag catttaaatg acggtatcga taagcttgat atcgaattcc
    17701 tgcagcccgg gggatccact agttctagat taattaatat ctaaaaattg ccttatgatc
    17761 cgtctctccg gttacagcct gtgtaactga ttaatcctgc ctttctaatc accattctaa
    17821 tgttttaatt aagggatttt gtcttcatta acggctttcg ctcataaaaa tgttatgacg
    17881 ttttgcccgc aggcgggaaa ccatccactt cacgagactg atctcctctg ccggaacacc
    17941 gggcatctcc aacttataag ttggagaaat aagagaattt cagattgaga gaatgaaaaa
    18001 aaaaaaaaaa aaaaaggcag aggagagcat agaaatgggg ttcacttttt ggtaaagcta
    18061 tagcatgcct atcacatata aatagagtgc cagtagcgac ttttttcaca ctcgaaatac
    18121 tcttactact gctctcttgt tgtttttatc acttcttgtt tcttcttggt aaatagaata
    18181 tcaagctaca aaaagcatac aatcaactat caactattaa ctatatcgta atacacaatg
    18241 ct
//
