LOCUS       sequence-224266-       17001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17001)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 17001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU16.1-2.2
FEATURES             Location/Qualifiers
     source          1..17001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1043..1342
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3927..4147
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4148..4951
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(12682..12702)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12682..12700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(13039..13627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13119..13138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13798..14589,14590..14658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14421..14440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14659..14763)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14790..16132
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(15753..15800)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     16173..16191
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16533..16549)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16533..16549)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 caagctacaa aaagcataca ataactatca actattaact atatcgtaat acacaatggc
       61 cacgtactct agccgtcttc cttctcctct caagcctttg gaacacatct ggcgtacggc
      121 atacgctttt acgaagtctg actacaagac agtcgtcttc ccagtcatct gctatgggat
      181 aatggctgct ccacagatgt ccatcaccag acttcctcac ctcgcggtat gggtgtggct
      241 tcaccttctg cagttctgtg cagcgaatca ggcgttgagc cctgaagaag acgcattgaa
      301 taagccgtat agacccatcc cagcgggctt aatttcgatt cggggtgcca gagtccttcg
      361 ttggctcctc gtcccggtct gcctggcgct ctcgtggacg ctggacgtta tctacccagg
      421 gatcagtcta gccatctcgt tcgtcgtcta caacgagctc ggcttggata actactggta
      481 tacgaagaac ttcctcaacg ctgtgggcct cgtctcctgg aatatcggcg cggccaagat
      541 cgcatgtgca gagaatgttt ccctcgacca gcgggcctgg tttgcgcctt acgttagcac
      601 gcttcttatc gctacgacga tacatatcca agacttccgc gatgaggcag gagataggaa
      661 acagggacga gtcacattcc ccgtcgtgat gcccgaattc tctaggcgca tgacattcct
      721 catcgtcgca gtgtggtcgt tcggcctcgc gtcgtattgg aacctaagca gcgtcgcatc
      781 tccgtggttc gtcacactgg ggatgtacgt tgccctcaga gtgctccagc agcgcaccga
      841 gcacgaagat aaggtctcat tgcggatata catggtacgt tgtcatcccc cgaacgcacc
      901 cacttcatcc ccgcctaacc cgataacctg ttcagttctg gctgtgcgtt gctcaaatcc
      961 tccccttctg gacaaggcaa atggagggaa tagcccagcc gtggctgcca aatgctcagt
     1021 tcggggctat tcagaacaat gagcggatct cttatgtctt tacgatttat agttttcatt
     1081 atcaagtatg cctatattag tatatagcat ctttagatga cagtgttcga agtttcacga
     1141 ataaaagata atattctact ttttgctccc accgcgtttg ctagcacgag tgaacaccat
     1201 ccctcgcctg tgagttgtac ccattcctct aaactgtaga catggtagct tcagcagtgt
     1261 tcgttatgta cggcatcctc caacaaacag tcggttatag tttgtcctgc tcctctgaat
     1321 cgtctccctc gatatttctc attttccttc gcatgccagc attgaaatga tcgaagttca
     1381 atgatgaaac ggtaattctt ctgtcattta ctcatctcat ctcatcaagt tatataattc
     1441 tatacggatg taatttttca cttttcgtct tgacgtccac cctataattt caattattga
     1501 accctcacga tccagttctc cagtgacaca gcctttatct ggtcaaacct ttctttctaa
     1561 tcacctatgc tgatgcttaa ttaagggatt tttgtctcca tcaacggcat gcgcccaaaa
     1621 atgacgtttt ttttaaccca tagacacgaa actacccatt ttccaccggc ctgacctacc
     1681 accggaacaa cggccatctc caacttgcaa gttggggaaa ttaagagcat cgcaggttta
     1741 atggaagaaa aaaaaaaggt acagcacagc gcaaatggag ttagttccct tatgtcacac
     1801 actcacacac agtcggtcag atcaagcata ctgggtgcgt ataaatagag tggccattgc
     1861 caccctgttt atctcaaaat ctgtcttgtt agtggtcttc tccctttttc aggttacaat
     1921 tctcttgttt ctacttagta tataagtata tcaagctata ttaagcatac tatcaactgt
     1981 caactctatc ctcaaaatac aatacaaaat gatgcgtact tcgtttgcaa tcctcgcggc
     2041 gattatcccg ctaacattca cgtttggtgc ttcggtagag aatcttctgc ccagaaacac
     2101 cacccaaaag caagacctta caccagctca atgctgcgac ggcctgaagc gtatcttaga
     2161 cccttctaaa gtccttctgc caggctcccc tgagtatgcg gctcaggagg cctcctatta
     2221 ctccggcgaa caggtcctac agttccctgt ctgccgagtc gcacccacca gtcccgaaga
     2281 cgtctcaagt ataatcaaat ttgctaccac aagtaactgc aattttgccg tacgttcggg
     2341 cggtcatatg aactgggttg gttcatcgaa catcggtccc gaagggttta ctatcgactt
     2401 ggaggacatg aagcagattg agcttctgga cgattcgaag actgtcgcta tctccccggg
     2461 attgacctgg gccgatgtct ataccttctt acgtccccac aatctgcata ctgccggggg
     2521 ccggtcgtcc cacgtcggtg taggtggctt ccttcttgga ggtggcatct cattcctctc
     2581 cctcgagcac ggtttcggaa gcgacaacat catcaactac caaatcgtct tagccgatgg
     2641 ctcgatcgtc aacgccaatg aagccgagcg aagcgacctg tactgggctc tcaaaggtgg
     2701 tagcacgaac tacggcatag ttacccgctt tgacatggcg acgttcccgt tggatcaaat
     2761 gtggggagga tctctctact acaacatcag cctgggccct accctcctcg attatctcgt
     2821 cgggtttacg gagagactcg ccgtcgatgc caatggccta ttcgcgctgt ctatcgcttg
     2881 gaacgcagcc aataatgatt acctcatttg gtcaccgaac gcctacctcg cacctacacc
     2941 cttcccaagc ccactctacg atggactcga ggaaatcccg acggtgcgcg ataccatgcg
     3001 catcacaaac ctcgtatctg tcacagacga aatcaatatg tcatccccag gcggaagccg
     3061 aactcagtgg ttcgctttgg tgttcaaggc agacgcgacc ctgccttggg atatctatca
     3121 gaagggcgct gatattatgg cgccgttcct tgggaaggag gggacctcgt gggcgctcac
     3181 gttacaaccc attaatgtcg gcatgatcgc tgctgctgcg aatcgtggag gaaacccatt
     3241 cggtctcacc gaaaaggatg gcgatctatt ccttcttttg atatccatct tctggactga
     3301 cccagaggac gacggaaaga tcaaggctac gacgcaagac ttcctgcgat ggtcgacgga
     3361 cactgccaag cagcgcaacc ttctcaatcg tttcatctac ttgaactacg cgttggatac
     3421 gcagccagtc ttagcgagtt tcggggacga gaacgtcagg cgtatgaagg ccgtgcagaa
     3481 gaagtacgat ccaaagggtc tgctcaaagt gtggaaaggt ggatacaagc ttgacgaagt
     3541 atagacaaat cgctcttaaa tatataccta aagaacatta aagctatatt ataagcaaag
     3601 atacgtaaat tttgcttata ttattataca catatcatat ttctatattt ttaagatttg
     3661 gttatataat gtacgtaatg caaaggaaat aaattttata cattattgaa cagcgtccaa
     3721 gtaactacat tatgtgcact aatagtttag cgtcgtgaag actttattgt gtcgcgaaaa
     3781 gtaaaaattt taaaaattag agcaccttga acttgcgaaa aaggttctca tcaactgttt
     3841 aaaaggagga tatcaggtcc tatttctgac aaacaatata caaatttagt ttcaaagatg
     3901 aatcagtgcg cgaaggacat aactcattca attcaattca tcattttttt tttattcttt
     3961 tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
     4021 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
     4081 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     4141 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
     4201 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
     4261 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
     4321 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
     4381 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
     4441 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
     4501 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
     4561 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
     4621 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
     4681 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
     4741 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
     4801 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
     4861 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     4921 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     4981 actaaactca caaattagag cttcaattta attatatcag ttattaccct atgcggtgtg
     5041 aaataccgca cagatgcgta aggagaaaat accgcatcag gataggaaaa aaccgagctt
     5101 cctttcatcc ggcgcggctg tgttctacat atcactgaag ctccgggtat tttaagttat
     5161 acaagggaaa gatgccggct agactagcaa gttttaggct gcttaacatt atggataggc
     5221 ggataaaggg cccaaacagg attgtaaagc ttagacgctt ctggttggac aatggtacgt
     5281 ttgtgtatta agtaaggctt ggctggggat agcaacattg ggcagagtat agaagaccac
     5341 aaaaaaaagg tatataaggg cagagaagtc tttgtaatgt gtgtaacttc tcttccatgt
     5401 gtaatcagta tttctactta cttcttaaat atacagaagt aagacagata accaacagcc
     5461 tttcccagat atacatatat atctttattt cagcttaaac aataattata tttgtttaac
     5521 tcaaaaataa aaaaaaaaaa ccaaactcac gcaactaatt attccataat aaaataacaa
     5581 catgagcgcg aatgtatcat ggatccccac agcaccggcg acgttctggt cttcaccaaa
     5641 cgaccaattc tctacatggc ctaagctcgc tgtgttggcg ttatccacct tcatcattca
     5701 tcgactgtac aagtcggcgt cgttgaacat gaagctgccc cccgggccaa gggggtggcc
     5761 cgttattggg agtgtattcg atattggacc acatatgtgg ctcacgttca cagagtggaa
     5821 gaagcaatat ggaccgattt tctatgtgaa cctcgcaggg cgttcgatga tcgtcttgaa
     5881 cacccacgaa gtcgccacgg agctccttga taagaggtcg tccatctact cagacagacc
     5941 ccgccatatc gtcgcctctg agatcatgtc aggcgaatac ctactcggat tcatgcattt
     6001 cgacgataag tggaagcgtg tcaggagagg ttctcatgag gcgatgaaca accaaatcac
     6061 caaaacctac cgtccgttca tgcaagtcga aggatacctc ctcgccgaca gcctcatgaa
     6121 agcgccttct atgtggaacg atcacatcaa gcgttctgcg gcatctttca tcatgtccgt
     6181 cgtatacggc accaagtcca tgcgcacctc caccgacccc accctcatca ccctccacaa
     6241 gtttctgagg agatcgctcg gcgcggccgc tgggaagtac tgggtcgagt tcttccactt
     6301 tttggagtac atgccaaggt ggttggctcc gtggagggtg gaggccgagg agtggttcaa
     6361 gacggacagc aagatgttcg aggggttcta caaccaagtt aagaagagga tggccgacgg
     6421 agacaacagg ccgtgcacag ttacgactct tatcgaagaa caaaaaaaga aaaacctctc
     6481 tactatggaa tcagtctggc tggcagcggc tatgtacggt ggaggctccg aatcaactgc
     6541 tggatccctc ggctggtgga taactgccat ggtagtctac ccctccgccc tccgcgccgc
     6601 ccaagaagag ctcgatcgcg tcgtaggccg agatcgtatg cccaccttcg acgactatga
     6661 ccacctcccc tactgcaggg ccatggtcaa ggaggtcttg aggtggcgac ccgcaacccc
     6721 cctcggtgtc ccccatcgcg tggaaaggga tgactggttc aacggttact tcatcccgaa
     6781 gggaacattg gttgtggcga acatttgggg catgaaccac gacgagaaag tctacgggcc
     6841 cgacgttcac gagttcaacc cggcccgttt ccttgaagga tctggaaagc ccctgccctc
     6901 tgtctcagat accaaggacg agggccatgt tacctacggc ttcggccggc gcatctgcgt
     6961 cgggcgccac gttgccaaca acaacttgtt catccagttc gcgagcatgg cttgggcatg
     7021 caatatccag gcgaagaagg atgcgcaggg gaacccgatc atgcctgatg cggataagtg
     7081 cacgtcggat ttgacgatgc acaccgtccc attcgattgc gatataacac caaggttccc
     7141 cgaagttccg tccatcattg cccagtccat tgagttgaat aattacggcc aaatttgaag
     7201 tgcttttaac taagaattat tagtcttttc tgcttatttt ttcatcatag tttagaacac
     7261 tttatattaa cgaatagttt atgaatctat ttaggtttaa aaattgatac agttttataa
     7321 gttacttttt caaagactcg tgctgtctat tgcataatgc actggaagga gaaaaaaaag
     7381 gtgcacacgc gtggcttttt cttgaatttg cagtttgaaa aataactaca tggatgataa
     7441 gaaaacatgg agtacagtca ctttgagaac cttcaatcag ctggtaacgt cttcgttaat
     7501 tggatactca aaaaagatgg atagcatgaa tcacaagatg gaaggaaatg cgggccacga
     7561 ccacagtgat atgcatatgg gagatggaga tgatacctcc attgggccga tgaagttagt
     7621 cgacggatag aagcggttgt cccctttccc ggcgagccgg cagtcgggcc gaggttcgga
     7681 taaattttgt attgtgtttt gattctgtca tgagtattac ttatgttctc tttaggtaac
     7741 cccaggttaa tcaatcacag tttcataccg gctagtattc aaattatgac ttttcttctg
     7801 cagtgtcagc cttacgacga ttatctatga gctttgaata tagtttgccg tgattcgtat
     7861 ctttaattgg ataataaaat gcgaaggatc gatgaccctt attattattt ttctacactg
     7921 gctaccgatt taactcatct tcttgaaagt atataagtaa cagtaaaata taccgtactt
     7981 ctgctaatgt tatttgtccc ttatttttct tttcttgtct tatgctatag tacctaagaa
     8041 taacgactat tgttttgaac taaacaaagt agtaaaagca cataaaagaa ttaagaaaat
     8101 gagccacttc tcatggtcca acatctccag cgcagcgccc tcttcctctt catacgctga
     8161 gctctacccc tctctcacgt catggacact cagtgttccg gtcgccatag tgctccttgt
     8221 agcagcgcgt tcctatatgg caaggcctaa ggggaagctt cctcctgggc cgccaggatt
     8281 acccgtcata ggaaatctgc atcaactgac agataagctc tgggtaacgc tcgagaagtg
     8341 gactaaacaa tacggccccg tagtctatct aaatatcgcc ggccaaaaca caatcgtcct
     8401 tggcacgcac aaagccgccg ccgatctcct cgatcgtaga gcgggcatct actctgaccg
     8461 accacgaaat atcgtcgcag gagagctgct gacgggcggt ttggtatttg cgttcgctca
     8521 gcataacgat gtttggaagc ggatgagaag ggcatcgcat gaagcgatga ataaccaagt
     8581 cgccaagtcc tacttcccat tccaagagac tgaatcgtct ctccttgtct ctgagctcct
     8641 cgcttccccg gacctctttg actcccacct ccgccgcgcg tccacgtctc tcgtcttatc
     8701 catgatctac gggctgcctc ccatggaaga cagcagggac cccgtcatct tgcgggtgaa
     8761 tcatttcacg gagagggcgc tgaaggcggc gatcccgggc gcgtatttgg tggagtactt
     8821 cacgtggatg gagcatttgc cgcggtggat gtgtgcctgg agacgatatg ctgaggatgc
     8881 attcaaacgc gattccgtcc tcttcgaaaa cctcttcgct gatgttgtga cccgcctcaa
     8941 aagcggtgat caacgcccca gcgtcgctgc caccatcatc accgaacaag agaagaagaa
     9001 cctcacggat aaagaagctg cctgggtatc tgctacgctc tatgccgctg gtgctgagac
     9061 gacatcgggc caactcgcct ggtttatgga ggcaatggtt ctctaccccg aggtgcaagc
     9121 tcgcgcgcaa gcagagattg atcgcgtcgt cgggcgcgat aggatgccta cattccagga
     9181 ctacgaccat cttccataca tcaaggcaat ggtgaaggag acgctgaggt ggaacggcgt
     9241 cggcagattg ggtgtcccac acaggctggc ccaggacgat tggtacgagg ggcacttcat
     9301 cccgaaggac acgatctgct tagtgaatgt ttggggattg aaccacgata cggaggtcta
     9361 cggtcctgac gtcgatgatt tccgcccgga gaggttcttg aacgaagaag gggagctgaa
     9421 ggccatgcca acagatacca aggacgaagg gcacgtaacc tatggcttcg gaagacggat
     9481 ttgtgttggc cgccatgtct ccaacaacag catgttcatc gagatcgcct ctatcctgtg
     9541 ggcgtgcaat attcgccctg ggaagaacga ggctggagag atcgttatgc cggacccgca
     9601 gaatagtatc gatgaggggc ttgtagtccg tccagcaccc ttcctgtgct ccattaccgc
     9661 taggtcgcct gaagtcgaga ctatcctcgc tgagacgaag gatcgtcatg gtttacctat
     9721 ttagatttaa ctccttaagt tactttaatg atttagtttt tattattaat aattcatgct
     9781 catgacatct catatacacg tttataaaac ttaaatagat tgaaaatgta ttaaagattc
     9841 ctcagggatt cgattttttt ggaagttttt gttttttttt ccttgagatg ctgtagtatt
     9901 tgggaacaat tatacaatcg aaagatatat gcttacattc gaccgtttta gccgtgatca
     9961 ttatcctata gtaacataac ctgaagcata actgacacta ctatcatcaa tacttgtcac
    10021 atgagaactc tgtgaataat taggccactg aaatttgatg cctgaaggac cggcatcacg
    10081 gattttcgat aaagcactta gtatcacact aattggcttt tcgctagtct tatctaaaaa
    10141 ttgcctttat agtccgtctc tccagtcacg gcctgtgtaa ctgattaatc ctgcctttct
    10201 aatcaccatt ctactgttta attaagggat tttgtcttca tcaacggctt ccgcccaaaa
    10261 aaaagtatga cgttttgccc gcaggcgtga agctgcccat cttcacgggc ctgacctcct
    10321 ctgccggaac accggccatc tccaactcat aaattggaga aataagagaa tttcagattt
    10381 tcagaggatg aaaaaaaaaa ggtagagagc ataaaaatgg ggttcacttt ttggcaaagt
    10441 tacagtatgc ttattacata taaatagagt gccgataatg gctttttttc atcttcgaaa
    10501 tacgcttgct actgctcttc cagcgttttt attacttctt tcttgtttct ccttagtata
    10561 taaaatatca agctacaaca agcatacaat caactgtcaa ctgtcaatta tattataata
    10621 cactatgcgc gccgaggctt accttgctct attcgcactg gcgacaaggt cgttcgccac
    10681 cacccagcag ccacttcaga cacaaacgtt tgacgaagga gtgcggacta cagcactaca
    10741 atgttgcaac gcactcgaag acatcctaac ttcctctacc atcctatatc ccgcctcatc
    10801 taaatattcc gcgcagtcat ctacatatta ctccctcgaa caagccgccc aaaagccagc
    10861 gtgtcgcata gcccccgcca caaccgaaga cctctcccga ttaataaaat tcgcgactgc
    10921 cacaaggtgc gagttcgccg tccgcgctgg cggacacatg agctgggaag gctcgtcgaa
    10981 tatcggtcca aacggattta ccatcgacct tgggggcttg aaggaggtgg aactgaagga
    11041 tgatgggaag acggtttcta tcgctcctgg tctgaaatgg tcagaagttt atggattcct
    11101 gaagccacac gggcttcaca cggtgggtgg acgctcctca ggcgttggcg tcggtggttt
    11161 ccttatagga ggtggtgtct ccttcctgtc aacggaaatg ggtctcggaa gtgacaacgt
    11221 tctcgattac gaagtagtcc ttgctgatgg atccatcgtc cacgcaaacg agtcagaacg
    11281 ctccgatctt tactgggcgt tgaaaagcgg cagcaccaac tacgggatcg tcacccgttt
    11341 cttgatgccc acaatacccc gccctcagat atggggtggc tcgctcttct atcacgggag
    11401 tgcgggtccc aggctattca agcaacttgc tacgttcacc gatcgtctca aggaggatcc
    11461 tcacggcatg tccgccatct ccgttgcgtg gagtccagaa atgaaagact acgctatctg
    11521 gtcccccaac gtctacctcg cgccacaagc atacccctcc ccccttttcg atggcctcca
    11581 agatatcgag ccattccaaa gcacgatgcg cgttgcggat ctaggcgaga tcacggacga
    11641 agtagacatg ctctcgccgg gtgggcggaa ttcgcagtgg ttttctgtcg ttttcaagaa
    11701 tgatgcagag ctgccttggg atattcagct gaagggcagg gaaatttttg agccgtacca
    11761 cgatcgtccc ggtgccgcat gggcgatcac cgttcagccc atcaacgaag gcatgattgc
    11821 cgctggacac aagcgagggg gaaacccgtt aggcctctca gtcgaggatg gcgatcaact
    11881 ccttctactt ggctgcgtct tctggaacga tcctcaagat agtgccgtca tgaaggccaa
    11941 aacccaagag tacatcaaag cagcgacgga gttggctcgc gaacgtggac tgctgaacaa
    12001 gtacatctac ttgaactatg cattggatac gcaaaacgtc ctggagagct acggcgcagg
    12061 gaacctgaag aagatgaggg aaatcaagag taaatacgac cccgacaacc ttctggaagt
    12121 atggaagggg ggctggaaat tgtaaggaga ttgataagac ttttctagtt gcatatcttt
    12181 tatatttaaa tcttatctat tagttaattt tttgtaattt atccttatat atagtctggt
    12241 tattctaaaa tatcatttca gtatctaaaa attcccctct tttttcagtt atatcttaac
    12301 aggcgacagt ccaaatgttg atttatccca gtccgattca tcagggttgt gaagcatttt
    12361 gtcaatggtc gaaatcacat cagtaatagt gcctcttact tgcctcatag aatttctttc
    12421 tcttaacgtc accgtttggt cttttatagt ttcgaaatct atggtgatac caaatggtgt
    12481 tcccaattca tcgttacggg cgtatttttt accaattgaa gtattggaat cgtcaatttt
    12541 aaagtatatc tctcttttac gtaaagcctg cgagatcctc ttaagtatag cggggaagcc
    12601 atcgttattc gatattgtcg taacaaatac tttgatcggc gctatgcggc cgccaccgcg
    12661 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgagactcaa
    12721 acaagatata ttttgcccgc cttacagacg aaactagttg gaggttatgg agcatactat
    12781 cacgtgggcg gccactggtg agttactaca ccccaggggc aacgttgatg ctcctaaaaa
    12841 actctggctg gacgcaagcc gtaacacccg tgtcacttca taatcgtttg caattcaggg
    12901 cttgatctac acattaccct gttatcccta ttaattaata cggttatcca cagaatcagg
    12961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    13021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    13081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    13141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    13201 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    13261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    13321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    13381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    13441 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    13501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    13561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    13621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    13681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    13741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    13801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    13861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    13921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    13981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    14041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    14101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    14161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    14221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    14281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    14341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    14401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    14461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    14521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    14581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    14641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    14701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    14761 gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac
    14821 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
    14881 aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt
    14941 aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
    15001 tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt
    15061 tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac
    15121 tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt
    15181 ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg
    15241 actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa
    15301 aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt
    15361 gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct
    15421 ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa
    15481 tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt
    15541 agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg
    15601 atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat
    15661 tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc
    15721 ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg
    15781 aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc
    15841 gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata
    15901 tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc
    15961 gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg
    16021 cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct
    16081 caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag
    16141 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    16201 tcgcgcgttt cggtgatgac ggtgagcgcg tttcggtgat gacggtgaaa acctcggcgc
    16261 gccattaccc tgttatccct aatatacaac gcaaagaagc tggaaaattg gcgagggaat
    16321 cctgtttctg tctatccaag aatgggcatg aggtggcaac cgtcgtgcta gcgtacaggg
    16381 tgcactttgt aaccatttgg gacaccggac actcgctgtt ttcgaaatta ccctttaagc
    16441 gcgggtattg aaccaggctt atgcccaaga tcgtagcaag catttaaatg acggtatcga
    16501 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagat taattaatat
    16561 ctaaaaattg ccttatgatc cgtctctccg gttacagcct gtgtaactga ttaatcctgc
    16621 ctttctaatc accattctaa tgttttaatt aagggatttt gtcttcatta acggctttcg
    16681 ctcataaaaa tgttatgacg ttttgcccgc aggcgggaaa ccatccactt cacgagactg
    16741 atctcctctg ccggaacacc gggcatctcc aacttataag ttggagaaat aagagaattt
    16801 cagattgaga gaatgaaaaa aaaaaaaaaa aaaaaggcag aggagagcat agaaatgggg
    16861 ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc cagtagcgac
    16921 ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc acttcttgtt
    16981 tcttcttggt aaatagaata t
//
