LOCUS       sequence-224538-         386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of hnRNPA2 truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Amaya J, Ryan VH, Fawzi NL
  TITLE     The SH3 domain of Fyn kinase interacts with and induces 
            liquid-liquid phase separation of the low-complexity domain of 
            hnRNPA2.
  JOURNAL   J Biol Chem. 2018 Dec 21;293(51):19522-19531. doi: 
            10.1074/jbc.RA118.005120. Epub 2018 Nov 5.
  PUBMED    30397184
REFERENCE   2  (bases 1 to 386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.005120"; journalName: "J Biol Chem"; date: 
            "2018-12-21- 21"; volume: "293"; issue: "51"; pages: "19522-19531"
COMMENT     Sequence Label: hnRNPA2_266-341
FEATURES             Location/Qualifiers
     source          1..386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     gap             46..55
                     /estimated_length=10
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             330..347
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc ntgggctctg ataaannnnn nnnnntcatc
       61 atcacgaaaa tctttatttt caaggtcata tgaaccaggg tggtggttat ggtggtggct
      121 acgataacta tggcggtggt aactatggta gcggcaatta caatgatttt ggcaactaca
      181 accagcaacc gagcaattac ggcccgatga aatccggcaa ttttggcggt tcccgtaata
      241 tgggtggtcc gtatggtggc ggtaactacg gcccgggtgg tagcggtggt tctggcggct
      301 atggtggccg cagccgttac taactcgagc accaccacca ccaccactga gatccggctg
      361 ctaacaaagc ccgaaaggaa gctgag
//
