LOCUS       sequence-224554-        9743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CPN60-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..9743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2389..2400
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             3277..4062
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4069..4134
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4141..4158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4194..4249
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4248..4267)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6485..6503)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6571..6675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6676..6744,6745..7536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7707..8295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8196..8215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8449..8466
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8583..8604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8619..8624,8625..8642,8643..8649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8657..8673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8662..8684
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8681..8697
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8681..8697
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        8968..9347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9348..9551
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9502..9522
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9548..9572
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccatc
      421 aagaagaagc ggagccccaa caacaagaac cggctgttca tcaacaagcg gctgaagtac
      481 atcaacagca agatcatcag ccggcggaaa gaaaactacg tgaagatgaa gatgaccgag
      541 aacaaagtga agggcaagga catcatctac ggcaacgagt gccggaacga gctgctgaag
      601 ggcatcctga ccgtgtccga cgtggtcaag ctgaccctgg gccccagagg cagaaacgtg
      661 ctgctggaaa aagagtacgg cagccctctg atcatcaacg acggcgtgac aatcgccaag
      721 aatatcgccc tgaaggaccg gaagaaaaac aacggcgtga agctgatgca ggaaagcacc
      781 aatatcgcca acgacaaggc cggcgacggc accagctcta ccgccctgat gaccgccacc
      841 atcaccaaga agggaatcga gcaggtcaac cggaaccaca accccatccc catccagcgg
      901 ggcatccagc tggcctccaa gatgatcatt gagaagatca agagcctgag cacccctatc
      961 aagacctata aggacatcct gaacattgcc accattgcca gcaacaacga cgtgcacatg
     1021 ggccagatca ttgccaacgc ctacgacaag ctgggcaaga acgccgccat tatcctggac
     1081 gacaacgccg acatcaacga taagctggaa ttcaccgagg gctacaactt cgaccggggc
     1141 atcatcaacc cctacctgct gtacaacgag aacaaggact acatcgagta cagcaacgtg
     1201 gccaccctga tcaccgacca gaacatcgac aacatccaga gcatcctgcc catcctggaa
     1261 atctttgcca agaacaagca gcccctgtgc atcattgccg acgacttcag caacgaggtg
     1321 ctgcagaccc tgattattaa caagctgaaa ggggccatca aggtggtgcc catcagagcc
     1381 cctagcttcg gcgaccggcg gaaggactac ctgaaggatc tgtgcatcgt gaccaactcc
     1441 aagtacatct ccgccgacgt gggcctggac ctgaacaacc tgcacaacaa catggccagc
     1501 ttcgacaaca actacctgag cctgctgggc agcgccaata ccctgatcgt gaagaaggac
     1561 agaaccagcc tgattaccaa agaagagtac aagaaagaga tcgacgagcg gatcaacgtc
     1621 ctgaagaaag agtatgagga aaccacctct aagtacgaca aagagaagct gaacgagaga
     1681 atcgccgccc tgagcggcgg cattgccaag atcctgatcg gcggaaacag cgagacagag
     1741 cagaaagagc ggaagtttaa gtacgaggac gccaccaacg ccgtgaagtc cgccatcgac
     1801 atcggctacg tgccaggcgg cggagtgacc tatctggaaa tcatcaagag caacttcatc
     1861 caggaaatcc acaagaagat cgaggaagat ctccagatca gctccaacaa cgatgagaag
     1921 aagtacctcg agctgatcgg caacctggaa agcgagatgg aactgcagaa gatgggcgcc
     1981 aacatcgtgg tgtccagcct ggacgtgatc accaagcaga tcgccgacaa cgctggcgtg
     2041 aacggcgaca atgtcgtgaa gatcatcctg aatagcaagg ataagtacgg cttcggctac
     2101 gacgtcaaca ccaacaagtt cgtgaacatg gtcgagaagg ggatcatcga cagcaccaac
     2161 gtgatcatct ccgtgatcaa gaacagctgc tctatcgcca gcatggtgct gaccaccgag
     2221 tgcatgatgg tggaccacga gaagaaggat aagggaatcc tggacagcag catcaactcc
     2281 ccaaactacc tgtccaagca ccggcggacc tacaagcaca agctgcacga cgacgaggac
     2341 accgacgagg atgacgaaga ggacgaggac gatgaggacg acgaagatga tgaggatgac
     2401 gacgactacg atgacgaaga tgaagaggac gaagaggatg aagaggacga ggatgacgag
     2461 gacgatgagg atagcatgaa cgacggctac aattacgacg aaggcgcgcc gtcgacctcc
     2521 atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga
     2581 gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc
     2641 tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc
     2701 aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag
     2761 tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg
     2821 aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg
     2881 ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc
     2941 tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta
     3001 ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg
     3061 aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc acagatgctt
     3121 cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt gcgacagcag
     3181 gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg cggtctagac
     3241 agaaacctgc ctccactagc acctctgggc ccacacccag aaacgctggt gaaagtaaaa
     3301 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3361 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3421 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3481 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3541 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3601 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3661 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3721 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3781 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3841 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3901 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3961 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4021 agacagatcg ctgagatagg tgcctcactg attaagcatt ggggccctga ctacaaagac
     4081 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagggccct
     4141 caccaccacc atcaccacta ataataagga tcccccgacc tcgacctctg gctaataaag
     4201 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     4261 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     4321 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     4381 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     4441 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     4501 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     4561 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     4621 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     4681 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     4741 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     4801 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     4861 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     4921 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     4981 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     5041 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     5101 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     5161 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     5221 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     5281 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     5341 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     5401 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     5461 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     5521 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     5581 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     5641 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     5701 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     5761 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     5821 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     5881 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     5941 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     6001 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6061 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     6121 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     6181 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6241 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6301 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     6361 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     6421 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     6481 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6541 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6901 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     6961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7201 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7561 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7681 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7741 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7801 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7861 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7921 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8041 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8221 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8281 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8341 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8401 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8461 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8521 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8581 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8641 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8701 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     8761 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     8821 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     8881 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     8941 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
     9001 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9061 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     9241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     9301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     9361 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     9421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     9481 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     9541 taagcagagc tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg
     9601 agggccagct gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt
     9661 ggatcggaaa cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc
     9721 gcatcgaccg gatcggaaaa cct
//
