LOCUS       sequence-224346-        5860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse full-length SynCAM 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T
  TITLE     SynCAMs organize synapses through heterophilic adhesion.
  JOURNAL   J Neurosci. 2007 Nov 14;27(46):12516-30. doi: 
            10.1523/JNEUROSCI.2739-07.2007.
  PUBMED    18003830
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2739-07"; journalName: "J Neurosci"; date: 
            "2007-11-14- 14"; volume: "27"; issue: "46"; pages: "12516-30"
COMMENT     Sequence Label: pCMV5 mSynCAM3
FEATURES             Location/Qualifiers
     source          1..5860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(226..237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1504..2126
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1703..1722)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2150..2170)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2155..2484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2335..2470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2522..2541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2523..2541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2555..2571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2555..2571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2568..2590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2579..2595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2603..2609,2610..2627,2628..2633))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2648..2669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2786..2803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2957..3545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3037..3056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3716..4507,4508..4576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4339..4358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4577..4681)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4708..5163
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4795..4814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5005..5026
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5289..5311
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5303..5320
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5304..5320
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5481..5860
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agaattcgcc cttcgatggg
      241 ggccccttcc gccctgcccc tgctcctgct cctcgcctgc tcctgggcgc ccggcggggc
      301 caatctttcc caggacgata gccagccctg gacatctgat gaaacagttg tggctggtgg
      361 cacagtggtt ctcaagtgtc aagtaaaaga ccatgaagac tcatctctgc agtggtctaa
      421 ccctgctcag cagaccctat acttcgggga gaagagagcc cttcgagata atcggattca
      481 gctggttagc tctactcccc atgagctcag catcagcatc agcaatgtgg cgctggccga
      541 tgagggggag tacacgtgct ccatcttcac tatgcctgtg cgaaccgcca agtcccttgt
      601 cactgtgctc ggaatcccac agaaacccat aatcactggt tataagtcat cattgcggga
      661 aaaggagaca gccactctaa attgtcagtc ttctgggagc aaacctgcag cccagctcac
      721 ctggaggaaa ggtgaccaag aactccacgg ggaccaaaca cgaatccagg aagatcccaa
      781 cgggaaaacc ttcactgtga gcagctcagt gtcattccag gttacccggg aggatgatgg
      841 agcaaacatc gtgtgctctg tgaaccatga atctctgaag ggagccgaca gatccacttc
      901 tcagcgcatt gaagtgttat acacaccaac agccatgatt aggccagaac ctgctcatcc
      961 tcgagaaggc cagaagctgt tgttacattg tgaggggcgt ggcaatccag tcccccagca
     1021 gtacgtgtgg gtaaaggaag gcagtgagcc acccctcaag atgacccaag agagtgctct
     1081 catcttcccc tttttgaata agagtgacag tggcacttat ggctgtacag ccacaagcaa
     1141 catgggcagc tatacagcct acttcaccct caatgtcaac gaccccagtc cagtgccctc
     1201 gtcctccagt acctaccacg ccatcattgg agggattgtg gctttcattg tcttcctgct
     1261 gctcattctg ctcattttcc ttggacacta tttgatccgg cacaaaggaa cctacctgac
     1321 acacgaagcg aagggctccg acgatgctcc agatgcggat acggccatca tcaacgcaga
     1381 aggcgggcag tcaggcgggg atgacaagaa ggaatatttc atctaggggc aagggcgaat
     1441 tcagatctgg taccacgcgt atcgataagc ttgcatgcct gcaggtcgac tctagaggat
     1501 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     1561 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     1621 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     1681 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     1741 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     1801 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     1861 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     1921 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     1981 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     2041 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     2101 tccactcagt agatgcctgt tgaattgggt acgcggccag cttggctgtg gaatgtgtgt
     2161 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2221 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2281 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     2341 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     2401 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     2461 tttggaggcc taggcttttg caaaaagctc ctcgaggaac tgaaaaacca gaaagttaat
     2521 tccctatagt gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     2581 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2641 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2701 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2761 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2821 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2881 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2941 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3001 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3061 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3121 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3181 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3241 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3301 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3361 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3421 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3481 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3541 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3601 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3661 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3721 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3781 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3841 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3901 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3961 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4021 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4081 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4141 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4201 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4261 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4321 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4381 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4441 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4501 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4561 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4621 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4681 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     4741 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4801 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4861 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4921 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4981 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5041 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5101 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5161 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     5221 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     5281 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga tctatacatt
     5341 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     5401 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     5461 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     5521 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5581 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5641 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5701 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     5761 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     5821 ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
