LOCUS       sequence-224277-        5538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter Plasmid. Taurine-inducible expression of GFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOPS0359
FEATURES             Location/Qualifiers
     source          1..5538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1833..2546
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2563..2649
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2742..2836
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2845..2850,2851..2867,2868..2873)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3062..3595
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            3789..3897
                     /note="incP origin of transfer"
     CDS             complement(3908..4570)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      4571..5342
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(5447..5533)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgccgaattc ggatccggag ggctttacta agctgatccg gtggatgacc ttttgaatga
       61 cctttaatag attatattac taattaattg gggaccctag aggtcccctt ttttatttta
      121 aaaatttttt cacaaaacgg tttacaagca taaagcttgc tcaatcaatc accggatcta
      181 gatatcgata ccccgctccg cgatccgcgc cgcagtcagg gccacgccgt cggctatgcg
      241 gttgcgcgcg atcgacgagt agcccatgcg gaagaagcgg catgggccgt cgtccttcgg
      301 gaagaagggc gacccggatt cgatcagcac gccgtccttt cgcaaagcgt tcatcagcaa
      361 gtccgcatcc agaccttccg gcccctcgat ccagaaggac gtgccgccga aagccgcaga
      421 tccggcgatc tccaatccgg catcccggag ggcagcgtcc atgatgatgt ggcggcgatg
      481 atactcctcg cgcatgcggt gcagcacggc atcatagtgt ccgagcgcca ggaaataggc
      541 ggccgttctc tggaggtgcc ccggcggatg ccgcaacatc aaggcccgta gtgcccgcgc
      601 ctcccggatc acggcagccg gcgccaccag atagcccaga cgcaggccgg gaaacaacga
      661 cttggagaaa ctgccgatgt agaacacccg gccgcagcgg tcgaaggcct tgagggccgg
      721 ggaggggggc gccagaaagc tcatctcgaa ctcgtaatcg tcctcgacga ttatgaagtc
      781 cttggaggcg gccgcctcga gcaaggccgc ccgccgttcg atcggcatcg tcgcgccggt
      841 cggcgaatga tggctgggcg tgacgaacac ggcatcgacc tcttctggaa gcgcggcggg
      901 cggcaggccg tccttgtcca cgtcgacgaa ggtgatcccg gcgccgctca agcgaagcga
      961 tgcactcatg tcgggatggc aggggttctc gcagaccgca tgggcgccct gacgcaacag
     1021 gagctggatg acgatccaga gggcgttctg cgcgcccacc gtcaccagga tctcgtcggg
     1081 gttcgcgcgg atgccgcggc ccggcaatgt gcgcgagcag atatagttga caagctggat
     1141 gtcgtccgcc gccgcgaagt cgcccgccat cagctcgaaa tcctcgcgcg ccagcgcccg
     1201 tcgcgcgcag tcgcgccagg ccgtgaggtc gaacagcgac gggtccatct gaccgtatag
     1261 gaacggataa gggtaggttc gccagtcgag gggcttacgc atctgcttgg ccacgatgaa
     1321 ggtcgagcgc aacttggccg accagtcgat ggcatcggcc gcagcatggg cggcctgcat
     1381 cgcgatggct gtcgttgggg gcttgccggc cacacggtag ccgctccggt tcgccgcctc
     1441 cacatagccc tgggaagcga gttcctgata ggcgagcgtt acggtgatcc gcgaaatatt
     1501 cagataggcc gcaagcttgc ggctggaggg caattgggcg cccggcgcca ggcggcccga
     1561 caatacggcc gaaacgatcg tttcgcggat ctgcgcctgc aggcccgttt ggctggcccg
     1621 gtcaaggaaa aagatcgttt cggacacggt cgagcccgcc attgttcacc tcgcccaact
     1681 ggactcatct aacttcctat ctggatataa ggcaatccga agcgtgggcc tatttctatt
     1741 cagcaagcaa agaccgtcat tcaatgtacg gcgcgacccc gcaaaaagcg gggcgacata
     1801 accaaagagg gtaacgacag gaggaagaac aaatgcgtaa aggcgaagag ctgttcactg
     1861 gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc
     1921 gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta
     1981 ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt
     2041 gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg
     2101 aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg
     2161 cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact
     2221 ttaaagaaga tggcaatatc ctgggccata agctggaata caattttaac agccacaatg
     2281 tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca
     2341 acgttgagga tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg
     2401 atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag
     2461 atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca
     2521 cgcatggtat ggatgaactg tacaaatgag cttcccaggc atcaaataaa acgaaaggct
     2581 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2641 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2701 gcaggacgcc cgccataaac tgccagggaa ttccgctctt ggactcctgt tgatagatcc
     2761 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
     2821 ttttattggt gagaatccag gcaattgaca taagcctgtt cggttcgtaa actgtaatgc
     2881 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2941 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagcc tatgcctcgg
     3001 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     3061 gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg
     3121 tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat
     3181 gcgggctgct cttgatcttt tcggtcgtga gttcggcgac gtagccacct actcccaaca
     3241 tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc
     3301 tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaagtttga
     3361 gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggagagc accggaggca
     3421 gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc ttggtgctta
     3481 tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt
     3541 gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaacaatt
     3601 cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattga ctacttttcc
     3661 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     3721 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     3781 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     3841 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
     3901 gccggcccta ccggcgcggc agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt
     3961 ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct
     4021 ccgtttcggt caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg
     4081 gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc
     4141 gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg
     4201 cactgaacac cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga
     4261 ccacgtaggc cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg
     4321 ccaccaagtc cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa
     4381 gagtcttttg gctgaccacc acggcgttct ggtggcccat ctgcgcgacg aggtgatgca
     4441 gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc
     4501 gttccgtttg gacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact
     4561 gcgtggccat gcttatctcc atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa
     4621 tcagctgcaa cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa
     4681 ttacagattt tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt
     4741 gcgtaccccc cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta
     4801 gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct
     4861 ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc
     4921 ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg
     4981 gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc ataggcttgg gtcgttccag
     5041 gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttcctcttg
     5101 gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa
     5161 ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt
     5221 cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg
     5281 tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc
     5341 gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg
     5401 cgaagcccgg agagcaagcc cgtagggggt tactctaggg cggcggattt gtcctactca
     5461 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     5521 ttcgttttat ttgatgcc
//
