LOCUS       sequence-22428-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Smogorzewska A, de Lange T
  TITLE     Different telomere damage signaling pathways in human and mouse 
            cells.
  JOURNAL   EMBO J. 2002 Aug 15. 21(16):4338-48.
  PUBMED    12169636
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Aug 15. 21(16):4338-48."
COMMENT     Sequence Label: pLPC-NFLAG TRF1
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctctctggc tactagagaa ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatcagccac catggactac aaagacgatg
      121 acgataaagg atccgcggag gatgtttcct cagcggcccc gagcccgcgg ggctgtgcgg
      181 atggtaggga tgccgaccct actgaggagc agatggcaga aacagagaga aacgacgagg
      241 agcagttcga atgccaggaa ctgctcgagt gccaggtgca ggtgggggcc cccgaggagg
      301 aggaggagga ggaggaggac gcgggcctgg tggccgaggc cgaggccgtg gctgccggct
      361 ggatgctcga tttcctctgc ctctctcttt gccgagcttt ccgcgacggc cgctccgagg
      421 acttccgcag gacccgcaac agcgcagagg ctattattca tggactatcc agtctaacag
      481 cttgccagtt gagaacgata tacatatgtc agtttttgac aagaattgca gcaggaaaaa
      541 cccttgatgc acagtttgaa aatgatgaac gaattacacc cttggaatca gccctgatga
      601 tttggggttc aattgaaaag gaacatgaca aacttcatga agaaatacag aatttaatta
      661 aaattcaggc tatagctgtt tgtatggaaa atggcaactt taaagaagca gaagaagtct
      721 ttgaaag
//
