LOCUS       sequence-224552-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kockdown of miR-124.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Akerblom M, Sachdeva R, Barde I, Verp S, Gentner B, Trono D, 
            Jakobsson J
  TITLE     MicroRNA-124 is a subventricular zone neuronal fate determinant.
  JOURNAL   J Neurosci. 2012 Jun 27;32(26):8879-89. doi: 
            10.1523/JNEUROSCI.0558-12.2012.
  PUBMED    22745489
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0558-12"; journalName: "J Neurosci"; date: 
            "2012-06-27- 27"; volume: "32"; issue: "26"; pages: "8879-89"
COMMENT     Sequence Label: pLV.CMV.GFP.miR124.sponge
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    161..278
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        301..604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        605..808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     759..779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     805..829
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      898..907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgncaggcc cgaaggatag aagaagaagg tggagagaga gacagagaca gatccattcg
       61 attagtgaac ggatctcgac ggtatcggca acaattaata gactggatgg aggcggataa
      121 agttgcagga ccacttctgc gctcggccct tccggttaac ttttaaaaga aaagggggga
      181 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      241 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgataagctt gggagttccg
      301 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      361 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      421 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      481 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      541 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      601 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      661 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      721 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      781 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      841 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc accggtcgcc
      901 accatggtga gcnaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      961 gna
//
