LOCUS       sequence-224485-       13931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 13931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CTRP-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..13931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4198..4212
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             7465..8250
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             8257..8322
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8329..8346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8382..8437
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8436..8455)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10673..10691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10759..10863
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10864..10932,10933..11724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11895..12483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12384..12403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12637..12654
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12771..12792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12807..12812,12813..12830,12831..12837)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12845..12861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12850..12872
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12869..12885
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12869..12885
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        13156..13535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13536..13739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13690..13710
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13736..13760
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcccac
      421 tacgccaaga acgaggaaac aaacaagaag gacaccaagc tgaagaagaa gcggaacgtc
      481 aagaccttca acaagaagct gaccaacaag gccttcctgc aggtccagca caccgtggcc
      541 accagaagcg tgccacctcc accctgcctg ggcgacgact gcttctgcca gaactactac
      601 gacctgaccc tgatcctgga cgagagcgcc agcatcggca gcaagaactg gaagaaccac
      661 gtgatccctt tcaccgacaa gatcatcaag gacctgacca tcagcaagaa tgaggtgcac
      721 gtgggcatcc tgctgttcag ctccaagaac cgggactacg tgacctacgg cgacgagctg
      781 agataccaga aggacgagct gctgaagaaa gtcgagaagc tgaaaaagga ctactactgc
      841 ggcggaggca ccaagatcct gggcgccctg aagtacagcc tggaaaatta cgccaagcac
      901 aagaacatcc gctacgacgc ccctaaagtg accatcctgt tcaccgacgg caacgagaac
      961 agcgcctcca acaaacagct gctggaaatg ggcctgacct acagaagaga gcgggtcaag
     1021 ctgctggtgc tgggcgtggc cgctgccgag gataacaagc tgaaactgat cgccggctgc
     1081 gaggaaaaca ccaactgccc ctacagcatg aaggccgagt gggagacaat caacgacatc
     1141 accaagcggc tgactaacaa gatctgccac accgagagcg agatcgagcc cgagcccaag
     1201 cctgagccct ccacccctac acactgtcag ggtgatgatt gcttctgcga ggattactat
     1261 gatctcacac tcatcctgga tgaatcccgg tctatcaccc tggacaagtg gaagaaagac
     1321 gtggtgccct tcgccgagaa ggtgctgaac aacctgaaca tcgacaagga caagatccac
     1381 gtgggaatca tgagattcgc caagtccatg aagaccgaca tcggctacga gcaggaaacc
     1441 cggtacatga agaacgacct gatcaagctc gtgcgcgagc tgaaggataa gtacggctac
     1501 ggcggagcca cccacctggt ggacgccctg cagtactccc tgaaaacctt cacccggcac
     1561 cccaacaaca gagtggatgc tcccaaagtc acaatcctct tcacagatgg caatgaggcc
     1621 agcaagaaag agaaggacat ccgggacgtg ggcctgctgt accggaaaga aaacgtgaag
     1681 ctgatcgtcg tgggcgtgaa cctggccacc gagaaaagcc tgaaactgct ggccggatgc
     1741 accgagaacg aagagtgcct gagagtgatc aagtgcgagt ggaacgatct gaccaatatc
     1801 gccaagattc tgaccgacaa aatctgcaat accggcagcg tggaactgcc caagcccgaa
     1861 gagaaccccg agcccgtgga aaagcctaac cctgaagaga atcccaaccc cgtcgagaaa
     1921 cccaccccag aagaaaaccc aaaccctgtg gaaaaaccta cacctgagga aaaccctaat
     1981 ccagtcgaaa agccagagcc cgagaagaac ccttgcatca acatggaaga ttgctactgc
     2041 aaggacttct acgacattac cctggtgctc gacgagtccg cctccatcag cgatctgatc
     2101 tggcggaacg aagtgatccc cttctccctg gaaatcatca agcggatcaa cattgcctac
     2161 aagaacgtgc acatgggagt gctgctgttc tccgagtaca cccgggacat cgtgcggttc
     2221 tacgataacg ccagatacga gaagggcaca ctgcagacca agatcaatga cctgaagcgg
     2281 gactacagaa gcggcaaaaa gacctacatc atccaggctc tgcagtacgc tctgacctac
     2341 tacagcaagc tgagcaacag aaaagaggcc ccaaaagtca ctatgctgtt taccgatgga
     2401 aacgacgcct acgagtctga gaagggcctg caggatatcg ccctgctcta tcgcaaagag
     2461 aacgtcaaac tcctggtcgt gggagtgtcc accgccagcg agaacaaact gaagatgctc
     2521 gtgggctgcg cccctaacgt cgtgtgcccc ttcgtgatca agacagagtg gggactgctg
     2581 aaaaacgtgg ccgaggtgtt cgtgaagaag atctgcgaca acggcgtggt gctgcctccc
     2641 ggcagcccta gcgagtctac acctggcagc ccctccgaga gcacccctgg ctctccaagc
     2701 gagagcacac ccggaagccc cagcgaaagc accccaggca accctgccga gtccacacca
     2761 ggctccccat ccgaatccac tcccggcaac cccgctgaga gcactccagg cagcccatct
     2821 gaatcaaccc caggatcccc aagcgagtcc accccttgca gcggcaccga gtgcctgtgc
     2881 cacaacacct atgatctgac tctgattatc gatgagagcg cctctatcgg ctactccaac
     2941 tgggagaaag aggtggtccc attcaccatc ggcctggcca gcaatctgga aatctctgag
     3001 aagaaagtga acatggggat tctgctcttc agcgataaga tccgcgagtt catcaaatac
     3061 ggccagaaag agagctacga caagaacaac ctcgtgcggc ggatccacga cctgaagaag
     3121 tactacaaga gcggcggctt cagctacatc gtggaagccc tgaaatatgg cctgtacagc
     3181 tatgccaaga gcaccagctc ccggctgaac gtgcccaaag tgaatatcct gctgacagac
     3241 ggaaacaacg ccgacaccag cgacttcatc ctgaccgagg tgtccagcct gtacaagaaa
     3301 gaaaatgtca aactgctgct gatcggcatc ggcggaccca ccatccacaa gctgaggctg
     3361 ctgggcggct gcgacaagtc cgacggcgac tgcccttacg tggtcaaggc cgaatggaac
     3421 aatctcaagt acaccagcaa cctcatcatt gacaagattt gtcacacaga caagccagtg
     3481 gaaaaccccg gcgacaacag cgccgtgtgc aacagcaagg acgattgcat ctgcgccaac
     3541 tacttcgatc tgacttacat cgaggtgccc accctgaact ccgcctacac atggcggagc
     3601 gactttatgg actgctctaa gaacatcatg aacagcctcg tgatcgataa gaacaaagtg
     3661 cacgtgtccc tgatcatcag cctcgagaaa aagagcgtgc accagggctt cgatgacgtg
     3721 aacagctaca acaaaaacga gctgattaag accctgggca agctcgagaa tagcgccgtc
     3781 ctgaacaagg ccaacatcct ggactccctg gtgtacggca tccagcagag cttcggcaag
     3841 ggcaaccgcg agaacgctcc aaaagtgaca atgctcctga ccaactccaa cagcgacatc
     3901 agcgacgaga aagccctgca ggacatctac ctgaattaca aagagaagtc catcaagctc
     3961 ctcatcatcg ggatcggaat caccaacacc ggcaagctgt tcaacgctgg cggctgcaac
     4021 atgaacggcg acaattgccc tcacgtgtac gccagcaagt ccttctccta catcggcggc
     4081 gtggacacct tcctggaagt gaacaagtgt gacagcagca tcgacaacaa cggcggcgat
     4141 aacggcaacg gcgccgaggg caacgtgaac cccgacaccc ctagctgcaa cgacaacgac
     4201 gacgatgaca agtgcaacaa cgatgacagc aacatcatct gcaccaaggt gctggatatc
     4261 gctgtggtcc tggaccagtc cagcaatatc gctaaggacc agtggaacgt gtacatcaag
     4321 cagttcgtca tcaacaccgt gaaccagaat tacctgagca agtaccggtc ccacatcaca
     4381 atcgtgaaga tgggcaagtc caccaaagag aaatggtccc tgaacaaaaa gatcagctac
     4441 cagaagaaga aaattatcaa agaaatcaac aagctgccca tctcctactc caagaagaag
     4501 gatattgcca agtccctcaa atacgtgcgg accaaggtgt tcaagaagtc cgagacaaac
     4561 cggaagaaac tcatcatcat gctggtcgag ggcaagagca actccaatat gaatgacctg
     4621 cggaaagaag tcggcctgct gaaagtcaac aacatcgatt tcttcgccta cgccatcgac
     4681 aatatcgacg agacagagta taagatcctc ggcgattgcg agggctccgt ggacatggga
     4741 ctgatgggca acagccctcc cagccccagc tacctgccct gcaagaatat cgtgaaagtg
     4801 tcctgggaca ccctgctgag cagcaccgac atccacatga agtacatctg caacggctac
     4861 cccgaggacg ccgagtgcag cgagtgggaa gagtggtccc cttgccccga gacatgcccc
     4921 accatcaata acaacaacaa taacgccgct ggcagcaaca atgccgccag gaccaacatc
     4981 agaacctact tccctcagct gtccaagaga gagcggaagg gcccatacac cctgaagggc
     5041 gaagagtaca tgggcgagaa gtacggcagc agctgcatgg aactgaagtc tatcgagtac
     5101 agaagctgcc ccatcaacgc cggctgtaac gacatgtgcg gcgacttcgg cgagtggtcc
     5161 gagtgctctg ccacctgtgg cgagggcatc agagtgcgga acagagacaa tagcctggac
     5221 aatgacgata agtgcaagct ctttaactcc gccgagatgg aagcctgcaa catccaggaa
     5281 tgcgacgaca ataacaatgt ggacatttgc gaggacattg gggagtggtc tgactggtcc
     5341 tcctgcagca agacctgcgg ctacagcacc cggtcccgga ccttcaccat cctgcccgag
     5401 tatatcggcg agtaccccaa ctgcaagatc ttcgagcgga gcgagacaga agtgtgcgcc
     5461 ttcatccccg cctgctccga cgagaactgc tttgagtggg aggaatggaa cgagtggtct
     5521 agcccctgca gccccagaaa gcgggtgcag aaagcccggg tgctcaaaaa ggacgacgtg
     5581 atcatctcca gcggggataa caacaacaac aacaataaca acgccaagcg gggcatgggc
     5641 cacaagaatt ccgccttcac cagctataac aataagaagt ctgacatctg cgaagaggaa
     5701 gtgcggcact acctggataa ggtggaatac gacgaagagt ccacctgtga aaacaagaac
     5761 ccctgcggag attggagtga ttggagcgag tgcgaccgga cctgcaatgt gggcgtgcgg
     5821 atccggcact tcatcagcca catgttcgac atggtcggag atgaggatga gaaagagtgt
     5881 ctcgagtact ataacaaggt ggaaacccag gactgcctgc atctgcctcc ctgcgacggc
     5941 ggagagtgct ccgactggga aacctgggtg gaatgcaaag aagaggacat gatcggcaac
     6001 aactgccaca agcggaacaa gaagatcctg accagaaagc tggaactcct gaagaacaag
     6061 gacatcaccc ggaccaagaa caccgccgac gtgtgcaatg actacaccct gttccgggaa
     6121 gaggactgcc ctcagagcaa tgacgcctgc atcaatgccc tgtgcaacga atgggaagaa
     6181 tggggcgact gcagcagcac atgcggcgag ggaagcttca agatccggaa gcggaaagag
     6241 cccctggaac tgatccccgc cagccaggac atcaacggca atatcggcct gacatgtgcc
     6301 cagcagaaca tcaaggtcga ggaacgcgag gcctgtatcg tgcccgcctg cgaggacgag
     6361 tctaccaacg gcggaaccga ggtggaaggc tccaccccaa gcagcccaag tgactctaac
     6421 aacaatgacg gcagctccgg cgataatacc ggcgactcca acgataagaa aggcatgggc
     6481 accggcgaaa aggtgtccct ggccgctggc gtgatcggac tggtggctct ggcagctggc
     6541 ggcctgatct acggctacaa taccctgaat ggcggcgagc ctccccacag cagcaatatg
     6601 gaattcgaga acgtggaaaa caacgcaggc accgaggaag aggaaaacga ggacttcgag
     6661 gtggtggatg ccgacgaccc catgtggaac ggcgcgccgt cgacctccat cacggcctat
     6721 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     6781 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     6841 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     6901 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     6961 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     7021 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     7081 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     7141 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     7201 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcggc
     7261 ggctccggag gcggtaccgg tggcggcgac ctagccccac agatgcttcg agaactccag
     7321 gagactaatg cggcgctgca agacgtgaga gagctcttgc gacagcaggt caaggagatc
     7381 accttcctga agaatacggt gatggaatgt gacgcctgcg gtctagacag aaacctgcct
     7441 ccactagcac ctctgggccc acacccagaa acgctggtga aagtaaaaga tgctgaagat
     7501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7621 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8221 gagataggtg cctcactgat taagcattgg ggccctgact acaaagacca tgacggtgat
     8281 tataaagatc atgacatcga ttacaaggat gacgatgaca agggccctca ccaccaccat
     8341 caccactaat aataaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     8401 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     8461 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     8521 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     8581 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     8641 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     8701 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     8761 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     8821 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     8881 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     8941 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     9001 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     9061 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     9121 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     9181 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     9241 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     9301 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     9361 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     9421 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     9481 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     9541 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     9601 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     9661 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     9721 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     9781 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     9841 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     9901 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     9961 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
    10021 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
    10081 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10141 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
    10201 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10261 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
    10321 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
    10381 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
    10441 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    10501 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10561 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
    10621 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
    10681 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    10741 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10801 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10861 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10921 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10981 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    11041 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    11101 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    11161 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    11221 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    11281 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    11341 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    11401 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11461 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11521 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11581 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11641 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11701 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11761 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11821 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11881 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11941 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    12001 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    12061 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    12121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    12181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    12241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    12301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    12361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    12421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12541 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    12601 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    12661 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    12721 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    12781 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    12841 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    12901 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    12961 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
    13021 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
    13081 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
    13141 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
    13201 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    13261 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    13321 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    13381 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
    13441 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
    13501 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
    13561 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
    13621 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
    13681 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
    13741 gtttagtgaa ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
    13801 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
    13861 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
    13921 tcggaaaacc t
//
