LOCUS       sequence-224382-        5046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses scarlet tagged Borcs7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5046)
  TITLE     Koller Lab plasmids
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pScarlet-Borcs7
FEATURES             Location/Qualifiers
     source          1..5046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      243..831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     732..751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1006..1309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1310..1513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1464..1484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1552..1561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1558..2253
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1691..1709)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1959..1978
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2779..2900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2816..2835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2870..2889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2907..3362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3044..3065)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3256..3275
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3389..3493
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3495..3852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3495..3686
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3518..3538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3703..3838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3887..4681
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3887..4681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3941..3960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4551..4570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4869..4888)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4913..4960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
       61 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
      121 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
      181 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      361 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      421 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      481 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      541 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      601 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      661 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      721 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      781 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      841 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      901 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
      961 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     1021 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     1081 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     1141 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     1201 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1261 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1321 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1381 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1441 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1501 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
     1561 gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc
     1621 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccagg gccttcacca agcaccccgc cgacatcccc
     1801 gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac
     1921 aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca
     1981 atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct gaagggcgac
     2041 attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc
     2101 tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac
     2161 atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc
     2221 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctcg actagttgag
     2281 gtgatggcga ctgggacgcc ggattcgcaa gcccgatttg gtcagtccgt gaaggggctt
     2341 ctcacggaga aggtgaacac ctgtggcacc gatgtaatcg cgctcaccaa gcaggtgctg
     2401 aaaggctcgc ggagctccga gctgctgggg caggcagctc gaaacatggt actacaggaa
     2461 gatgccatct tacactcgga agatagttta aggaagatgg caataataac aacacacctt
     2521 cagtaccagc aagaagctat tcagaagaat gtggaacagt cgcctgacct gcaggaccag
     2581 ttgagtcact tactgaagta gcacatcaga tgctaaggag ggcctgtcct acacaaccat
     2641 caggccgtgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     2701 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2761 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2881 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     2941 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3001 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3061 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3121 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3181 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3241 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3301 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3481 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3841 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3901 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3961 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4021 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4081 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4141 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4201 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4261 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4321 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4381 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4441 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4501 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4561 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4621 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4681 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4741 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4801 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4861 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4921 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4981 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5041 tcttcc
//
