LOCUS       sequence-224242-       10983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TurboID under Gal4/UAS control. TurboID is tagged at the 
            N-terminus with V5 and a nuclear export signal (NES) at the 
            C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, 
            Feldman JL, Perrimon N, Ting AY
  TITLE     Efficient proximity labeling in living cells and organisms with 
            TurboID.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
  PUBMED    30125270
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201."
COMMENT     Sequence Label: pUAS-V5-TurboID-NES
FEATURES             Location/Qualifiers
     source          1..10983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1447..2061,2132..2263,2467..2782,2844..3498,
                     3573..3846,4248..4319))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4771..4789
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4912..4981
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5219..5649
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5667..5700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5713..5807
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5823..5856
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5869..5963
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5987..6225
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6172..6193
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6271..6295
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6319..6360
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6367..7323
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             7330..7356
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(7382..7406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7713..7778
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7908..7928
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8200..8321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8237..8256)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8291..8310
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8343..8773
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(8792..8812)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8792..8810)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8831..8847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8831..8847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8844..8866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8855..8871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8879..8885,8886..8903,8904..8909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8924..8945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9062..9079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9233..9821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9313..9332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9992..10783,10784..10852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10853..10957)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtacaagct
      661 tcacgaccta ggcgcgccct agttccagtg aaatccaagc attttctaaa ttaaatgtat
      721 tcttattatt atagttgtta tttttgatat atataaacaa cactattatg cccaccattt
      781 ttttgagatg catctacaca aggaacaaac actggatgtc actttcagtt caaattgtaa
      841 cgctaatcac tccgaacagg tcacaaaaaa ttaccttaaa aagtcataat attaaattag
      901 aataaatata gctgtgaggg aaatatatac aaatatattg gagcaaataa attgtacata
      961 caaatattta ttactaattt ctattgagac gaaatgaacc actcggaacc atttgagcga
     1021 accgaatcgc gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg
     1081 ttgcggagag cgggtgggag acagcgaaag agcaactacg aaacgtggtg tggtggaggt
     1141 gaattatgaa gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt
     1201 ttatgtagcg ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt cttttggtta
     1261 gaagacaaat ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt gtattctata
     1321 ggtcatatct tgtttttatt ggcacaaata taattacatt agctttttga gggggcaata
     1381 aacagtaaac acgatggtaa taatggtaaa aaaaaaaaac aagcagttat ttcggatata
     1441 tgtcggctac tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa
     1501 gctcacgatg agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     1561 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     1621 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     1681 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
     1741 gaagccgcca aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga
     1801 ggtcgaggag ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag
     1861 agtgaccagc gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg
     1921 ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt
     1981 tttgcccaga aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac
     2041 tggcagctct gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt
     2101 gaaatgcaaa tgtattctaa acatgactta catttatcgt ggcaaagacg ttttgaaagg
     2161 tcatgttggt caggaagagg aagatggctc cgttgatatt catcacaccc acttgcgtga
     2221 gttgttggcc caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt
     2281 actcgcatct cattaatatt cgcgagttaa atgaaattta tttatcttct gcaaaactat
     2341 aaactataca tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa
     2401 aatctagcga atttgtttcc aagaattgta agcgttatat catttgtttc cactggaacc
     2461 actcaccgtt gtctgaataa gtcgcacttt tacgaggagt ggttccttga gcaccgacag
     2521 ccaggatcgc cacaggaccg cccggaactg catgaaccag gtggccttgt aggtgtaccc
     2581 attctccggc tgctccagtg gcttctccag atttttggtg gccaacaact gctccatatc
     2641 ccgggctact ttgctaatgg caaaattgtc gcatatcttg gcgatccgat cacgggactc
     2701 gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg gattgtagtt
     2761 ggtaggacac tgggcaccca cgctggatag gagttgagat gttatgtaat actagatacc
     2821 cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg ggagtgccca
     2881 agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca aacagctcgg
     2941 aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc ttcttcagca
     3001 cctggacgac gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca tcgcagatca
     3061 gaagcggcgg atcggttaga gcctcggagg cgaatgccag acgcttcctt tctccgccgg
     3121 acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa
     3181 gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca
     3241 tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt tagggagccg ataaagaggt
     3301 catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca ggttggccat
     3361 tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga aaggcaaggg
     3421 cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa agttcgcccg
     3481 gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat taagatttca
     3541 cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt tcctgggcgc
     3601 cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc
     3661 ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat aggtgaggtt
     3721 ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg gccggagcgt
     3781 gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag ccgctccgct
     3841 gtcaccctgc aatgataggg gatctcaaat atcaactaca agcgttatgc tcatctaacc
     3901 ccgaacaaaa agtaccccga agtatcctac gaagtaggtt tatactttta tttatttttt
     3961 gtgcatctag gatcagctta aaatatctgg ttgttatatt ttttgtaaaa aagaatatag
     4021 tcgaaaatga atgcctttag atgtcttgat catgatatga tctcaaaaat tgtcttatat
     4081 agcgagaaca gctaccagaa taatctgttt cgtgtcacta tttgtttgtg caattgcggt
     4141 ttgggatttt tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg caatcgcagt
     4201 tcctatagat caagtgaact taagatgtat gcacatgtac tactcacatt gttcagatgc
     4261 tcggcagatg ggtgtttgct gcctccgcga attaatagct cctgatcctc ttggcccatt
     4321 gccgggattt ttcacacttt cccctgctta cccacccaaa accaatcacc accccaatca
     4381 ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg gcacagcact ttgtgtttaa
     4441 ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg
     4501 cggacattga cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc
     4561 gaaagagacg gcgatatttc tgtggacaga gaaggaggca aacagcgctg actttgagtg
     4621 gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata cccttggatc
     4681 gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta aaaaaaaaaa
     4741 atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata catactaggc
     4801 gcgccagatc gcaagaagct tgatatcatc gatctcgagg ctgcatccaa cgcgttggga
     4861 gctctccgga tcaattcggc ttcaggtacc gtcgacgatg taggtcacgg tctcgaagcc
     4921 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     4981 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     5041 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     5101 tgcgacggcg tcggctggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     5161 ggcgggcatg tcgacaagcc gaattgatcc actagaaggc ctaattcggt acactagttg
     5221 gccacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt
     5281 aaaattttgt tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa
     5341 taatttttta ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
     5401 aatattgcat actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat
     5461 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaga atattgcata
     5521 cgttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt
     5581 tcttgccata ccatttagcc gatcaattgt gctcggcaac agtatatttg tggtgtgcca
     5641 accaacaaca ctagtagtac cagcttataa cttcgtataa tgtatgctat acgaagttat
     5701 ctgcaggcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     5761 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactcccat
     5821 ggataacttc gtataatgta tgctatacga agttatggat ccgcaggtcg gagtactgtc
     5881 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     5941 cgagcggagt actgtcctcc gagcggagac tcgtcgacag cgagcgccgg agtataaata
     6001 gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta
     6061 agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt
     6121 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
     6181 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga
     6241 attcgcagat ctgcggccgc ggctcgaatc acaagtttgt acaaaaaagc aggctccgcg
     6301 gccgccccct tcaccatggg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     6361 gctagcaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa tggcgagttc
     6421 catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat caacaagcac
     6481 attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa gggctactct
     6541 ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg
     6601 agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga tcgaatcggc
     6661 gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga
     6721 gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg
     6781 aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg
     6841 ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag
     6901 gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag
     6961 atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat
     7021 cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct
     7081 acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca
     7141 tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt
     7201 ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg
     7261 gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa
     7321 aagctgcagc tgcctcccct ggagcgcctg accctggact aatagaaggg tgggcgcgcc
     7381 gacccagctt tcttgtacaa agtggtgatt cgagggtacc tctagagcaa actagttctg
     7441 atctgctaga caattgttgg catcaggtag gcatcacaca cgattaacaa cccctaaaaa
     7501 tacactttga aaatattgaa aatatgtttt tgtatacatt tttgatattt tcaaataata
     7561 cgcagttata aaactcatta gctaacccat tttttctttg cttatgctta cagattgcaa
     7621 agaactagag ccgcgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     7681 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     7741 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     7801 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     7861 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     7921 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     7981 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     8041 tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata
     8101 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     8161 ctgaacctga aacataaaat gaatggaatt gttgttgtta acttgtttat tgcagcttat
     8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gttccagagc
     8341 tctggccacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat attttcggca
     8401 aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt ttagagaaac
     8461 caaataattt tttattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     8521 aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     8581 atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata aagaatattg
     8641 catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac
     8701 cttttcttgc cataccattt agccgatcaa ttgtgctcgg caacagtata tttgtggtgt
     8761 gccaaccaac aacgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     8821 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8881 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8941 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9001 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9061 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9121 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9181 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9421 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9541 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9601 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9661 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9721 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9781 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9841 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9901 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9961 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10021 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10081 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10141 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10201 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10261 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10321 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10381 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10441 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10501 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10561 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10621 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10681 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10741 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10801 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10861 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10921 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10981 ctg
//
