LOCUS       sequence-224458-        6480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  surface-displayed Nb2, Arabinose-inducible; medium-copy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Glass DS, Riedel-Kruse IH
  TITLE     A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming 
            Multicellular Morphologies and Patterns.
  JOURNAL   Cell. 2018 Jul 26;174(3):649-658.e16. doi: 
            10.1016/j.cell.2018.06.041. Epub 2018 Jul 19.
  PUBMED    30033369
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06.041"; journalName: "Cell"; date: 
            "2018-07-26- 26"; volume: "174"; issue: "3"; pages: "649-658.e16"
COMMENT     Sequence Label: pDSG312
FEATURES             Location/Qualifiers
     source          1..6480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3611..3682
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3698..3725
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3732..3752
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3753..3810
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(3968..4783)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3968..4783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4691..4710
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4775..4794
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(5378..5923)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5458..5477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6391..6409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(6413..6456)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    6459..6480
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ttactcatgg ttgttatacc
     1261 cggacccggc acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga
     1321 ttgttttttt atgttaatca gaactcattt gcaaatggtg aaaattattt taaattgggt
     1381 tcggattcaa aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa
     1441 actggtgaaa ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg
     1501 tcgttgaata agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag
     1561 cagatcattt tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt
     1621 tcggcacctc ttgttgctgc gggtggtgtt gctggtcaca cgaataaact gactaaaatg
     1681 tccccggacg tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa
     1741 caggcggcga gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa
     1801 gataccgctc ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa
     1861 cattatggaa cggcagaggt taatctgcaa agtggtaata actttgacgg tagttcactg
     1921 gacttcttat taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg
     1981 cgttacattg actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct
     2041 gcaaacatgt tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt
     2101 ttaggtattg gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc
     2161 cgcatgagcg gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat
     2221 ggcttcgata tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg
     2281 atatatgagc agtattatgg tgataatgtt gctttgttta attctgataa gctgcaatcg
     2341 aatcctggtg cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg
     2401 atcgattacc gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt
     2461 tatcagtttg ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga
     2521 acattatcag gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac
     2581 aagaagcagg atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt
     2641 acgcagaaga ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat
     2701 gatagtgcat tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa
     2761 gactaccagg ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg
     2821 gctcgcgcct atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt
     2881 ctgtcgaatg gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact
     2941 tcggctaaag cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg
     3001 gtagctcagg ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg
     3061 gcaaatagtg ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg
     3121 ccaggacagg tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt
     3181 gcggttatat tttttgatgg tgcgactaga caagggcagc ttgttgagag cgggggggga
     3241 agcgtacaag ccgggggatc gctgcgcttg tcgtgtgcag cttccggtat tgattccagc
     3301 tcatactgta tgggttggtt tcgtcagcgt ccgggaaagg agcgcgaggg tgtcgcacgt
     3361 atcaacgggt tgggcggcgt taaaacagct tacgctgact ctgttaaaga tcgtttcacc
     3421 attagtcgtg ataatgcaga aaatactgtt tatttgcaaa tgaactcctt gaaacccgaa
     3481 gatacagcta tttattattg tgctgccaag tttagtccag gttattgcgg aggatcgtgg
     3541 agcaatttcg gatactgggg tcaaggaacg caagtgaccg tatcttcgta ataatactag
     3601 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     3661 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     3721 ctgcgtttat atactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
     3781 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     3841 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3901 gtaatctcga gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca
     3961 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     4021 tatcgatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     4081 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     4141 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     4201 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     4261 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     4321 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     4381 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     4441 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     4501 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     4561 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     4621 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     4681 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     4741 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     4801 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     4861 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     4921 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     4981 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
     5041 tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag
     5101 gcagacctca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     5161 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     5221 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     5281 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     5341 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     5401 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     5461 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     5521 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     5581 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     5641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     5701 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     5761 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     5821 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     5881 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     5941 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6001 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6061 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6121 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6181 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6241 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6301 tcctgcaact ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa
     6361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     6421 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
//
