LOCUS       sequence-224340-        4283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for phiC31-mediated transgenesis using a genomic 
            standard attP-TT site. Contains an attB-TT site, the visible 
            3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Blanco-Redondo B, Langenhan T
  TITLE     Parallel Genomic Engineering of Two Drosophila Genes Using 
            Orthogonal attB/attP Sites.
  JOURNAL   G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565.
  PUBMED    30065043
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565."
COMMENT     Sequence Label: pGE-attBTT-loxP-DsRed (pTL780)
FEATURES             Location/Qualifiers
     source          1..4283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    353..386
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    455..488
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      714..723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             720..1397
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(850..868)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(900..920)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1118..1137
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1312..1335
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1518..1639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1555..1574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1609..1628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(1656..2210,2211..2241)
                     /label=P element 5' end
     rep_origin      complement(2476..3064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2556..2575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3235..4026,4027..4095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3858..3877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4096..4200)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagctag
      241 agttgttggt tggcacacca caaatatact gttgccgagc acaattccgc tcgaattgcc
      301 ggctgtacac ttaagaagtt cctactgcag gtaatgaatt gccgcggtgc gggtgccagg
      361 gcgtgccctt gggctccccg ggcgcgtact ccacgaattc ttgcatgcaa tgcggccgct
      421 agcagatcta ctagtctcga ggcgcgccgg taccataact tcgtataatg tatgctatac
      481 gaagttatcg tacgggatct aattcaatta gagactaatt caattagagc taattcaatt
      541 aggatccaag cttatcgatt tcgaaccctc gaccgccgga gtataaatag aggcgcttcg
      601 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
      661 agcaaataaa caagcgcagc tgaacaagct aaacaatcgg ctcgaagccg gtcgccacca
      721 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
      781 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      841 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      901 tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg
      961 actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1021 acggcggcgt ggtgaccgtg acccaggact cctccctcca ggacggctcc ttcatctaca
     1081 aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacta
     1141 tgggctggga ggcgtccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     1201 tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct
     1261 acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca
     1321 tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcgcc gagggccgcc
     1381 accacctgtt cctgtagggg ccgcgactct agatcataat cagccatacc acatttgtag
     1441 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1501 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1621 aactcatcaa tgtatcttaa gatccaaacc tagtggctgc tgctctaaac gacgcatttc
     1681 gtactccaaa gtacgaattt tttccctcaa gctcttattt tcattaaaca atgaacagga
     1741 cctaacgcac agtcacgtta ttgtttacat aaatgatttt ttttactatt caaacttact
     1801 ctgtttgtgt actcccactg gtatagcctt cttttatctt ttctggttca ggctctatca
     1861 ctttactagg tacggcatct gcgttgagtc gcctcctttt aaatgtctga ccttttgcag
     1921 gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca aatttgggag ttttcaccaa
     1981 ggctgcaccc aaggctctgc tcccacaatt ttctcttaat agcacacttc ggcacgtgaa
     2041 ttaattttac tccagtcaca gctttgcagc aaaatttgca atatttcatt tttttttatt
     2101 ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac
     2161 aagcaaacgt gcactgaatt taagtgtata cttcggtaag cttcggctat cgacgggacc
     2221 accttatgtt atttcatcat gggccagacc cacgtagtcc agcggcagat cggcggcgga
     2281 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     2341 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     2401 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     2461 caagaagaag gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2881 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4201 cacatttccc cgaaaagtgc cacctcgaag tcttagagcc agatatgcga gcacccggaa
     4261 gctcacgatg agaatggcca gac
//
