LOCUS       sequence-224542-       12461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CCp1-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..12461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5995..6780
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             6787..6852
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6859..6876
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6912..6967
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6966..6985)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9203..9221)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9289..9393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9394..9462,9463..10254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10425..11013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10914..10933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11167..11184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11301..11322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11337..11342,11343..11360,11361..11367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11375..11391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11380..11402
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11399..11415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11399..11415
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        11686..12065
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12066..12269
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12220..12240
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12266..12290
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcccag
      421 gaaagcgcca ccaacttcta caagttcatc gacagcttcg ccagcagcac ctacatgagc
      481 gaggaaagcg gcagcagcgc ctacgacgcc aagcgggcca tccagaacaa ccccaactac
      541 tggtgcagct ccggcaacca cgccaacgac gaggaaatca cctggaccgg ctacctgaac
      601 accaagggct tcatcaaggg cgtgaaggtg tcctgggcct acagccccga gttcgtgaag
      661 atcagcgtgt ccagcgacgg cgagaagtac cggaccatca tcccctacaa gaagatctcc
      721 agcaacgagg ccagcttcga cgagatctac ttcttcaagc ggctggaaga ggccatgagc
      781 atcaagatcg gcctgaagaa cgcccggcac aagtacttcg gcatccggga agtgaagctg
      841 atcggcggag gcaaccccta cttcctgctg ctgagcggca tcagctccga ggaagagatg
      901 tgcctgcagg tcgaggaagg cctgatcaac aacgacaaca ccgccatcat cctggacagc
      961 tgtaccaacg ccctggcctc cggcgacggc agagagctgt ggaaaacaaa cagcaacaac
     1021 caggtcatca gcgccttcag cgaccctccc aagtgcctga gcgtggtcaa cctggacgac
     1081 ctcgagaaca acaagatcgt gctgtacgac tgcctgcggg ccctggaaga tggcgacgga
     1141 aagagcaact ggatcttcga gagcaacagc cagatccggc tgcagaagtc tggcgacgcc
     1201 ttctgcatca gccagaagaa catctacggc aacatccccg gcatccacga catcctgctg
     1261 aacctggatg tgtccatcta cagcaactcc gccctggacg atgaccacaa ccccgacaat
     1321 accatcgacg gcaacctgaa ctcctactgg gccagcgcca ccttcaccga caactacgac
     1381 cacctggtgt acctggtgct ggacctgaac aagatcaccg acctgagccg gatcaagatc
     1441 tactgggagt accctcccct gcactacaat atcgccgtgt ccaccgacaa tcagaacttc
     1501 gccgtggtgt ccgagaacct ggccaacccc gcctacatca ccgtggactc cctgaagaat
     1561 atggaaaccc ggtatatcaa gatcagcatg atcaagaccc accccaagca cggcgagctg
     1621 ggcgacaact tcctgtacgg catccgcagc atcgaggtgc aggccaacaa cctggaaacc
     1681 gtgatcaacc actgccggga cgccgccaac agcgacgacg ccagagacaa gtatttcgtc
     1741 gagtacatca cagagttcga caaggacctg accaacaagc tgatcaacct cgaggacgac
     1801 gtgaccaaga acgtggccag catcagcgac aatctggcca agctggaaga actgctgccc
     1861 aacatcgaga catgcctgga agagaagaaa acctacgatg aggaactgaa agagagcaaa
     1921 gagaaggcca acgacctcaa caacaaactg agcctgctga ccagcgtgaa cgtgaacacc
     1981 ctggactccg atatcctgaa gctgggcatc ctgcccggcg acagctacaa cttccccgcc
     2041 aacgattgcg ccgtgattaa gaacgtgcag gaaaaccctc tgagcggctt ctactggatc
     2101 aagcccaagt gcagccctga gcccctgcgg gtgtactgcg acatggacag ctccaccagc
     2161 atctacatct ggaacggcaa cccacccaag agccccgatc acctgatcac caacatgatc
     2221 aactccgtga acgacatccg gcagcactgc gccgaagtgg gcctgcagcc tctgatcctg
     2281 agaagcaaga accagctgaa cagcctgatc atctctctga agaagatcgg ctacagcctg
     2341 aacggcaaag tgaacatccc tctggcctac gactacagct gcgaccacgg cagctgcagc
     2401 ggcagattcc acgacctgct gaatggcaac atcgacatca gcaccctgat ctacctgaag
     2461 gccagcgaga gccctgacag caccaaagtg cggcagaccg ccggcatctc ctacgacgac
     2521 ggctccttca agttcttcaa tctggaaacc agcgacatct ccgccatcgt gtgcagcacc
     2581 aacagcgccg agaacgacgc cgccctgcag tacctgtcca tcaactgcga gacaaccggc
     2641 atggaagata gcttccacag catcgtgaat accaatatcg tggtgctgtg ccctctgggc
     2701 tgcgacgatg agaagtatca cgacgcctcc atctatggca gccggggcac ctacagcgat
     2761 aacagcgcca tctgcagagc cgccatccac tccgacatca tcgacaacaa gggcggcctg
     2821 gtcaatgtgg ccatcgagag cggcatggac cactacgtgg gcagcattaa caacaatatc
     2881 gagagcatct ccctcaacaa gaacgagaag ggcctgctgg acatcatccc tgaggaaaaa
     2941 gagggcacca acaacatcag agaggaaagc agcatcttcc accacaagac catccgggtg
     3001 tcctccctga tcgaggactg tcccctggac ctgttcctgt tcaaccaggc tagcttcctg
     3061 gaaaagggaa acaacatccg gaacaacaaa ggcaccgagc tgaagtacaa cgacgacgag
     3121 aacatggccg tgaagaattt ccacgagctg atctccaacc tgatggaaaa catcgatgcc
     3181 atccacggcg tggacagcag cgtgatcagc attgtgcagg aagagacaat ccggatcatc
     3241 gaaaagacca agaaagagct gaagcccgcc gacatgctga gcaagaagca gattgaggac
     3301 gccatgaacc tgtacaacct ggccgagaat ctggctatct acctgtacga tctgagcagc
     3361 aagtacatcc aggatctcga gaagctgaag aacaccctcg aggaactcaa gggcgcccag
     3421 aaggtggccc acaacttcgg caccttcaag ctgaactacg agacaatgaa tttcgccacc
     3481 cacttcagcc tgttcgacag caacctcatc aagaacaaag aaagcgtgtg gggctacagc
     3541 gacaccaaca tcctgggcca cgagaacagc atcggccaga tgaactccgt gtctagccag
     3601 gaaatcggcg agggctacta tgccaagctg aagggactga acttctacga cttcgacttc
     3661 aacattgccg tgctgagcag aggcaccggc tgtctgggcg tggtgttccg ggccaaggac
     3721 gacttcaatt tctacctgtt tgacatctgc gacaaggatg gcaccaagag actgagcaag
     3781 gtggaaaacg gccaggtgca catcctgaag aaggtggtca actctgacgt gaccctgaac
     3841 aaccagtgga acaagtacaa gatcatcacc aagcacgcca atatcgacat ctacgaggtg
     3901 gacaaggaca acaacatgat taagatcctg agcagcctgg acgagcggtt cctgtccggc
     3961 accgtgggcc tgtactctca gatctacggc ctgggcacct tctttgacga cctggaagtg
     4021 atcgccctgc cctgcaccca gctgagcgag ctgaacacac tgaacaaaaa cgtgaagtcc
     4081 aactgcccct actacaaaga gaactatctc aacaacctca tgagctacga tatcatctac
     4141 aaccctaaca actacttcaa ctggaacgtc gagaaagaga acgagcagaa ctacctgctg
     4201 tgcagcaaga acgaagagga agtgaaaaac gccaaggatg agaaggatat ctacaccatc
     4261 gtgctgctga agctgcgcga gtgcaccgac ggcaccttta acttcgatat tcaggtgtcc
     4321 gacgacgaaa ccgggaatat cgccaagaag ctgagctaca tctatatcct gttccactac
     4381 aaggacgaaa acaacttcaa cgccctcgag atgaaggacg gcaagctggc tttcctgaca
     4441 aacaagaatg gcaagagctt catcctgtcc gagcggaacg aggaagaaaa cgataacaac
     4501 aaaaacattg agaagcggtt caccttcgtg cagaacgaat ggatccacgt gaacctgcac
     4561 ttcgacaagt ccaccttcaa agtgatcatc atcacaaaca acaacgagga caagttcgtc
     4621 ctgagcgcca agagccggaa cgacgtgccc ctgggcaaag tgggcttcct ggtgcacaat
     4681 ttcgacgaag tgaagttcga ctccatcctg ctcaacagcc ccaccatcac aaaggtggac
     4741 gagaactttc tgcaggtcaa aagcaagacc tgggccaatt gcgaggacag cgtgcacgtg
     4801 ctgcaccggc ggttcagctg cgagactgac atctacccca atgaggccaa agaaaagcac
     4861 atcaagtgca tcaagaattt ctgcaaagag tgctgcctgt accacacaca gctgctggac
     4921 tccaacgaga agaacgagtg cgagaagcac tgcaagcaga acgataatct ggccgccaag
     4981 atgcagaccc tgttcgagaa gttcatcaat cgctgcgtgt ccctgaacga gaatgaggac
     5041 tacgaaacct gcgataagaa cgacaagaaa tgcaaaaaca aagtgtgcgt gctgtgctgc
     5101 aaaaagcacg accccaccac cagcaaagaa ctgaaggtgc tgcccatgaa ccagttcaag
     5161 aagattcagg aaaacgagat catcgagtgc cagctgcagt gcaatatgat ccactccatc
     5221 ggcgcgccgt cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc
     5281 ttcccactca accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag
     5341 gctccttctt cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag
     5401 aagtctacta gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata
     5461 ccccaggtct cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg
     5521 atactgtatc aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact
     5581 ttgacctgtg aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag
     5641 aatcaggagg ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca
     5701 ggggtgtggc aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag
     5761 gttttatcca aagggttgaa ttccggcggc ggctccggag gcggtaccgg tggcggcgac
     5821 ctagccccac agatgcttcg agaactccag gagactaatg cggcgctgca agacgtgaga
     5881 gagctcttgc gacagcaggt caaggagatc accttcctga agaatacggt gatggaatgt
     5941 gacgcctgcg gtctagacag aaacctgcct ccactagcac ctctgggccc acacccagaa
     6001 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6061 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6121 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6181 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6241 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6301 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6361 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6421 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6481 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6541 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6601 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6661 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6721 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6781 ggccctgact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     6841 gacgatgaca agggccctca ccaccaccat caccactaat aataaggatc ccccgacctc
     6901 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     6961 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     7021 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     7081 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     7141 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     7201 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     7261 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     7321 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     7381 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     7441 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     7501 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     7561 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     7621 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     7681 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     7741 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     7801 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     7861 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     7921 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     7981 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     8041 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     8101 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     8161 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     8221 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     8281 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     8341 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     8401 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     8461 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     8521 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     8581 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     8641 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     8701 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     8761 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     8821 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     8881 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     8941 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     9001 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     9061 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     9121 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     9181 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9241 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9601 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9901 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     9961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11221 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    11281 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    11341 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    11401 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
    11461 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
    11521 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
    11581 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
    11641 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga
    11701 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
    11761 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
    11821 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
    11881 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
    11941 caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
    12001 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
    12061 ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg
    12121 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
    12181 gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg
    12241 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc ctcactctct
    12301 tccgcatcgc tgtctgcgag ggccagctgt tgggctcgcg gttgaggaca aactcttcgc
    12361 ggtctttcca gtactcttgg atcggaaacc cgtcggcctc cgaacggtac tccgccaccg
    12421 agggacctga gcgagtccgc atcgaccgga tcggaaaacc t
//
