LOCUS       sequence-22445-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Chelur DS, Chalfie M
  TITLE     Targeted cell killing by reconstituted caspases.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8.
  PUBMED    17283333
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 13. 104(7):2283-8."
COMMENT     Sequence Label: pTRE-Tight caspase-3 (p12)::nz [TU#817]
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             423..434
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    575..656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(612..631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(667..684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggtgttga tgatgacatg gcgtgtcata aaatacctgt ggaggccgac ttcttgtatg
       61 catactccac agcacctggt tattattctt ggcgaaattc aaaggatggc tcctggttca
      121 tccagtcgct ttgtgccatg ctgaaacagt atgccgacaa gcttgaattt atgcacattc
      181 ttacccgggt taaccgaaag gtggcaacag aatttgagtc cttttccttt gacgctactt
      241 ttcatgcaaa gaaacagatt ccatgtattg tttccatgct cacaaaagaa ctctattttt
      301 atcacccccg ggattggcca aaggacccaa aggaggaccc ttggagggta ccaggctctg
      361 gctctggcgc tcagcttaag aaagagctgc aggcaaacaa gaaagagctg gctcagctga
      421 agtgggaact gcaggcactg aagaaagaac tggctcagtg atctagagga tcataatcag
      481 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      541 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      601 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcctcg
      661 agcttcctcg ctcactgact cgctgcgctc ggtcgttc
//
