LOCUS       sequence-224288-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suitable for experiments in environments where there is no 
            antibiotic selection present. Symbiosis-induced nifH promoter from 
            Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing 
            gusA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOPS0253
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             735..2546
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      2560..2646
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2739..2833
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2932..2951)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2943..3758
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3909..4017
                     /note="incP origin of transfer"
     CDS             complement(4078..5226)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      5619..6150
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(8672..8758)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgccgaattc ggatccggag tcgatgctga ccgcctgatc gccacaatag acgacatccg
       61 atacgattct ccgcttagcg atctcggctt tatcttccac gcgattaacg ttgaacacat
      121 gcttcggcga attgacggaa cttgaaggag atcacccgct atcgcagcgg ggacttcaac
      181 acgcctgcaa ctggtaaccc gtgcatcgcc gcggaacgag gccgatgggt tcgctgccac
      241 tttgaaatgt cgtgcttctg gagcgagact tatatgaaca tttgccatcg ctcgcgccca
      301 gacgccgcgt caatgacgtc ttcctcgtga agtggatcgc tagccgtggc gtagtcgagt
      361 gacatggcgc ttgatcatcg ccggaaacag ccctttgcgg caagtgatca gaattcgtta
      421 ctgctggtct ggtgatggga tacaggctgt tcgaacaatg gtcctagaag ctacacatgg
      481 ataaatgaga catcctgtcg gctggtcaga tattcgacat tgtcatcacc tttgtcggtt
      541 acgtgacaag cgttcctgtt ctcaatcccg catctttgcg cccacataac tggaatcgca
      601 gtaaattatt gttcgtcatc tctatcggcc tgcttggcac gaatcttgtg agctatagcg
      661 aggcagcgga acggcctccg catccacgtt gcgggataac cgcattgcct cgaacacatg
      721 aaggaacgcc aaaaatggtc cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
      781 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
      841 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
      901 ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac
      961 cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     1021 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     1081 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
     1141 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga
     1201 aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc
     1261 tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag
     1321 actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac
     1381 tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag
     1441 tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     1501 ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag
     1561 tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     1621 atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg
     1681 cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag
     1741 agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg
     1801 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     1861 gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga
     1921 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     1981 cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     2041 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     2101 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     2161 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     2221 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     2281 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     2341 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     2401 cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca
     2461 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     2521 aaccgcagca gggaggcaaa caatgagctt cccaggcatc aaataaaacg aaaggctcag
     2581 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     2641 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
     2701 ggacgcccgc cataaactgc cagggaattc cgctcttgga ctcctgttga tagatccagt
     2761 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     2821 tattggtgag aatccaggca attgtgtctc aaaatctctg atgttacatt gcacaagata
     2881 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     2941 ttatgagcca tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg
     3001 atgcggatct gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca
     3061 tttatcgtct gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca
     3121 gcgtggcgaa cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc
     3181 cgctgccgac cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg
     3241 cgattccggg caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca
     3301 ttgtggatgc gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc
     3361 cgtttaacag cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc
     3421 tggtggatgc gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga
     3481 aagaaatgca taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta
     3541 gcctggataa cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg
     3601 tgggcattgc ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta
     3661 gcccgagcct gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca
     3721 aactgcaatt tcatctgatg ctggatgaat ttttctaata attaattgaa ctacttttcc
     3781 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     3841 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     3901 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     3961 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
     4021 gccggccgcg atgcaggtgg ctgctgaacc cccagccgga actgacccca caaggcccta
     4081 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
     4141 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
     4201 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
     4261 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
     4321 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
     4381 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
     4441 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
     4501 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
     4561 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
     4621 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
     4681 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
     4741 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
     4801 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
     4861 caacgcggcc tgcttggctt cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
     4921 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
     4981 ctcctgttcg agccgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
     5041 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacta tcgagccgac
     5101 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
     5161 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
     5221 attcattcac cctccttgcg ggattgcccc ggaattaatt ccccggatcg atccgtcgat
     5281 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     5341 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     5401 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     5461 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     5521 cgtttctgcg gactggcttt ctacgtggct gccatttttg gggtgaggcc gttcgcggcc
     5581 gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga
     5641 agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa
     5701 aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa
     5761 aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt
     5821 caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc
     5881 tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct
     5941 tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc
     6001 tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc
     6061 gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt
     6121 ttccgcgagg tatccacaac gccggcggcc ctacatggct ctgctgtagt gagtgggttg
     6181 cgctccggca gcggtcctga tcccccgcag aaaaaaagga tctcaagaag atcctttgat
     6241 cttttctaca ttactgcatg agcttgtgga agtgtgctga gcataaatga ttctcctagc
     6301 tgttctttgg gtaagtacgc catcaggacg ttgtgagtgg cgcgattttt agcggctgaa
     6361 atcagccctt gagcctgtcg gcaagtcgcg tcatgaggtc catgcgctca tgcaggatcg
     6421 ccacgaccaa cgcgggttcg cccgcacgcg gcaggcaaaa aacgtagtgg tgttcgcagc
     6481 gggccatccg cagcgcggga aagagttcgc tcatgtcctt aaacgggcct tcgccggcgg
     6541 caagcctggc tatgccctgt tccagcttag cgatatagcg gcgcacctgc gccgcgcccc
     6601 actcccggcg cgtgtagcgg atgatgccgc gtagatcggc ttcggcctca gccgtgagga
     6661 tgtaggccgt caagcgcgat ccccgctgag ttcttcatca agaatttcgc cgacgctctt
     6721 ggtggacacc ttgccggcaa gcccatcgtt gatgcggttc cccagcatgg ttttcagttc
     6781 ctgccatgcc tgatcggcat cagcgtcacc ggggaacaga cgttcgaggg cgtattgctt
     6841 aatggtcttg ccctgcaagg cggccagggc tttcaggctc tggtgctgct ggtccgtcat
     6901 gtcgattgtc aggcggctca ttggataacc tccataaaat acacgtaacc acattagcac
     6961 atatgtgggc gtgaggctac agcgcgaggc gcattaaggt cgggaaaatg cgctaggcgc
     7021 atttaaattg cgtattgctg taatgcgcca tgccggctag actaggccca aatgggtata
     7081 cccaatttga ccaaggggga cgcgatgagg gcggccaagc actaccgaca acttctatcc
     7141 atcgacttca acatcgaggc gctggccttc gtgcctggac ccgacggcac acgcggccgg
     7201 cgcatccacg tcctggggcg cgaggtccgc gaccggcccg gcctggtcga gtacctttcg
     7261 ccggcgttcg gctcgcgggt ggcgctggac ggctactgca aggccaattt cgatgcagtg
     7321 ctgcacctgg cgtaccccga tcatcagcaa tggggccacg catgaagcgc cgaagctacg
     7381 ccatgctgcg cgccgctgcc gcgctggccg tcctggtcgt tgcctcgccg gcatgggccg
     7441 agctgcgcgg cgaggtcgtg cgcatcatcg acggcgacac catcgacgtg ctggtagaca
     7501 agcagccggt gcgcgtgcgc ctggtggaca ttgacgcgcc ggaaaagcgg caagccttcg
     7561 gcgaacgtgc gcgccaggcg ctggccggca tggtgttccg ccggcacgtc ctggtcgacg
     7621 agaaggacac cgaccgttac ggccgcacgc tgggcaccgt ctgggtcaac atggagctgg
     7681 ccagccggcc gccgcagccg cgcaacgtca acgccgcgat ggttcaccag ggcatggcgt
     7741 gggcctatcg cttccacggc cgcgcggccg accctgaaat gctgcggctc gaacaggagg
     7801 cgcgaggcaa gcgcgtcggc ctctggtccg atccgcacgc cgtcgagccg tggaaatggc
     7861 gacgcgagag caacaaccgg agggacgaag gttgaaggtc gcccgcatct acctgcgcgc
     7921 cagtacggac gagcagaatc ttgaacgcca ggagagcctt gtagcggcca cgcgggccgc
     7981 cgggtactac gtcgccggca tctaccgcga gaaggcgtcc ggcgcacgcg ccgaccggcc
     8041 cgagctgctg cgcatgatcg cggacctgca acctggtgaa gtcgtcgttg cggagaagat
     8101 cgaccgcatc agccgcttgc cgttggccga ggccgagcgc ctggttgcgt cgatccgggc
     8161 caaaggggcc aagctggccg tgcctggcgt ggtggacctg tcggagctgg ccgccgaggc
     8221 gaacggagtg gcgaaaatcg ttctggaatc cgtccaggac atgcttttga agctcgcctt
     8281 gcagatggcc cgcgacgact acgaggatcg gcgcgagcgt caacgtcagg gtgtccagtt
     8341 ggcgaaggcc gccggccgct acaccggccg caaacgtgac gccggcatgc acgaccgcat
     8401 catcacgctt cgctccggcg gatcgagcat tgccaagacg gccaagctgg tcggatgcag
     8461 cccgagccag gtcaaacgag tgtgggcggc ctggaacgcg cagcagcaaa aataaagccg
     8521 ggcagtgccc ggcttttctc accttttcgc gtcccgcagg gccgctgcga gcgccctacc
     8581 tagatcctcg ctttccccct cggtgtagtc cggccagggc acgaagggcg cggatgcgaa
     8641 cctgttgagc aggtcagatc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     8701 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     8761 gcc
//
