LOCUS       sequence-224318-        6837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains an optimized 48-mer TetO repeat for imaging of desired 
            loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Tasan I, Sustackova G, Zhang L, Kim J, Sivaguru M, HamediRad M, Wang
            Y, Genova J, Ma J, Belmont AS, Zhao H
  TITLE     CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live 
            cell imaging of endogenous loci.
  JOURNAL   Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 
            10.1093/nar/gky501.
  PUBMED    29912475
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501."
COMMENT     Sequence Label: pSP2-48-merTetO-EFS-BLaR
FEATURES             Location/Qualifiers
     source          1..6837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..97
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    182..200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    285..303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    330..348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    359..377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    388..406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    433..451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    462..480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    491..509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    536..554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    565..583
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    594..612
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    639..657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    669..687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    698..716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    743..761
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    772..790
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    801..819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    846..864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    875..893
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    904..922
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    948..966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    977..995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1006..1024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1051..1069
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1080..1098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1109..1127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1152..1170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1181..1199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1210..1228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1255..1273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1284..1302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1313..1331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1358..1376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1387..1405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1416..1434
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1461..1479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1490..1508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1519..1537
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1564..1582
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1593..1611
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1622..1640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1657..1690
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1742..1953
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1961..1970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1967..2365
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2694..2828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2841..2874
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2921..3112
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        3171..3382
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3389..3398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3395..4525
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4580..4701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4837..4854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5008..5596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5088..5107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5767..6555)
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     6390..6409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6629..6733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6801..6819
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgccctagg aatgggttag tccctatcag tgatagagag aagggtgttt ccctatcagt
       61 gatagagatg gtggtgattc cctatcagtg atagagaatt gaaatttact aggagaatag
      121 aagtccctat cagtgataga gaatattggt gttccctatc agtgatagag agggaatagt
      181 ttccctatca gtgatagaga tagtttgggg actagggaag agtttgtccc tatcagtgat
      241 agagagtgtg tatagtccct atcagtgata gagaagaagt aatgtcccta tcagtgatag
      301 agaagttaat gtaactagga aaaatataat ccctatcagt gatagagaaa ttttgagatc
      361 cctatcagtg atagagattg ggagtggtcc ctatcagtga tagagatgga agaaggacta
      421 gggaatgtat gatccctatc agtgatagag aagagttagt gtccctatca gtgatagaga
      481 gtagttggat tccctatcag tgatagagat aggggtgata ctaggtgggg gattgtccct
      541 atcagtgata gagaggaaaa aggttcccta tcagtgatag agagttggtg gaatccctat
      601 cagtgataga gagtaaatgt taactaggaa gatgagtttc cctatcagtg atagagattg
      661 tatgaagttc cctatcagtg atagagatta tttaggttcc ctatcagtga tagagaggtg
      721 agttggacta ggtaatagat ggtccctatc agtgatagag aggggtattg gtccctatca
      781 gtgatagaga gtatagttat tccctatcag tgatagagaa gatagtgata ctaggaggag
      841 ttgggtccct atcagtgata gagattagag atggtcccta tcagtgatag agagtaggaa
      901 atttccctat cagtgataga gataaagggt gactaggaag tattgaatcc ctatcagtga
      961 tagagaatat attgtgtccc tatcagtgat agagagtagg gtggttccct atcagtgata
     1021 gagaaggtgg ggatactagg tgggtgatat tccctatcag tgatagagat tatagagtgt
     1081 ccctatcagt gatagagata gggttgattc cctatcagtg atagagaagg tttggaaact
     1141 aggtagaggt atccctatca gtgatagaga ggtgttaggg tccctatcag tgatagagag
     1201 gataaagtgt ccctatcagt gatagagatg ggttaaagac taggtagtgg aaagtcccta
     1261 tcagtgatag agagattttt atttccctat cagtgataga gagaggggat aatccctatc
     1321 agtgatagag attgtagagt tactagggaa gtggttgtcc ctatcagtga tagagagtag
     1381 agggagtccc tatcagtgat agagaaaagg tggtttccct atcagtgata gagaataagt
     1441 tagtactagg gtgtaattta tccctatcag tgatagagag tatagagggt ccctatcagt
     1501 gatagagagt aatatggttc cctatcagtg atagagatgg aggtgggact aggagtttag
     1561 atttccctat cagtgataga gaggtaattt tgtccctatc agtgatagag attagtatgg
     1621 ttccctatca gtgatagaga gggttgtgat actagtataa cttcgtataa tgtatgctat
     1681 acgaagttat catatggcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     1741 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     1801 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     1861 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     1921 ctttttcgca acgggtttgc cgccagaaca caggaagctt gccaccatgg ccaagccttt
     1981 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     2041 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     2101 tgtcaatgta tatcatttta ctgggggacc ttgcgcagaa ctcgtggtgc tgggcactgc
     2161 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     2221 catcttgagc ccctgcggac ggtgccgaca ggttcttctc gatctgcatc ctgggatcaa
     2281 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     2341 ctctggttat gtgtgggagg gctaaccctt atacgcaagg gagtagatgc cgaccgaaca
     2401 agagctgatt tcgagaacgc ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg
     2461 agagaacggc cttacgcttg gtggcacagt tctcgtccac agttcgctaa gctcgctcgg
     2521 ctgggtcgcg ggagggccgg tcgcagtgat tcaggccctt ctggattgtg ttggtcccca
     2581 gggcacgatt gtcatgccca cgcactcggg tgatctgact gatcccgcag attggagatc
     2641 gccgcccgtg cctgccgatt gggtgcagat cagcctcgag gccagctagc ttgaacttgt
     2701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2821 tctggatcac atgtttcgaa ataacttcgt ataatgtatg ctatacgaag ttatctcgag
     2881 agcgctgcta gcttaaggta ccatcgatag atctgggccc gtgaccaaac aggaaaaaac
     2941 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa
     3001 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
     3061 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacttaagcc
     3121 tgagggctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     3181 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
     3241 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     3301 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     3361 cgggtttgcc gccagaacac aggggcgcgc caccatggct tcgtacccct gccatcaaca
     3421 cgcgtctgcg ttcgaccagg ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt
     3481 gcgccctcgc cggcagcaag aagccacgga agtccgcctg gagcagaaaa tgcccacgct
     3541 actgcgggtt tatatagacg gtcctcacgg gatggggaaa accaccacca cgcaactgct
     3601 ggtggccctg ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga cttactggca
     3661 ggtgctgggg gcttccgaga caatcgcgaa catctacacc acacaacacc gcctcgacca
     3721 gggtgagata tcggccgggg acgcggcggt ggtaatgaca agcgcccaga taacaatggg
     3781 catgccttat gccgtgaccg acgccgttct ggctcctcat atcggggggg aggctgggag
     3841 ctcacatgcc ccgcccccgg ccctcaccct catcttcgac cgccatccca tcgccgccct
     3901 cctgtgctac ccggccgcgc gataccttat gggcagcatg accccccagg ccgtgctggc
     3961 gttcgtggcc ctcatcccgc cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc
     4021 ggaggacaga cacatcgacc gcctggccaa acgccagcgc cccggcgagc ggcttgacct
     4081 ggctatgctg gccgcgattc gccgcgttta cgggctgctt gccaatacgg tgcggtatct
     4141 gcagggcggc gggtcgtggc gggaggattg gggacagctt tcggggacgg ccgtgccgcc
     4201 ccagggtgcc gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt
     4261 taccctgttt cgggcccccg agttgctggc ccccaacggc gacctgtaca acgtgtttgc
     4321 ctgggccttg gacgtcttgg ccaaacgcct ccgtcccatg cacgtcttta tcctggatta
     4381 cgaccaatcg cccgccggct gccgggacgc cctgctgcaa cttacctccg ggatggtcca
     4441 gacccacgtc accacccccg gctccatacc gacgatctgc gacctggcgc gcacgtttgc
     4501 ccgggagatg ggggaggcta actgacgatg ataacgcgac tctagagtcg gggcggccgg
     4561 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     4621 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4681 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     4741 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     4801 aaggatccgt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     5461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6061 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6361 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     6421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6541 caccagcgtt tctggagtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6781 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaat
//
