LOCUS       sequence-224338-        5954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for HDR. Contains an attP-CC 
            docking site, the visible GMR-white for identifying backbone 
            incorporation flanked by FRT sites for FLP-mediated removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Blanco-Redondo B, Langenhan T
  TITLE     Parallel Genomic Engineering of Two Drosophila Genes Using 
            Orthogonal attB/attP Sites.
  JOURNAL   G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565.
  PUBMED    30065043
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565."
COMMENT     Sequence Label: pHD-mW-attPCC-FRT (pTL717)
FEATURES             Location/Qualifiers
     source          1..5954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2999..3032)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(3334..3353)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3359..3378)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        3420..3511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3512..3580,3581..4372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3730..3749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4494..5082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4983..5002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5236..5253
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5351..5368
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5352..5368
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5904..5937)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tttttgtatt ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt
       61 tttgaggggg caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaaacaaaca
      121 gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata
      181 tgcgagcacc cggaagctca cgatgagaat ggccagaccc acgtagtcca gcggcagatc
      241 ggcggcggag aagttaagcg tctccaggat gaccttgccc gaactggggc acgtggtgtt
      301 cgacgatgtg cagctaattt cgcccggctc cacgtccgcc cattggttaa tcagcagacc
      361 ctcgttggcg taacggaacc atgagaggta cgacaaccat ttgaggtata ctggcaccga
      421 gcccgagttc aagaagaagc cgccaaagag caggaatggt atgataaccg gcggacccac
      481 agacagcgcc atcgaggtcg aggagctggc gcaggatatt agatatccga aggacgttga
      541 cacattggcc accagagtga ccagcgccag gcagttgaag aagtgcagca ctccggcccg
      601 cagtccgatc atcggatagg caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg
      661 caattcggca atcgttttgc ccagaaagta tgtgtcacag cgataaagtc gacttcgggc
      721 ctccctcata aaaactggca gctctgaggt gaacacattt atcgtggcaa agacgttttg
      781 aaaggtcatg ttggtcagga agaggaagat ggctccgttg atattcatca cgcccacttg
      841 cgtgagttgt tggcccaaaa agatgaggcc aatcaagatg gcaaccatcg ttgtctgaat
      901 aagtcgcact tttacgagga gtggttcctt gagcaccgac agccaggatc gccacaggac
      961 cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac ccattctccg gctgctccag
     1021 tggcttctcc aaatttttgg tggccaacaa ctgctccata tcccgggcta ctttgctaat
     1081 ggcaaaattg tcgcatatct tggcgatccg atcacgggac tcgatctccc gtccgggcac
     1141 aacggccaac acctgtacgt aaaagtccgc cggattgtag ttggtaggac actgggcacc
     1201 cacgtaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta ccctgccctc
     1261 ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct gatgaatggt
     1321 caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga cgctgtgggc
     1381 ggtaaatgag tccagtccgg aggtgggctc atcgcagatc agaagcggcg gatcggttag
     1441 tgcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt tcaccctgcc
     1501 gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga tcacctgatc
     1561 cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca tggcctggaa
     1621 aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct ggacataggc
     1681 gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc gcatcccgga
     1741 tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt
     1801 tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca cgccgcaaac
     1861 gttcttgagc aaatgtttcc tgggcgccgg tatgtgtcgc tcgttgcaga atagtccgcg
     1921 tgtccggttg accagctgcc gccatccgga gcccggctga ttgaccgccc caaagatgtc
     1981 catattgtgc caggcatagg tgaggttctc ggctagttgg ccgctccctg aaccggagtc
     2041 ctccggcgga ctgggtggca ggagcgtgcc gtagtttttg gcctgcccga agccctggtt
     2101 aatgcagctc tgcgaagccg ctccgctgtc accattgttc agatgctcgg cagatgggtg
     2161 tttgctgcct ccgcgaatta atagctcctg atcctcttgg cccattgccg ggatttttca
     2221 cactttcccc tgcttaccca cccaaaacca atcacccacc caatcactca aaaaacaaac
     2281 aaaaataaga agcgagagga gttttggcac agcactttgt gtttaattga tggcgtaaac
     2341 cgcttggagc ttcgtcacga aaccgctcgt gccgaattga cgctatcgac ctattcagag
     2401 ttctcttctt gtcttcaata atgacttctt ggttgatttc agtagttgca gtttttagtt
     2461 taattacttg gttgttggtt acttttaatt gattcacttt aacttgcact ttattgcaga
     2521 ttgtttagct tgttcagctg cgcttgtttg tttgctcagc ttacgcttcg cgatgtgttc
     2581 actttgcttg tttgaattga attgacgctc cgtcgacgaa gcgcctctat ttatactccg
     2641 gcgctctttt cgcgaacatt cgaggcgcgc tctctcgaag caacgagaat agcgtgccgt
     2701 ttactgtgcg acagagtgag agagcaatag tacagagagg gagagtcaca aaacgaatag
     2761 agaataacgg ggcatggcgt acctacctcg actgctactc gacttaaagg catttcaagg
     2821 gtttccactg ggctcgactt aaaggcattt caagggtttc cactgggctc gacttaaagg
     2881 catttcaagg gtttccactg ggctcgactt aaaggcattt caagggtttc cactgggctc
     2941 gacttaaagg catttcaagg gtttccactg ggctcgacag tcaggtgcta gtcgtacgga
     3001 agttcctata ctttctagag aataggaact tcctacgccc ccaactgaga gaactcggag
     3061 gttaccccag ttggggcact acgatcgcag gtgtgcatat gtccgcggcc gctagcatgc
     3121 aagaattcca cctgcttcag cgaagggcga caccccataa ttagcccggg cgaaaggccc
     3181 agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg
     3241 ggagtcccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca
     3301 ggtgggacca ccgcgctact gccgccaggc aaacaagggg tgttatgagc catattcagg
     3361 tataaatggg ctcgcgataa tgttcagaat tggttaattg gttgtaacac tgacccctat
     3421 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3481 aatgcttcaa taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct
     3541 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3601 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3661 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3721 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3781 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3841 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3901 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3961 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4021 cataccaaac gacgagcgtg acaccacgat gcctgtagcg atggcaacaa cgttgcgcaa
     4081 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4141 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4201 tgataaatcc ggagccggtg agcgtggttc tcgcggtatc atcgcagcgc tggggccaga
     4261 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4321 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aagcagagca
     4381 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     4441 cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4501 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4561 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4621 gcgcagatac caaatactgt tcttctagtg tagccgtagt tagcccacca cttcaagaac
     4681 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4741 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4801 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4861 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4921 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4981 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5041 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5101 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5161 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5221 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaggcgagag tagggaactg
     5281 ccaggcatca aactaagcag aaggcccctg acggatggcc tttttgcgtt tctacaaact
     5341 ctttctgtgt tgtaaaacga cggccagtct taagctcggg ccccctgggc ggttctgata
     5401 acgagtaatc gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg
     5461 gggagtttag ggaaagagca tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat
     5521 atatgagaat tatttaacct tataaatgag aaaaaagcaa cgcactttaa ataagatacg
     5581 ttgctttttc gattgatgaa cacctataat taaactattc atctattatt tatgattttt
     5641 tgtatataca atatttctag tttgttaaag agaattaaga aaataaatct cgaaaataat
     5701 aaagggaaaa tcagtttttg atatcaaaat tatacatgtc aacgataata caaaatataa
     5761 tacaaactat aagatgttat cagtatttat tatgcattta gaataaattt tgtgtcgccc
     5821 ttgaactcga ttgacggaag agcctcgagc tgcagaaggc ctaggcgcgc cttatgcatg
     5881 gagatcttta ctagtgctct tctgaagttc ctatactttc tagagaatag gaacttcgga
     5941 tcccggtgtc gagt
//
