LOCUS       sequence-22429-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Smogorzewska A, de Lange T
  TITLE     Different telomere damage signaling pathways in human and mouse 
            cells.
  JOURNAL   EMBO J. 2002 Aug 15. 21(16):4338-48.
  PUBMED    12169636
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Aug 15. 21(16):4338-48."
COMMENT     Sequence Label: pLPC-NFLAG TRF1 deltaA
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatcagc caccatggac tacaaagacg
      121 atgacgataa aggatccgtg gccgaggccg aggccgtggc tgccggctgg atgctcgatt
      181 tcctctgcct ctctctttgc cgagctttcc gcgacggccg ctccgaggac ttccgcagga
      241 cccgcaacag cgcagaggct attattcatg gactatccag tctaacagct tgccagttga
      301 gaacgatata catatgtcag tttttgacaa gaattgcagc aggaaaaacc cttgatgcac
      361 agtttgaaaa tgatgaacga attacaccct tggaatcagc cctgatgatt tggggttcaa
      421 ttgaaaagga acatgacaaa cttcatgaag aaatacagaa tttaattaaa attcaggcta
      481 tagctgtttg tatggaaaat ggcaacttta aagaagcaga agaagtcttt gaaagaatat
      541 ttggtgatcc aaattctcat atgcctttca aaagcaaatt gcttatgata atctctcaga
      601 aagatacatt tcattccttt tttcaacact tcagctacaa ccacatgatg gagaaaatta
      661 agagttatgt gaattatgtg ctaagtgaaa aatcatcaac ctttctaat
//
