LOCUS       sequence-224477-       11255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: PF3D7_1123500-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..11255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3231..3242)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4789..5574
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             5581..5646
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5653..5670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5706..5761
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5760..5779)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7997..8015)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8083..8187
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8188..8256,8257..9048)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9219..9807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9708..9727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9961..9978
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10095..10116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10131..10136,10137..10154,10155..10161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10169..10185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10174..10196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10193..10209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10193..10209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        10480..10859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10860..11063
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11014..11034
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11060..11084
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaac
      421 aacaacaatg ctaacaacaa taacaacaac ttcgccaaca tcaataataa caatgccaat
      481 aacggcatcc acaacgacaa caccaagaac cggcctgccg ccggaagcct gaagaacatg
      541 gaagatctgc tgagctactt caaagagcac acgaacaaca acaacaacaa tgacaaccac
      601 gtcgagctgt tcaagaactt cctgaacacc aacaaagaga gcagcggcag caatatcaat
      661 accctgatga agttcctgaa ctccgccacc aagaatgaca acaccggcaa caaccacctg
      721 gacatcggca agctgctgga caccctgaaa gagttcaaca acgcaaacaa gaacaataac
      781 gtgcccagcg ggaacaaccc caacaaccag gccaccaacc ccgctaacaa caacaacaat
      841 aacaacaacc tgaacaagga caactacgtg cagatctttg gcgacctgct caagaacatg
      901 aacatcccga gcaataacaa caacaataac aataataaca acaacaacaa caataacaat
      961 aacaacaaca ataataacaa tgccgccaac aacaacgccg cctccaacaa caacaacgct
     1021 gctagtaaca acaacaacgc agccaacaat aacaacaaca acgccaaggc cgccaacccc
     1081 atggaacagc tgaccaacct gttcagccac ataaataaca acgaccacca cgacgagaag
     1141 ggcgacggcc accctctgga acacatcatg aatctgttcg gccagggcca caacaacggc
     1201 gacgccaagg gcggcaacaa tcccctcgag cacctgacca gcctgctggg acagggacac
     1261 aatgacgggg atggcaaagg cggaaacaac ccactggaac atctgaagaa gatctttgac
     1321 caggggcaca acgatgggga cggaaagggc ggctctaacc ctcttgagca tctgacctcc
     1381 ctgctgtctc agggccataa tgatggcgac gggaaaggtg gtaacaaccc tctgcagcac
     1441 ctcacatcac tgctgagcca gggacataac aatggtgatg ggaagggcgg atccaatccg
     1501 ctggaacacc tgacttctct gctgtcccaa ggacataacg acggagacgg caagggtgga
     1561 aataaccccg ccgagtacct gaagaagctg ttcggcaacc tgcagggcaa caatgcaaac
     1621 ggcatcaacg gcaagaatat gggcgatctg cagagcatgc tgaacaacct gctgaacagc
     1681 gagaagtaca agaagtcccc ttacaccctg gacaagaccg tgtcctccgg cttcggcagc
     1741 cggtccaaag aatccatgct gctggaatac cagcacaaca aggccgagca agagatcctg
     1801 agagagctgc aagagctgat caacaaaaac aagatcaaga atgtggccga gaacaacgag
     1861 aagctgaagc agtacttctc catgctgaag ggcaagtaca acccctacag ctacgagcgg
     1921 aagatcctga acgacatcat caacgaggac gacatcaaga cgaagtacaa catcgacgac
     1981 aacttcgaga agaacctgat cgtgaacgtg cagaacgaca atatggaata cgacgtcgag
     2041 atgaacaacg aagagaaaga aaagaaaacc gagatgttcg aggacatcaa tgagctggac
     2101 gacaaggacg ccctgtccag cggctccatc aacagcgccc tgatgcggaa gaacaaggcc
     2161 aagaacgaga aactgctgcg gggcctgacc accaatatca acgacgacaa tgataacctg
     2221 cacgagtgca actgcaacaa caaactcaac aactgcgtgc tgtcctacat caactacaac
     2281 aatctcgagt acatgctgca gtccctcgac atcgacgtga agggcctgat ccggaagcac
     2341 atgctgctca aaaagaacaa caacaacaac aataataaca acaataacaa tggcaacaac
     2401 ctgtacttcg agaaaaacga ggccttcctg ctgcccctgc ctcccctgac cacaaagtcc
     2461 aatgtgcaga acaccgccaa ttacgtgctg cgggtgcccc gggtgctgtt cctggccagc
     2521 agaaagagct tcagcagcag cttcgacaga agcctgttcc acatctacaa catcatggac
     2581 acccagctga agtggaatac ctacctgtgg ggctacggct tcaagtacta ccccatcttc
     2641 taccccaaga acctgcacac cctgctgcac agccacgaga agcagatcgg cagcgagccc
     2701 ttcgacctga tcttcgtgca cagctccttc gtgtccaact actacaatta ctacttcttc
     2761 ctgaaaaaca tgcccaagat caccaccctc atcttcatga acgacggctg ggacaacaac
     2821 gtgaagaata tcttcctcaa cctgtttccc cacgtgttct tccagaatca agtgaatatc
     2881 tttgagttca atcccctgaa caatatcgac accgtcgccg agaagaagaa actgttccac
     2941 tccctgtgga acaaagtgac ctccggcacc accgccccta gcagctccat gcacgaccac
     3001 gtgatcacca acaagcacaa gaagcgcaag aagatcgaga caaagaccaa caacgtcgtg
     3061 aacaatgagg acgacgtgct gctgaccggc gaccacagca gctcctctag cagcagccct
     3121 ccctcaaaca ataacaacaa caacaataac aacatcaaca aatacgacga agaggacgat
     3181 gaagagaaca aagccctgtg ggccttcatc cctcacggcg tgaaccactg ctgcagctcc
     3241 cactactata agtcctttat catgaatgac gccagcgaca acaacaataa caacaacaac
     3301 tacaacggcg agggcgagat gctgcaggac gataagcaga atcggaacgt ccagaaggtg
     3361 gtcaacccca atttctacta cgaccgggcc agatccctgg aaaatcagca cacccctgcc
     3421 tacgagctga gctattccct gctggacctg ttcctgcacg aatgtagctt caatgagaag
     3481 gacatcttta acaacgctaa gcgggacatc gacatcctgt acctgtacaa cacggccaac
     3541 aattataacg actacctggt gcagaaaatc atggactaca tctataataa taatatgaac
     3601 aatctgaagg acaagatcca gaaatacgag gtggacctga actactggaa gctgtggggc
     3661 ctgcagacca cccacaagct gattaagaac aagatgatcg actacaccaa catgctccag
     3721 cggagcaaag tgtgcatcat cagcagcaag ttcgccggca tgctcaacaa gatggtcatc
     3781 gatgccctgt tctacggctg cgtggtcatc agcgacaaga cccacaacaa agatatcaac
     3841 aagttcatcg tcaccaccaa ggtgcccttc gagtactacg agatcagcga cctcatctat
     3901 aacaaagaaa acatggcctc cctggccaac gatctgatcg acaagattaa caccgccctc
     3961 gaggaagtga agtccggcaa gcggaaccag atgcggatcg acgtgttcaa gaccggcgcg
     4021 ccgtcgacct ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca
     4081 ctcaaccttg gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct
     4141 tcttcccagt cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct
     4201 actagcaacc ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag
     4261 gtctcccttc agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg
     4321 tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc
     4381 tgtgaggtga tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag
     4441 gaggccaggg tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg
     4501 tggcaatgtc tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta
     4561 tccaaagggt tgaattccgg cggcggctcc ggaggcggta ccggtggcgg cgacctagcc
     4621 ccacagatgc ttcgagaact ccaggagact aatgcggcgc tgcaagacgt gagagagctc
     4681 ttgcgacagc aggtcaagga gatcaccttc ctgaagaata cggtgatgga atgtgacgcc
     4741 tgcggtctag acagaaacct gcctccacta gcacctctgg gcccacaccc agaaacgctg
     4801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4921 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggggccct
     5581 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     5641 gacaagggcc ctcaccacca ccatcaccac taataataag gatcccccga cctcgacctc
     5701 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     5761 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     5821 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     5881 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     5941 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     6001 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     6061 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     6121 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     6181 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     6241 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     6301 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     6361 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     6421 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     6481 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     6541 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     6601 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     6661 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     6721 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     6781 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     6841 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     6901 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     6961 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     7021 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     7081 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     7141 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     7201 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     7261 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     7321 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     7381 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     7441 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     7501 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     7561 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     7621 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     7681 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     7741 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     7801 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     7861 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     7921 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     7981 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8041 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8101 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8161 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8221 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8281 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8341 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8401 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8461 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8521 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8581 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8641 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8701 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     8761 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8821 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8881 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8941 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9001 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9061 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9121 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9361 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9841 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9901 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9961 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10021 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10081 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    10141 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    10201 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
    10261 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
    10321 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
    10381 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
    10441 acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt
    10501 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10561 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10621 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10681 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
    10741 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    10801 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    10861 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
    10921 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
    10981 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
    11041 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcctcact ctcttccgca
    11101 tcgctgtctg cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt
    11161 tccagtactc ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac
    11221 ctgagcgagt ccgcatcgac cggatcggaa aacct
//
