LOCUS       sequence-224540-        8917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi 
            C, Kirkland TA, Encell LP, Wood KV, Lowik C, Mezzanotte L
  TITLE     Click beetle luciferase mutant and near infrared naphthyl-luciferins
            for improved bioluminescence imaging.
  JOURNAL   Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9.
  PUBMED    29317625
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-9"; volume: "9(1):132. doi"; pages: " 
            10.1038/s41467-017-02542-9"
COMMENT     Sequence Label: ATG-2460
FEATURES             Location/Qualifiers
     source          1..8917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     259..281
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     273..290
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     274..290
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        305..531
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             532..712
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    759..884
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1377..1610
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1795..1839
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2106..2222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2265..2476
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2489..2757
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2814..2823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4458..4511
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4542..5204
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    5295..5883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5348..5368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5766..5777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5957..6190
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6262..6383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6299..6318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6353..6372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6423..6558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6591..6607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6591..6607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6604..6626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6615..6631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6639..6645,6646..6663,6664..6669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6684..6705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6822..6839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6993..7581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7752..8543,8544..8612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8375..8394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8613..8717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8785..8803
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8841..8863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
       61 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
      121 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      181 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      241 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga
      301 cgcgtgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
      361 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      421 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      481 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      721 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      781 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      841 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      901 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      961 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1021 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1081 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1141 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1201 gtaagaccac cgcacagcaa gcggccactg atcttcagac ctggaggagg agatatgagg
     1261 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1321 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1381 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1441 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1501 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1561 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1621 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1681 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1741 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1801 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1861 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1921 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1981 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2041 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     2101 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2161 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     2221 ttatcgatac tagtggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     2281 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     2341 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2401 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     2461 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     2521 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     2581 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     2641 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     2701 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     2761 gctgtgaccg gcgcctactc tagagctagc gaattcgaat ttaaatcgga tccgccacca
     2821 tggttaagag agaaaaaaat gttatttatg gtcctgaacc tctgcatcct ctggaagatc
     2881 tcactgcagg agagatgctg tttcgcgccc tgcgcaagca ctctcacttg ccgcaggctc
     2941 tggtcgacgt ggtgggtgac gagtccctga gctacaagga gttcttcgag gcgactgtcc
     3001 tgctggcaca gagtctccat aactgcggct acaagatgaa cgatgtggtt tccatctgcg
     3061 cagagaacaa cactagattc tttatcccag tgatcgctgc ttggtacatc ggcatgattg
     3121 tcgcaccagt caatgaatct tacattccag atgaactgtg taaggttatg ggaatttcca
     3181 aaccgcagat cgtgttcaca actaagaaca tcctgaacaa ggttctggaa gtgcagagtc
     3241 ggaccaattt catcaagcgt attatcatcc tggacacagt ggagaacatc cacggttgcg
     3301 aatccctccc caacttcatc tctcgttata gcgatggcaa catcgctaat ttcaaacccc
     3361 tgcatttcga tcctgttgaa caggttgccg ccatcctgtg ctcctctggg accaccggac
     3421 tgccgaaagg cgtgatgcag acccatcaga acatctgcgt gaggctgatc cacgccctgg
     3481 acccacgcta tggaactcag ctgatcccag gagtcactgt gctggtttac ctcccttttt
     3541 ttcacgcctt cggtttccac attaccctcg ggtactttat ggtcggcctc cgcgttatca
     3601 tgttccgtcg ctttgaccag gaggctttcc tgaaggccat ccaggattat gaggtgagga
     3661 gtgtgattaa cgtcccaagc gtgatcctgt tcctgtctaa gtcccctctg gttgacaagt
     3721 acgatctgag ctccctgcgt gagctgtgct gcggggctgc accactggcc aaagaagtcg
     3781 cggaagtggc tgccaaacgc ctgaacctgc cgggtatctc ctgtggcttc ggcctgacag
     3841 agagcacatc cgctatcatc cagacactgc gggatgagtt taagtccggt tccctgggcc
     3901 gggttacacc cctgatggca gccaagatcg ccgaccgcga gactgggaag gccctcggac
     3961 ctaaccaggt cggagagctg tgtattaagg gccccatggt gtccaaggga tatgtgaaca
     4021 acgtggaagc tacaaaggag gcgattgatg atgacggatg gctccatagc ggagactttg
     4081 ggtactatga cgaggacgag cacttctatg tggtcgacag gtacaaggag ctgatcaagt
     4141 acaagggctc ccaggtggcc ccagctgaac tggaggagat tctgttgaag aacccttgta
     4201 tcagagatgt ggctgttgtg gggattcctg acctcgaagc aggtgagctg ccctccgctt
     4261 tcgtggtgaa gcagcctggt actgagatca ccgccaaaga ggtgtacgat tacctggctg
     4321 agcgcgtgtc ccacaccaaa tacctccgcg gcggggtccg tttcgtggac tccattccca
     4381 ggaacgtgac cggcaagatc acccgcaagg agctgctgaa gcagctgctg gttaaggctg
     4441 gcggcgttgc ggccgctgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     4501 atcccggccc ttccggaatg gagagcgacg agagcggcct gcccgccatg gagatcgagt
     4561 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca
     4621 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca
     4681 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca
     4741 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca
     4801 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg
     4861 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct
     4921 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata
     4981 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct
     5041 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg
     5101 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca
     5161 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt
     5221 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgctagagct
     5281 gaatctaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5341 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5401 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5461 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5521 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5581 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5641 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5701 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5761 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5821 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5881 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5941 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt
     6001 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6061 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6121 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6181 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6241 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6361 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6421 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6481 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6541 gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat gtcatagctg
     6601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6661 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6721 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6781 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6841 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6901 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6961 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7021 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7081 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7141 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7201 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7261 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7321 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7381 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7441 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7501 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7621 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7681 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7741 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7801 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7861 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7921 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7981 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8041 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8101 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8161 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8221 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8281 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8341 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8401 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8461 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8521 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8581 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8701 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8761 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     8821 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8881 ctgtaagcgg atgccgggag cagacaagcc cgtcagg
//
