LOCUS       sequence-224342-        6149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration vector for phiC31-mediated transgenesis using a genomic 
            attP-CC site. Contains an attB-CC site, the visible GMR-white 
            flanked by a FRT site for FLP-mediated removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Blanco-Redondo B, Langenhan T
  TITLE     Parallel Genomic Engineering of Two Drosophila Genes Using 
            Orthogonal attB/attP Sites.
  JOURNAL   G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565.
  PUBMED    30065043
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565."
COMMENT     Sequence Label: pGE-attBCC-FRT-mW (pTL788)
FEATURES             Location/Qualifiers
     source          1..6149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(34..588,589..619)
                     /label=P element 5' end
     rep_origin      complement(854..1442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(934..953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1613..2404,2405..2473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2236..2255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2474..2578)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(2662..2692,2693..2894)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3116..3149
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aaaaaaactc gacaccggga tccaaaccta gtggctgctg ctctaaacga cgcatttcgt
       61 actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat gaacaggacc
      121 taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca aacttactct
      181 gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg ctctatcact
      241 ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt
      301 gcagccttcc actgcgaatc attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg
      361 ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg cacgtgaatt
      421 aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt tttttattcc
      481 acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa
      541 gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg acgggaccac
      601 cttatgttat ttcatcatgg gccagaccca cgtagtccag cggcagatcg gcggcggaga
      661 agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc
      721 agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt
      781 aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca
      841 agaagaaggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      901 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      961 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1021 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1081 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1141 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1201 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1261 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1321 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1381 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2581 catttccccg aaaagtgcca cctcgaagtc ttagagccag atatgcgagc acccggaagc
     2641 tcacgatgag aatggccaga ccatgatgaa ataacataag gtggtcccgt cggcaagaga
     2701 catccactta acgtatgctt gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg
     2761 tattgagtct gagtgagaca gcgatatgat tgttgattaa cccttagcat gtccgtgggg
     2821 tttgaattaa ctcataatat taattagacg aaattatttt taaagtttta tttttaataa
     2881 tttgcgagta cgcaaagcta gagttgttgg ttggcacacc acaaatatac tgttgccgag
     2941 cacaattccg ctcgaattgc cggctgtaca cttaagaagt tcctactgca ggtaatgaat
     3001 tgccgcggtg cgggtgccag ggcgtgcccc cgggctcccc gggcgcgtac tccacgaatt
     3061 cttgcatgca atgcggccgc tagcagatct actagtctcg aggcgcgccg gtaccgaagt
     3121 tcctattctc tagaaagtat aggaacttcc gtacgactag cacctgactg tcgagcccag
     3181 tggaaaccct tgaaatgcct ttaagtcgag cccagtggaa acccttgaaa tgcctttaag
     3241 tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag cccagtggaa acccttgaaa
     3301 tgcctttaag tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag tagcagtcga
     3361 ggtaggtacg ccatgccccg ttattctcta ttcgttttgt gactctccct ctctgtacta
     3421 ttgctctctc actctgtcgc acagtaaacg gcacgctatt ctcgttgctt cgagagagcg
     3481 cgcctcgaat gttcgcgaaa agagcgccgg agtataaata gaggcgcttc gtcgacggag
     3541 cgtcaattca attcaaacaa gcaaagtgaa cacatcgcga agcgtaagct gagcaaacaa
     3601 acaagcgcag ctgaacaagc taaacaatct gcaataaagt gcaagttaaa gtgaatcaat
     3661 taaaagtaac caacaaccaa gtaattaaac taaaaactgc aactactgaa atcaaccaag
     3721 aagtcattat tgaagacaag aagagaactc tgaataggtc gatagcgtca attcggcacg
     3781 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     3841 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggt gggtgattgg
     3901 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     3961 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatggtgac
     4021 agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc
     4081 acgctcctgc cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag
     4141 aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc
     4201 ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg
     4261 gcgcccagga aacatttgct caagaacgtt tgcggcgtgg cctatccggg cgaacttttg
     4321 gccgtgatgg gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga
     4381 tcgccgcagg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg
     4441 gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc
     4501 tccctaacgg ccagggaaca cctgattttc caggccatgg tgcggatgcc acgacatctg
     4561 acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa
     4621 tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg
     4681 aagcgtctgg cattcgcctc cgaggcacta accgatccgc cgcttctgat ctgcgatgag
     4741 cccacctccg gactggactc atttaccgcc cacagcgtcg tccaggtgct gaagaagctg
     4801 tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag
     4861 ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc
     4921 gaagccgtcg acttcttttc ctacgtgggt gcccagtgtc ctaccaacta caatccggcg
     4981 gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg
     5041 atcgccaaga tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg
     5101 ttggccacca aaaatttgga gaagccactg gagcagccgg agaatgggta cacctacaag
     5161 gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag
     5221 gaaccactcc tcgtaaaagt gcgacttatt cagacaacga tggttgccat cttgattggc
     5281 ctcatctttt tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa cggagccatc
     5341 ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtgttcacc
     5401 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
     5461 tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg
     5521 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
     5581 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
     5641 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
     5701 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     5761 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     5821 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     5881 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
     5941 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
     6001 gagtagccga catatatccg aaataactgt ttgttttttt tttttaccat tattaccatc
     6061 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
     6121 caagatatga cctatagaat acaaaaaaa
//
