LOCUS       sequence-22430-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Smogorzewska A, de Lange T
  TITLE     Different telomere damage signaling pathways in human and mouse 
            cells.
  JOURNAL   EMBO J. 2002 Aug 15. 21(16):4338-48.
  PUBMED    12169636
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Aug 15. 21(16):4338-48."
COMMENT     Sequence Label: pLPC-NFLAG TRF1 deltaM
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatcagc caccatggac tacaaagacg
      121 atgacgataa aggatccgcg gaggatgttt cctcagcggc cccgagcccg cggggctgtg
      181 cggatggtag ggatgccgac cctactgagg agcagatggc agaaacagag agaaacgacg
      241 aggagcagtt cgaatgccag gaactgctcg agtgccaggt gcaggtgggg gcccccgagg
      301 aggaggagga ggaggaggag gacgcgggcc tggtggccga ggccgaggcc gtggctgccg
      361 gctggatgct cgatttcctc tgcctctctc tttgccgagc tttccgcgac ggccgctccg
      421 aggacttccg caggacccgc aacagcgcag aggctattat tcatggacta tccagtctaa
      481 cagcttgcca gttgagaacg atatacatat gtcagttttt gacaagaatt gcagcaggaa
      541 aaacccttga tgcacagttt gaaaatgatg aacgaattac acccttggaa tcagccctga
      601 tgatttgggg ttcaattgaa aaggaacatg acaaacttca tgaagaaata cagaatttaa
      661 ttaaaatt
//
