LOCUS       sequence-224518-       12440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CCp2-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..12440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5974..6759
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             6766..6831
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6838..6855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6891..6946
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6945..6964)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9182..9200)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9268..9372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9373..9441,9442..10233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10404..10992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10893..10912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11146..11163
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11280..11301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11316..11321,11322..11339,11340..11346)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11354..11370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11359..11381
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11378..11394
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11378..11394
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        11665..12044
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12045..12248
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12199..12219
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12245..12269
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccggc
      421 gacgatatca gcaacacctt cttcaagttc gagttctgcg aggccaccag caccttcagc
      481 agcatcggcg agaacggcct gccccagtac gccgccgaga atgccctgac cagaggcagc
      541 ggctactggt gcagcgaggg caagcacaac gtgaacgacg tggtgtcctg gatcggccac
      601 ctgaagaacg tgcggagcct gaacggcatc atcatccact gggcctacac ccctggcgag
      661 gtgtccatcc tggccagcta cgacggcaac gagccctacg aggaagtggt gccctaccag
      721 ctgctggaaa gcagagtggg caacgtggtg cagaacatca tcttcaacca cgtgatccgg
      781 gccaagagca tcaaagtgaa catgcggcac gccatccacg actacttcgg catcaacttc
      841 gccaacgtgc tgggcagccg ggaccccacc ctgagaatcc agagcggcat gagcagcctg
      901 acccaggacc tgtgcctgca gatcgacgag aagaacgagg tggtgctgga cggctgcatc
      961 accgccatca gctacctgga cggccgggac ctgtggaagc tgaacagcaa gaaccagatc
     1021 tacaacccca tcaacaacct gtgcatcacc ctgaaggaca acctgatcgc caacggcggc
     1081 agactgatcc tggaagattg caacgccgcc ctggaacaca acgacggcag aagctcctgg
     1141 cagctgctgc ccaacaacca gctgaagatc ctgcggaacg gcaacttctg tctgagccag
     1201 gacggccaca agagcggcag catcgacgtg gccttccaca aagagtgcac ctccaccctg
     1261 tccagcgaca acaagaacca cgcccctgac aaggtggtgg acggcctgct ggacaccttc
     1321 tgggtgtccc aggaattcaa cctggacacc gccccagaca gcgtgcactt cgacgtgaac
     1381 ctgggcagca tctacaagct gcagaaggcc atcatcgact ggaagtaccc cgccaccaag
     1441 tacagcatca gcctgagcaa cgatggcgag aactacaaag aggtgtcctc caacctggcc
     1501 aacttcctgc ggagcacaat caacaatctg cacaacaccg aggcccagta catccggctg
     1561 accctgatgg cccctaaccc cgagttcagc gaagagaaca agctgttcta cggcatcaag
     1621 aagttcagcg tctacagcaa ccggatcaag tccatcgtgg acgactgcga caagatcaag
     1681 gacaccgacg acgcccggga caagtacttc ttcgagtttg tgtccgaagt gaacctgcag
     1741 gaaggcaaag agctgaagcg gctggacaac gaactgcagc tgtacgccga gaagatccag
     1801 aacgaggccc tgaagattca gtccctgaac cccaagctga agaagtgcaa gctggaaaaa
     1861 gagaagcggc acaaggacat ctccaacatc aagaacgtga tcctcaagaa catctacgaa
     1921 gtgatcaagc agaccgagaa tatcatcaag atgaaccctc tgagcagcta ctacagcacc
     1981 agcacaaaag agctgggcca gacctccgac aaccccgccg acaactgctt ccacctgaaa
     2041 aatgccctgc cctccagccc cagcggcttc tactacgtgc tgaccacctg tagccagaac
     2101 gtgctgcggg tgttctgcga catgaagatg ggcgccacct actacatccc cagcgtggac
     2161 aacaaaatca tcaacaagct gaaagacgtc gagaacgtgt gtgccaccta cggcctgaac
     2221 cctatccacc tgtaccacga gagccagatc tataccctgc ggaaggtgtt cgacaccatg
     2281 gacatcaata tcgccaaccc cgtgcccctg gccatccgga aagaggacag cgagttctac
     2341 tactccctgg acttccagac caacgtgcac gacatcattg ccaagttcgg cacccctgtg
     2401 ggcaatacct tcgggatcaa caacatcggc atcacattct tcgacagcag cagctccgag
     2461 atgagcgcct tcgtgtgcag cgacaatatc aactccatca acctgcccga gcccttcgtg
     2521 aatctggact gccagtcctc cctgaaagag acaaacgaga tcgagaagat gatcgggaac
     2581 gagtacctga tcaagtgccc tcacgactgc ctggaacggg acatcgagga aagcgtgatc
     2641 ggcggcgagg gaaacatcta cagcgaggac agctccatct gcctgagcgc cattcacgcc
     2701 ggcatctacg acaagcacta cctgatccac ctgagagtga tcaacgccct gaatgagtac
     2761 ggcggcttct tccagaacgg aatcatctcc gagagctttt tcaacaacgc ccaggaagtg
     2821 ggcttcaagc tgtttcacgt gccacccaag tgccccaagg acgacatcac cagcaacatc
     2881 aacaataaca acaattacta ctactatgac aacaacaatg ccaacgccat gtttagcttt
     2941 ctcgagctgg ataacaaaat gaacaacgtc aacgataagt tcgacaacaa cgactacacc
     3001 tacgtggact ccagcaccgc cgacgccatc aacgatctga tcaccatcgt gaacaagcag
     3061 gtcggaagca ccgacaccac ctttctggcc ctgattaaca agcagtccat caagatcatc
     3121 agcaacgcca gaagatacct caagcccacc gagatcttcg agaagaatat cgagctgctg
     3181 agcaatgagg ctctgaagga cgtggaaaag gtgttcaatc tgatcaaggt gctcagctcc
     3241 aagatcaaca gcgaactcga gaaaaagaag tataagctcg agatcctggt ggacgagcgg
     3301 ctgcggcaga aagagttcga gagctggaag ctggacaata tcgacaacat ctatgacacc
     3361 ttcgagatca tcaatagcgt gcagctccag cagatcggca agtggaacat cctggacaac
     3421 cctctgtacg agggcatcaa cggcattacc ctgatccaga atgtgcgggt gtacaacagc
     3481 cccgagaaca gcgtgatcaa tagcttcaac ggcgcctacg ccttcctgag atacaagagc
     3541 ttctacgact tcgtgttcag cacctatgtg aacatcaagg gcgtgggcag cgtgggcctg
     3601 atcttcagaa gctacgataa gtacaacttc tacatgctcg agctgaacaa tgaccggcag
     3661 aagaatgagt tcaacaagcg cctgctgaag tttgagaaca atatcgtgac cgagctggcc
     3721 attgtgaacg gcaatgatct ccaggaaggg gattggttcg tcgtccggat cgagtgcatc
     3781 ggcagcaaga tcattatcac cgtgctcaag accaacaagc ctatctacga gctgcccaag
     3841 cccgatatca tcatcaacga cgacttcacc tccagcggca ccatcggctt ttacacatac
     3901 ggcatcgaca acgtgcagtt caccaacatt gccgtggaat ctgtggaatg cagcaccaaa
     3961 gagatcctga gctacaatat cgctcccatc agctgcaata tctatgaaga gtactacgtg
     4021 ggcaagttta acaaggccta catccctttc gacagcgaga actccaacag cggctccagc
     4081 aactggaagt tcgccaagaa cattggcaac gagaagcacg tcatcctgca gaacagcaat
     4141 atgaagcaga ttgagaacga ggaacagatc cccagcttca tcattctcca gaacaaagcc
     4201 tgccagaccg gcgtgctgaa tttcgccgtg taccccgagt gctccaacgg catcgtgggc
     4261 accatgttca agtttctgga ctccaagaac tacgccatcc tggaaatcgg ctccggcttc
     4321 acccggctga gacagaatgt gaatggcaag tttcagctgc tgtctaagtc catcatcagc
     4381 gggtacaaag agcacatctg gaacagagtg accgtgtcct tctcctccaa caacattaac
     4441 gtcaacctgg gaaccggctt catgacctac cccatcttct ccctgatcgg cctgcacctg
     4501 agcgacggcg agagcgtggg ctttaccagc tacaactgcg ccaatgtggc cttctccaat
     4561 atctacatgc accccttcga cttcaagccc tacacaccca cccctaccct ggataccgag
     4621 tccttcctgc ctcctatctt cagcaagttc gaccaggcca ccatcaaaga agaggaccag
     4681 tcccaggaca tgggctacaa acagatcggc gataacaaga atagcgacat cagcaaggac
     4741 agccctatcg ataagcacag cttcgaggac tccacccggc agatgaagaa ggacgcctac
     4801 tattgcgcca cccacaaaaa catcgtggat atcattaact actgcaacca gtacgacaaa
     4861 gagaacgaca attgcgccaa cgagttctgt accatctgct gcaacaatat tgataccaaa
     4921 gaggaagagg acatccggac ctgcgagatt ctgtgccaga aactggacga caagattctg
     4981 cagaccagcg aagtgctgaa ctacctcaag aagtcctgca tcgagagccc caatgaggaa
     5041 ctgaagaaaa gctgcgagga cgacaatgac aaagaagaat gcctcatcga gatgtgcgaa
     5101 atgtgctgcc agagcgtgac catccccgac gacctgctga ccagccacat ggatatcgac
     5161 tccctgacca accactgcat ctccctgtgc gacaagcccg gcgcgccgtc gacctccatc
     5221 acggcctata agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag
     5281 gaaagcctgc agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg
     5341 atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag
     5401 ttccagctgt ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt
     5461 gctggttctg gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac
     5521 ctggtggtga tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga
     5581 cccacctcac ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc
     5641 aggcaggaga aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg
     5701 agtgaaggtg aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat
     5761 tccggcggcg gctccggagg cggtaccggt ggcggcgacc tagccccaca gatgcttcga
     5821 gaactccagg agactaatgc ggcgctgcaa gacgtgagag agctcttgcg acagcaggtc
     5881 aaggagatca ccttcctgaa gaatacggtg atggaatgtg acgcctgcgg tctagacaga
     5941 aacctgcctc cactagcacc tctgggccca cacccagaaa cgctggtgaa agtaaaagat
     6001 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6061 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6121 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6181 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6241 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6301 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6361 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6421 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6481 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6541 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6601 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6661 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6721 cagatcgctg agataggtgc ctcactgatt aagcattggg gccctgacta caaagaccat
     6781 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggccctcac
     6841 caccaccatc accactaata ataaggatcc cccgacctcg acctctggct aataaaggaa
     6901 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     6961 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     7021 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     7081 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     7141 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     7201 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     7261 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     7321 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     7381 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     7441 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     7501 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     7561 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     7621 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     7681 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     7741 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     7801 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     7861 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     7921 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     7981 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     8041 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     8101 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     8161 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     8221 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     8281 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     8341 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     8401 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     8461 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     8521 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     8581 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     8641 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     8701 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     8761 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     8821 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     8881 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     8941 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     9001 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     9061 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     9121 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     9181 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     9241 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9301 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9361 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9421 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9481 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9541 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9601 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9661 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9721 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9781 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9841 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9901 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9961 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10021 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10081 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10141 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10201 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10261 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10321 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11041 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11101 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11161 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11221 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    11281 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    11341 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    11401 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
    11461 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
    11521 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
    11581 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
    11641 catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
    11701 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    11761 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    11821 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    11881 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
    11941 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
    12001 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    12061 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    12121 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    12181 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    12241 gcagagctcg tttagtgaac cgtcagatcc tcactctctt ccgcatcgct gtctgcgagg
    12301 gccagctgtt gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga
    12361 tcggaaaccc gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca
    12421 tcgaccggat cggaaaacct
//
