LOCUS       sequence-224333-        9756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BzoCas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pC0057	BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
FEATURES             Location/Qualifiers
     source          1..9756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3723..3741)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3737..3784
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(3959..3964,3965..3981,3982..3987))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4014..4032)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4100..4204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4205..4273,4274..5065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4423..4442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5236..5824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5725..5744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5978..5995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6010..6149
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6075..6094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6235..6257
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6251..6442)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7754..8836)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8798..8817
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8837..8914)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9120..9139
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9223..9242
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9223..9241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9242..9266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9281..9303
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9322..9339
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             9349..9366
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(9379..9402,9403..9438,9439..9462)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             9463..9756
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 tctatggaga acaagacctc cctgggcaac aatatctact ataatccctt caagccccag
       61 gataagagct acttcgccgg ctacttcaac gccgccatgg agaataccga ctccgtgttc
      121 cgggagctgg gcaagagact gaagggcaag gagtacacat ctgagaactt ctttgacgcc
      181 atctttaagg agaatatctc tctggtggag tacgagcggt atgtgaagct gctgagcgat
      241 tatttcccca tggcccggct gctggacaag aaggaggtgc ctatcaagga gagaaaggag
      301 aacttcaaga agaacttcaa gggcatcatc aaggccgtgc gggatctgag aaacttttac
      361 acccacaagg agcacggcga ggtggagatc acagacgaga tcttcggcgt gctggatgag
      421 atgctgaagt ccaccgtgct gacagtgaag aagaagaagg tgaaaaccga caagacaaag
      481 gagatcctga agaagtctat cgagaagcag ctggatatcc tgtgccagaa gaagctggag
      541 tacctgaggg acaccgcccg caagatcgag gagaagcgga gaaaccagag ggagagggga
      601 gagaaggagc tggtggcacc ttttaagtat tctgataagc gggacgacct gatcgccgcc
      661 atctacaatg acgccttcga cgtgtatatc gacaagaaga aggattccct gaaggagagc
      721 tccaaggcca agtacaacac aaagtctgac cctcagcagg aggagggcga tctgaagatc
      781 ccaatcagca agaatggcgt ggtgttcctg ctgtccctgt ttctgaccaa gcaggagatc
      841 cacgccttca agagcaagat cgccggcttt aaggccacag tgatcgacga ggccaccgtg
      901 tccgaggcca cagtgtctca cggcaagaac agcatctgtt tcatggccac ccacgagatc
      961 tttagccacc tggcctacaa gaagctgaag aggaaggtgc gcacagccga gatcaactac
     1021 ggcgaggccg agaatgccga gcagctgtcc gtgtatgcca aggagacact gatgatgcag
     1081 atgctggacg agctgtctaa ggtgcctgat gtggtgtacc agaacctgag cgaggacgtg
     1141 cagaaaacct tcatcgagga ctggaatgag tatctgaagg agaacaatgg cgacgtgggc
     1201 acaatggagg aggagcaggt catccaccca gtgatccgga agagatacga ggataagttc
     1261 aactattttg ccatcaggtt cctggacgag ttcgcccagt ttcctaccct gcgctttcag
     1321 gtgcacctgg gcaactacct gcacgattcc cggccaaagg agaatctgat ctctgacagg
     1381 cgcatcaagg agaagatcac agtgtttggc agactgtccg agctggagca caagaaggcc
     1441 ctgttcatca agaacaccga gacaaatgag gatagggagc actactggga gatcttcccc
     1501 aaccctaatt atgactttcc aaaggagaac atctccgtga atgacaagga ttttcccatc
     1561 gccggctcta tcctggatcg cgagaagcag cctgtggccg gcaagatcgg catcaaggtg
     1621 aagctgctga accagcagta cgtgtctgag gtggacaagg ccgtgaaggc ccaccagctg
     1681 aagcagcgga aggcctctaa gcccagcatc cagaatatca tcgaggagat cgtgcccatc
     1741 aacgagagca atcctaagga ggcaatcgtg ttcggaggac agcctaccgc ctacctgtct
     1801 atgaacgata tccacagcat cctgtatgag ttctttgaca agtgggagaa gaagaaggag
     1861 aagctggaga agaagggcga gaaggagctg agaaaggaga tcggcaagga gctggagaag
     1921 aagatcgtgg gcaagatcca ggcccagatc cagcagatca tcgacaagga taccaacgcc
     1981 aagatcctga agccctacca ggacggcaat agcacagcca tcgataagga gaagctgatc
     2041 aaggacctga agcaggagca gaacatcctg cagaagctga aggatgagca gaccgtgagg
     2101 gagaaggagt acaatgactt catcgcctat caggataaga accgcgagat caataaggtg
     2161 agggatcgca accacaagca gtacctgaag gacaatctga agaggaagta tcctgaggcc
     2221 ccagccagga aggaggtgct gtactatcgc gagaagggca aggtggccgt gtggctggcc
     2281 aacgatatca agcggttcat gcctaccgac tttaagaatg agtggaaggg cgagcagcac
     2341 tccctactgc agaagtctct ggcctactat gagcagtgca aggaggagct gaagaacctg
     2401 ctgccagaga aggtgttcca gcacctgccc tttaagctgg gcggctactt ccagcagaag
     2461 tacctgtatc agttttacac ctgctatctg gacaagaggc tggagtatat cagcggcctg
     2521 gtgcagcagg ccgagaactt caagtccgag aataaggtgt ttaagaaggt ggagaacgag
     2581 tgtttcaagt ttctgaagaa gcagaattac acacacaagg agctggatgc ccgggtgcag
     2641 tccatcctgg gctatccaat cttcctggag agaggcttta tggacgagaa gcccaccatc
     2701 atcaagggca agacattcaa gggcaacgag gccctgttcg ccgattggtt tcgctactat
     2761 aaggagtacc agaacttcca gaccttttac gacacagaga attatccact ggtggagctg
     2821 gagaagaagc aggccgatcg gaagagaaag accaagatct atcagcagaa gaagaatgac
     2881 gtgtttacac tgctgatggc caagcacatc ttcaagagcg tgttcaagca ggacagcatc
     2941 gatcagttct ccctggagga cctgtaccag agccgggagg agagactggg aaaccaggag
     3001 agggcaaggc agaccggcga gaggaacaca aattatatct ggaataagac cgtggacctg
     3061 aagctgtgcg atggcaagat cacagtggag aacgtgaagc tgaagaatgt gggcgatttc
     3121 atcaagtacg agtatgacca gcgggtgcag gcctttctga agtacgagga gaacatcgag
     3181 tggcaggcct tcctgatcaa ggagagcaag gaggaggaga attaccccta tgtggtggag
     3241 agagagatcg agcagtacga gaaggtgcgg agagaggagc tgctgaagga ggtgcacctg
     3301 atcgaggagt atatcctgga gaaggtgaag gataaagaaa tcctgaagaa gggcgacaac
     3361 cagaacttca agtactatat cctgaacggc ctgctgaagc agctgaagaa tgaggatgtg
     3421 gagtcctaca aggtgttcaa cctgaatacc gagccagagg atgtgaacat caatcagctg
     3481 aagcaggagg ccaccgacct ggagcagaag gccttcgtgc tgacatatat ccggaacaag
     3541 tttgcccaca atcagctgcc caagaaggag ttctgggact actgtcagga gaagtatggc
     3601 aagatcgaga aggagaaaac ctacgccgag tatttcgccg aggtgtttaa gaaggagaag
     3661 gaggccctga tcaagtaagg ccgcactcga ggcccgaaag gaagctgagt tggctgctgc
     3721 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     3781 tttgctgaaa ggaggaacta tatccggata tcccgcaaga ggcccggcag taccggcata
     3841 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
     3901 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
     3961 taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
     4021 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4081 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4141 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4201 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4261 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4321 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4381 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4441 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4501 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4561 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4621 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4681 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4741 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4801 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4861 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4921 gtcacgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4981 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5041 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5101 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5341 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5941 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6001 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     6061 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     6121 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     6181 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6241 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     6301 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     6361 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     6421 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     6481 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     6541 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     6601 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     6661 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     6721 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     6781 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     6841 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     6901 gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg
     6961 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     7021 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat
     7081 tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
     7141 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
     7201 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
     7261 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     7321 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     7381 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
     7441 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     7501 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     7561 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     7621 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     7681 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt
     7741 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7801 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
     7861 ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
     7921 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
     7981 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg
     8041 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
     8101 tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa
     8161 aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag
     8221 tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg
     8281 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac
     8341 cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata
     8401 acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat
     8461 agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg
     8521 cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc
     8581 gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa
     8641 cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat
     8701 tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct
     8761 ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata
     8821 acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca
     8881 taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc
     8941 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     9001 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     9061 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     9121 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     9181 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
     9241 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
     9301 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc
     9361 ggcagccata tggctagctg gagccatccg cagtttgaaa aaggtggtgg tagcggtggt
     9421 ggttcaggtg gtagtgcatg gtcacaccct cagtttgaga aaatgtcgga ctcagaagtc
     9481 aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta
     9541 aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga
     9601 aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg
     9661 tacgacggta ttagaatcca agctgatcag acccctgaag atttggacat ggaggataac
     9721 gatattattg aggctcacag agaacagatt ggtgga
//
