LOCUS       sequence-22448-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Zhang S, Ma C, Chalfie M
  TITLE     Combinatorial marking of cells and organelles with reconstituted 
            fluorescent proteins.
  JOURNAL   Cell. 2004 Oct 1. 119(1):137-44.
  PUBMED    15454087
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Oct 1. 119(1):137-44."
COMMENT     Sequence Label: TU#707
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(169..197)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             682..693
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 catgatcttc tactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg cctgcaggtc
      121 gactctagag gatccatggc tagcaaagga gaagaactct tcactggagt tgtcccaatt
      181 cttgttgaat tagatggtga tgttaacggc cacaagttct ctgtcagtgg agagggtgaa
      241 ggtgatgcaa catacggaaa acttaccctg aagttcatct gcactactgg caaactgcct
      301 gttccatggc caacactagt cactactctg tgctatggtg ttcaatgctt ttcaagatac
      361 ccggatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      421 gaaaggacca tcttcttcaa agatgacggc aactacaaga cacgtgctga agtcaagttt
      481 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgacttcaa ggaagatggc
      541 aacattctgg gacacaaatt ggaatacaac tataactcac acaacgttcc catcatggca
      601 gacaaacaag gtggctctgg ctctggcgct cagcttaaga aagagctgca ggcaaacaag
      661 aaagagctgg ctcagctgaa gtgggaactg caggcactga agaaagaact ggctcagtga
      721 gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag
      781 gggccgctgt catcagagta agtttaaact gagttctact aactaacgag taatatttaa
      841 attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
      901 tacatgctct ttctccctgt gct
//
