LOCUS       sequence-22436-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Guzy RD, Hoyos B, Robin E, Chen H, Liu L, Mansfield KD, Simon MC, 
            Hammerling U, Schumacker PT
  TITLE     Mitochondrial complex III is required for hypoxia-induced ROS 
            production and cellular oxygen sensing.
  JOURNAL   Cell Metab. 2005 Jun;1(6):401-8. doi: 10.1016/j.cmet.2005.05.001.
  PUBMED    16054089
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2005.05"; journalName: "Cell Metab"; date: 
            "2005-06"; volume: "1"; issue: "6"; pages: "401-8"
COMMENT     Sequence Label: pFRET-HSP33 cys
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(16..18,19..21,22..732)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(61..82)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(322..341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     669..690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
       61 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      121 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      181 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac
      241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      481 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      601 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      721 gagctgtaca agtcagatct cgagctcaag cttcgaattc aagaagaggt gacggtttac
      781 gatccgcagg atgtggagtt caaatgcacc tgctcgcgtg aacgttgcgc cgatgcgctg
      841 aaaacgctgc ctgatgaaga agttgatagc atcctggcgg aagatggcga aattgacatg
      901 cattgtgatt actgcggtaa ccactatctg ttcaatgcga tggatattgc tgaaat
//
