LOCUS       sequence-224546-        9923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: AAP-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..9923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3457..4242
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4249..4314
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4321..4338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4374..4429
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4428..4447)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6665..6683)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6751..6855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6856..6924,6925..7716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7887..8475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8376..8395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8629..8646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8763..8784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8799..8804,8805..8822,8823..8829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8837..8853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8842..8864
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8861..8877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8861..8877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9148..9527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9528..9731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9682..9702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9728..9752
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaag
      421 ggcaagagac agaacctggt gtccgcctac ctgaaccact tcaagaagtc ctacttcagc
      481 ggcgtgacca gcggcagcga ctgcgtgaac aaggccgagg tgtccagcga caacaacaat
      541 aacaataata acaacaacaa caagatcgcc cacaacttct tcagcaagaa gtaccagcgg
      601 aacttcgaga acaacaacct ggccgagaac caggaaaaca acaaaaacat catctacagc
      661 ggctccaaca tcttcaagaa catctacaac accgagatga tgtcaaacaa caacgccgtg
      721 gacgtgaaca tgatggacaa caaccctgcc gccagactgg aagaactgcg gaccatcatg
      781 aagaagaaca agattgacgt ctacatcctg atcaacagcg acgagcacaa cagcgagatc
      841 atcaacgaga aggacaagaa gatcgtcaag atcaccaact acgctggcgc cgacggaatc
      901 ctgatcgtga ccaaggacaa gcccatcctg tacgtgaacg ccctgtacga gctgcaggcc
      961 atgaacgagc tggaccagaa cctgttcacc ctgcggatca gccggatcga caaccgggac
     1021 gagatcttcg agacaatcag cagcctggaa ttcaacacaa tcgccttcga cggcaagaac
     1081 accgccgtgg tgttctacga gaagctgcgg aaggccctgc tgaacgccta ccccaagaaa
     1141 aagatcgtcg agaagatcat ctataacaac aatttcgacg acgtgaacaa gaaggacgac
     1201 gagaacgtcc tgaacttcct ggtgctggaa aagtccctgg tggaaatcaa ggactaccct
     1261 gtgaacaaca aggccctgta catccacgac cggaagtaca acggcgcctg tgccggcgag
     1321 aagattgaca agctgaagca gagcctgatg tacgacatca agaacgtgga caacctgctg
     1381 ctgagcgagc tggatgagat cgcctatctg ctgaacctgc ggggctacga ctaccagtac
     1441 agccctctgt tctacagcta cctgctgttc cagttcgaca gagaggaaca ggacttctcc
     1501 aagatcgtgt tcttcaccac cgtgaagaac ctgcccgccg atgtgaagaa tctgctcgag
     1561 atcaacaaag tgatcgtgaa agagtacgag gaaatcgtcc cctacctgcg ggacgtggtc
     1621 atccccagca tccccaagca caacgacgac aaccccgact ttaagaagta cgatatcagc
     1681 ctgagcccct acatcaacct gatgatctac aagctgttcg atcggaagaa cgtgctgctg
     1741 cagaacagcc ccgtggtcaa gatgaaggcc gtgaaaaacg acgtggaaat cgacaacatg
     1801 aagcaggccc acatcctgga cggcctggct ctgctgcagt tctttcattg gtgcgagcag
     1861 aagcgcaaga ccaaagagct gttcaacgag gccgagatga gcctgcggca caaggtggac
     1921 tacttcagaa gcaccaagaa gaacttcatc ttccccagct tcagcaccat cagcgccagc
     1981 ggccccaacg ccgccgtgat ccactacgag tgcaccgaca agaccaatgc cgccatcaag
     2041 cccgccatct acctgctcga ctctggcggc cagtacctgc acggcaccac cgacgtgacc
     2101 cggaccaccc actttggcga gcccaccgcc gaggaaaagc ggatctacac cctggtcctg
     2161 aagggccacc tgagactgcg gaaagtgatc ttcgccagct acaccaactc tgccgccctg
     2221 gactttatcg ccagagagaa tctgttcaac aacttcatgg actacaacca cggcaccggc
     2281 cacggcgtgg gcctgaccct gaatgtgcac gagggcggct gcagcatcgg ccctgtggga
     2341 ggcgcccctc tgaagaaaaa catggtgctg tccaacgagc ccggctacta catgaaggat
     2401 aagttcggcg tgcggatcga gaacatgcag tacgtgatca gcaaagagat caccgacacc
     2461 accgagtacc tgagcttcga cgacctgacc atgtaccctt acgagaagaa gctgctggac
     2521 ttcagcctgc tgaccaatca ggaaatcaaa gaactgaacg agtaccacac caccatccgg
     2581 aacaccctgc tgcccctcgt gaagcagtcc cctcaggaat acggcgagtc tgtcgagaag
     2641 tacctgatcg agatcacaga gcctatcgcc atccacaaca acggcgcgcc gtcgacctcc
     2701 atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga
     2761 gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc
     2821 tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc
     2881 aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag
     2941 tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg
     3001 aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg
     3061 ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc
     3121 tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta
     3181 ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg
     3241 aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc acagatgctt
     3301 cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt gcgacagcag
     3361 gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg cggtctagac
     3421 agaaacctgc ctccactagc acctctgggc ccacacccag aaacgctggt gaaagtaaaa
     3481 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3541 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3601 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3661 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3721 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3781 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3841 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3901 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3961 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4021 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4081 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4141 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4201 agacagatcg ctgagatagg tgcctcactg attaagcatt ggggccctga ctacaaagac
     4261 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagggccct
     4321 caccaccacc atcaccacta ataataagga tcccccgacc tcgacctctg gctaataaag
     4381 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     4441 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     4501 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     4561 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     4621 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     4681 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     4741 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     4801 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     4861 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     4921 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     4981 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     5041 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     5101 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     5161 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     5221 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     5281 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     5341 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     5401 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     5461 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     5521 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     5581 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     5641 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     5701 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     5761 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     5821 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     5881 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     5941 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     6001 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     6061 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     6121 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     6181 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6241 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     6301 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     6361 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6421 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6481 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     6541 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     6601 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     6661 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6721 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6781 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6841 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6901 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6961 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7021 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7081 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     7141 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7201 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7261 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7321 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7381 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7441 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7501 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7561 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7621 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7681 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7741 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7801 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8521 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8581 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8641 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8701 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8761 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8821 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8881 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     8941 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     9001 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     9061 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     9121 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
     9181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     9421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     9481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     9541 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     9601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     9661 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     9721 taagcagagc tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg
     9781 agggccagct gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt
     9841 ggatcggaaa cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc
     9901 gcatcgaccg gatcggaaaa cct
//
