LOCUS       sequence-224244-       10797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged 
            at the N-terminus with V5 and a nuclear export signal (NES) at the 
            C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, 
            Feldman JL, Perrimon N, Ting AY
  TITLE     Efficient proximity labeling in living cells and organisms with 
            TurboID.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
  PUBMED    30125270
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201."
COMMENT     Sequence Label: pUAS-V5-miniTurboID-NES
FEATURES             Location/Qualifiers
     source          1..10797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1447..2061,2132..2263,2467..2782,2844..3498,
                     3573..3846,4248..4319))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4771..4789
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4912..4981
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5219..5649
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5667..5700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5713..5807
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5823..5856
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5869..5963
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5987..6225
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6172..6193
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6271..6295
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6319..6360
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6367..7137
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             7144..7170
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(7196..7220)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7527..7592
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7722..7742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8014..8135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8051..8070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8105..8124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8157..8587
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(8606..8626)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8606..8624)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8645..8661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8645..8661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8658..8680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8669..8685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8693..8699,8700..8717,8718..8723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8738..8759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8876..8893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9047..9635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9127..9146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9806..10597,10598..10666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10667..10771)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtacaagct
      661 tcacgaccta ggcgcgccct agttccagtg aaatccaagc attttctaaa ttaaatgtat
      721 tcttattatt atagttgtta tttttgatat atataaacaa cactattatg cccaccattt
      781 ttttgagatg catctacaca aggaacaaac actggatgtc actttcagtt caaattgtaa
      841 cgctaatcac tccgaacagg tcacaaaaaa ttaccttaaa aagtcataat attaaattag
      901 aataaatata gctgtgaggg aaatatatac aaatatattg gagcaaataa attgtacata
      961 caaatattta ttactaattt ctattgagac gaaatgaacc actcggaacc atttgagcga
     1021 accgaatcgc gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg
     1081 ttgcggagag cgggtgggag acagcgaaag agcaactacg aaacgtggtg tggtggaggt
     1141 gaattatgaa gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt
     1201 ttatgtagcg ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt cttttggtta
     1261 gaagacaaat ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt gtattctata
     1321 ggtcatatct tgtttttatt ggcacaaata taattacatt agctttttga gggggcaata
     1381 aacagtaaac acgatggtaa taatggtaaa aaaaaaaaac aagcagttat ttcggatata
     1441 tgtcggctac tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa
     1501 gctcacgatg agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     1561 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     1621 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     1681 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
     1741 gaagccgcca aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga
     1801 ggtcgaggag ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag
     1861 agtgaccagc gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg
     1921 ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt
     1981 tttgcccaga aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac
     2041 tggcagctct gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt
     2101 gaaatgcaaa tgtattctaa acatgactta catttatcgt ggcaaagacg ttttgaaagg
     2161 tcatgttggt caggaagagg aagatggctc cgttgatatt catcacaccc acttgcgtga
     2221 gttgttggcc caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt
     2281 actcgcatct cattaatatt cgcgagttaa atgaaattta tttatcttct gcaaaactat
     2341 aaactataca tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa
     2401 aatctagcga atttgtttcc aagaattgta agcgttatat catttgtttc cactggaacc
     2461 actcaccgtt gtctgaataa gtcgcacttt tacgaggagt ggttccttga gcaccgacag
     2521 ccaggatcgc cacaggaccg cccggaactg catgaaccag gtggccttgt aggtgtaccc
     2581 attctccggc tgctccagtg gcttctccag atttttggtg gccaacaact gctccatatc
     2641 ccgggctact ttgctaatgg caaaattgtc gcatatcttg gcgatccgat cacgggactc
     2701 gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg gattgtagtt
     2761 ggtaggacac tgggcaccca cgctggatag gagttgagat gttatgtaat actagatacc
     2821 cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg ggagtgccca
     2881 agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca aacagctcgg
     2941 aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc ttcttcagca
     3001 cctggacgac gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca tcgcagatca
     3061 gaagcggcgg atcggttaga gcctcggagg cgaatgccag acgcttcctt tctccgccgg
     3121 acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa
     3181 gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca
     3241 tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt tagggagccg ataaagaggt
     3301 catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca ggttggccat
     3361 tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga aaggcaaggg
     3421 cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa agttcgcccg
     3481 gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat taagatttca
     3541 cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt tcctgggcgc
     3601 cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc
     3661 ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat aggtgaggtt
     3721 ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg gccggagcgt
     3781 gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag ccgctccgct
     3841 gtcaccctgc aatgataggg gatctcaaat atcaactaca agcgttatgc tcatctaacc
     3901 ccgaacaaaa agtaccccga agtatcctac gaagtaggtt tatactttta tttatttttt
     3961 gtgcatctag gatcagctta aaatatctgg ttgttatatt ttttgtaaaa aagaatatag
     4021 tcgaaaatga atgcctttag atgtcttgat catgatatga tctcaaaaat tgtcttatat
     4081 agcgagaaca gctaccagaa taatctgttt cgtgtcacta tttgtttgtg caattgcggt
     4141 ttgggatttt tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg caatcgcagt
     4201 tcctatagat caagtgaact taagatgtat gcacatgtac tactcacatt gttcagatgc
     4261 tcggcagatg ggtgtttgct gcctccgcga attaatagct cctgatcctc ttggcccatt
     4321 gccgggattt ttcacacttt cccctgctta cccacccaaa accaatcacc accccaatca
     4381 ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg gcacagcact ttgtgtttaa
     4441 ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg
     4501 cggacattga cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc
     4561 gaaagagacg gcgatatttc tgtggacaga gaaggaggca aacagcgctg actttgagtg
     4621 gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata cccttggatc
     4681 gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta aaaaaaaaaa
     4741 atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata catactaggc
     4801 gcgccagatc gcaagaagct tgatatcatc gatctcgagg ctgcatccaa cgcgttggga
     4861 gctctccgga tcaattcggc ttcaggtacc gtcgacgatg taggtcacgg tctcgaagcc
     4921 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     4981 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     5041 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     5101 tgcgacggcg tcggctggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     5161 ggcgggcatg tcgacaagcc gaattgatcc actagaaggc ctaattcggt acactagttg
     5221 gccacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt
     5281 aaaattttgt tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa
     5341 taatttttta ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
     5401 aatattgcat actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat
     5461 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaga atattgcata
     5521 cgttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt
     5581 tcttgccata ccatttagcc gatcaattgt gctcggcaac agtatatttg tggtgtgcca
     5641 accaacaaca ctagtagtac cagcttataa cttcgtataa tgtatgctat acgaagttat
     5701 ctgcaggcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     5761 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactcccat
     5821 ggataacttc gtataatgta tgctatacga agttatggat ccgcaggtcg gagtactgtc
     5881 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     5941 cgagcggagt actgtcctcc gagcggagac tcgtcgacag cgagcgccgg agtataaata
     6001 gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta
     6061 agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt
     6121 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
     6181 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga
     6241 attcgcagat ctgcggccgc ggctcgaatc acaagtttgt acaaaaaagc aggctccgcg
     6301 gccgccccct tcaccatggg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     6361 gctagcatcc cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg
     6421 gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg
     6481 aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg
     6541 aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg
     6601 ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga
     6661 aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga
     6721 aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc
     6781 attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc
     6841 tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg
     6901 atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg
     6961 tcacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac
     7021 aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag
     7081 gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg
     7141 cagctgcctc ccctggagcg cctgaccctg gactaataga agggtgggcg cgccgaccca
     7201 gctttcttgt acaaagtggt gattcgaggg tacctctaga gcaaactagt tctgatctgc
     7261 tagacaattg ttggcatcag gtaggcatca cacacgatta acaaccccta aaaatacact
     7321 ttgaaaatat tgaaaatatg tttttgtata catttttgat attttcaaat aatacgcagt
     7381 tataaaactc attagctaac ccattttttc tttgcttatg cttacagatt gcaaagaact
     7441 agagccgcgg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     7501 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     7561 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     7621 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     7681 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     7741 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     7801 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     7861 acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc
     7921 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     7981 ctgaaacata aaatgaatgg aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8041 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8101 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggttcca gagctctggc
     8161 cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc ggcaaagtaa
     8221 aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag aaaccaaata
     8281 attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa
     8341 tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg
     8401 catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat attgcatacg
     8461 ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc ataccttttc
     8521 ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac
     8581 caacaacgag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     8641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
